Contribution of oocyte source and culture conditions to phenotypic and transcriptomic variation in commercially produced bovine blastocysts

Plourde, Dany; Vigneault, Christian; Lemay, Alexandra; Breton, Lévéke; Gagné, Dominic; Laflamme, Isabelle; Blondin, Patrick; Robert, Claude

doi:10.1016/j.theriogenology.2012.01.027

Cited by 27 publications

(19 citation statements)

References 52 publications

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“…Culture media produce adverse effects on the structure and function of mitochondria, with consequences for the quality of embryos produced in vitro (Crosier et al 2001, Abe et al 2002a. The expression of genes associated with embryo viability may be modified (Rizos et al 2003, Plourde et al 2012. However, variations in mitochondrial function in association with immature structure have also been observed in embryos produced in vivo (Crosier et al 2001, Fair et al 2001, Abe et al 2002a.…”

Section: Introductionmentioning

confidence: 99%

“…Based on our previous study (Plourde et al 2012), we hypothesized that vitamin K 2 could rescue dysfunctional mitochondria, which would translate into a higher percentage of embryos reaching the blastocyst stage. The objectives of this study were to characterize, for first time, the effects of vitamin K 2 on bovine embryo development and mitochondrial function.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of bovine in vitro embryo production by vitamin K2 supplementation

et al. 2014

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Mitochondria play an important role during early development in mammalian embryos. It has been shown that properly controlled follicular preparation increases the likelihood of in-vitro-produced bovine embryos reaching the blastocyst stage and that competent embryos exhibit heightened expression of genes associated with mitochondrial function. We hypothesized that apparently incompetent embryos could be rescued by restoring mitochondrial function. It has been shown that vitamin K 2 (a membrane-bound electron carrier similar to ubiquinone) can restore mitochondrial dysfunction in eukaryotic cells. The aim of this study was therefore to investigate the effects of vitamin K 2 on bovine embryonic development in vitro. The vitamin was found most effective when added 72 h after fertilization. It produced a significant (P!0.05) increase in the percentage of blastocysts (C8.6%), more expanded blastocysts (C7.8%), and embryos of better morphological quality. It improved the mitochondrial activity significantly and had a measurable impact on gene expression. This is the first demonstration that current standard conditions of in vitro production of bovine embryos may be inadequate due to the lack of support for mitochondrial function and may be improved significantly by supplementing the culture medium with vitamin K 2 .

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

et al. 2014

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“…Moreover, mitochondria are inherited only from the maternal side. Mitochondrial functions have been associated with developmental competence (Dummollard et al, 2009;Van Blerkom, 2011) and culture conditions have been shown to impact mitochondrial functions (Plourde et al, 2012). Since precise quantification of the number of mitochondria per oocyte or per blastomere is technically challenging to do, mtDNA copy number is most often used as an alternate quantification method although copy number is highly variable depending on the cell type, stage of development and species.…”

Section: Reviewmentioning

confidence: 99%

“…The adverse effects of media composition on the structure and function of mitochondria in relation with embryonic quality have been described at the ultrasstructure level (Crosier et al, 2001;Fair et al, 2001;Abe et al, 2002) as well as from gene expression profiling (Rizos et al, 2003;Plourde et al, 2012). Culture conditions, especially the presence of serum which contains growth factors and lipids have been found to impact embryonic developmental kinetics, morphology, developmental potential and capacity to survive cryopreservation (Abe et al, 2002).…”

Section: Reviewmentioning

confidence: 99%

Mitochondrial contributions to oocyte and embryonic quality in bovine

Baldoceda¹,

Robert²

2017

SPERMOVA

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RESUMENLas mitocondrias son orgánelos muy poderosos involucrado en varias funciones vitales en las células, manteniendo principalmente el equilibrio entre la síntesis de ATP y el estrés oxidativo durante el desarrollo embrionario temprano en mamíferos. Se ha demostrado que alteraciones de la función mitocondrial en el modelo bovino podría llegar a afectar la competencia de desarrollo de ovocitos y embriones. En esta revisión, analizamos las implicaciones del papel mitocondrial en el desarrollo temprano exitoso a través de estudios recientes sobre ovocitos y embriones bovinos. Palabras clave: mitocondria, ovocito, embrión, bovino ABSTRACTMitochondria are powerhouse organelles involved in several vital functions in cells, mainly maintaining balance between ATP synthesis and oxidative stress during early development in mammalian embryos. It has been shown that impaired mitochondrial function in bovine model might reach failure in oocyte and embryo developmental competence. In this review, we analyse implications of mitochondrial role on successful early development through recent studies on bovine oocytes and embryos.

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“…However, intrinsic differences were found between OPU oocytes and oocytes from slaughtered ovaries (Merton et al, 2003;Lopes et al, 2006;Presicce et al, 2010;Plourde et al, 2012). On the other hand, the quality of the in vitro-produced embryos from sex-sorted sperm may be affected due to the stress inherent to the sex-sorting process (Rath and Johnson, 2008); nevertheless, the results in this regard are not conclusive yet.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem

Serrano¹

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Esta tesis ha sido propuesta para Mención Internacional en el título de Doctor en virtud de la siguiente estancia de investigación en el extranjero:• Institute of farm animal genetic, Friedrich-Loeffler Institute, Mariensee, Alemania.Supervisor: Dr. Detlef Rath.Periodo de duración: del 4 de mayo al 15 de octubre de 2009. AGRADECIMIENTOSLas tesis doctorales tienen una cosa en común que las hace épicas; buena parte del camino debe recorrerse en solitario. Sin embargo, sin la ayuda y la hospitalidad de las personas que vamos encontrando por el largo y sinuoso viaje iniciático que supone una tesis doctoral, nos hariamos viejos sin llegar lejos. Sirvan estas líneas para mostrar mi agradecimiento a todos los compañeros y amigos que me han ayudado y han hecho que esta tesis fuera posible.En primer lugar, a mis directores de tesis Miguel Ángel Silvestre y Ignacio Salvador, por darme la posibilidad de trabajar y aprender a su lado y de progresar en el mundo de la investigación.A mis compañeros de laboratorio, Elena Megual, Empar Raga, Empar García-Rosselló, Richard Lopera, Javier Ahumada, Manuel Francisco Simao y Ángel Picó, por su inestimable contribución durante la realización de los experimentos.Al Profesor Detlef Rath, por haberme acogido desinteresadamente en su grupo de investigación y haber puesto a mi disposición los medios necesarios para aprender nuevas técnicas y realizar experimentos, y al Profesor Salvador Ruiz y a los compañeros del Institut für Nutztiergenetik que me ayudaron durante mi estancia en Mariensee, especialmente a Klaus-GerdHadeler, Javier Zaraza y Miguel Velázquez.Al Profesor Jesus Yáñiz y al Profesor Fernando López-Gatius, por su valiosa asistencia técnica en los primeros pasos de esta tesis.A la Asociación Frisona Valenciana y a la Granja S.A.T. San Ramón, por el apoyo prestado durante la gestación y el desarrollo de este proyecto.A los compañeros y amigos del CITA, por su contribución directa/o indirecta a este trabajo y por haber compartidos tantos buenos momentos.Por último, a mis amigos y a mi familia, en especial a mis padres, por su apoyo, ánimo y estima incondicional. Cuando emprendas el viaje hacia

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Contribution of oocyte source and culture conditions to phenotypic and transcriptomic variation in commercially produced bovine blastocysts

Cited by 27 publications

References 52 publications

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

Improvement of bovine in vitro embryo production by vitamin K2 supplementation

Mitochondrial contributions to oocyte and embryonic quality in bovine

Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem

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