Contribution of nitrogen fixation to first year <i>Miscanthus</i> × <i>giganteus</i>

Keymer, Daniel P.; Kent, Angela D.

doi:10.1111/gcbb.12095

Cited by 58 publications

(59 citation statements)

References 48 publications

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“…The difference in community structure between rhizosphere communities of the two host species is small, though statistically significant. Our previous study (Keymer & Kent, 2014), which demonstrated a 16% contribution from BNF to the nitrogen uptake during the first year of M. 9 giganteus growth, was conducted at Urbanathe site with the highest average soil N content and lowest average nifH abundance. (n = 207 rhizosphere samples, n = 202 for endophytic compartment samples).…”

Section: Discussionmentioning

confidence: 99%

“…Extraction of endophytic community DNA was carried out as described by Keymer & Kent (2014), a modification of the process described by Brulc et al (2009). Adhering rhizosphere soil was eluted from the root surface by vigorously shaking the Nalgene bottles.…”

Section: Sample Processingmentioning

confidence: 99%

“…The gene was amplified from the extracted and purified microbial DNA using primers PolF (5 0 -FAM-TGCGAYCCSAARGCBGACTC-3 0 ) and PolR (5 0 -HEX-ATSGCCATCATYTCRCCGGA-3 0 ), which generate amplicons of approximately 360 base pairs (sequence position 115-476 relative to A. vinelandii nifH coding sequence (GenBank accession number M20568)) (Poly et al, 2001). PCRs were conducted as described in (Keymer & Kent, 2014). PCRs were conducted as described in (Keymer & Kent, 2014).…”

Section: Diazotroph Community Structurementioning

confidence: 99%

“…The PolF/PolR primers were chosen for effective performance for fragment analysis as well as qPCR for the communities of interest in agricultural soils (Gaby & Buckley, 2012. Purified amplicons were digested for 16 h at 37°C using the restriction enzyme MboII and MnII following the procedures recommended by New England Biolabs (Beverly, MA) (Keymer & Kent, 2014). The resulting amplicons were purified with MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA).…”

Section: Diazotroph Community Structurementioning

confidence: 99%

“…The composition of diazotroph communities in the rhizosphere and the endophytic compartment varies with edaphic and management variables, as well as between hosts. We have also shown that 16% of the nitrogen in the first-year growth of M. 9 giganteus was derived from biological nitrogen fixation (BNF) (Keymer & Kent, 2014). Among diazotroph communities associated with native and uncultivated relatives of M. 9 giganteusnamely M. floridulus and M. sinensisrhizosphere communities have been shown to be affected largely by edaphic factors in the rhizosphere but endophytic communities to vary primarily between host plant species in the endophytic compartment (Li, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Edaphic correlates of feedstock‐associated diazotroph communities

2018

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Miscanthus × giganteus and Panicum virgatum are potential promising bioenergy feedstock crops suitable for the temperate zone. The energy efficiency and sustainability of bioenergy production could be improved by reducing their fertilizer inputs – particularly energy intensive nitrogen fertilizers. Miscanthus is known to benefit from nitrogen fixation by associative diazotrophs. However, because the effects of edaphic‐, management‐, and plant‐related factors on feedstock‐associated diazotroph communities have not yet been characterized, it is not currently possible to optimize the nitrogen contribution to feedstock crops from associated diazotroph communities. To address this critical knowledge gap, we characterized the bacterial and diazotroph communities in the rhizosphere and endophytic compartments of both species at eight research sites across Illinois. We also quantified the nifH gene abundance in the rhizosphere soil as well as a range of soil chemistry parameters at these sites. Multivariate statistical analyses revealed that diazotroph and bacterial communities in the rhizosphere varied primarily among sites, with very small differences between host species. Conversely, diazotroph and bacterial communities in the endophytic compartments differed significantly between plant species, but did not vary substantially among sites. Finally, nifH gene abundance in the rhizospheres of both species varied substantially from site to site and was positively correlated with soil iron concentration as well as soil ammonium concentration, and negatively correlated with abundance of other soil nutrients including calcium, total nitrogen, and nitrates. These results indicate the potential edaphic drivers of associative diazotroph communities in feedstock rhizospheres and suggest that manipulating bioavailable iron content in the soil is a potential direction for investigating the optimization of these communities to improve their nitrogen contribution to crops.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Processingmentioning

confidence: 99%

Section: Diazotroph Community Structurementioning

confidence: 99%

Section: Diazotroph Community Structurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Edaphic correlates of feedstock‐associated diazotroph communities

2018

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The Role of the Phytomicrobiome in Maintaining Biofuel Crop Production in a Changing Climate

Ilangumaran

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Smith

2017

Microbes for Climate Resilient Agriculture

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Diversification and use of bioenergy to maintain future grasslands

Donnison

Fraser

2016

Food and Energy Security

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Grassland agriculture is experiencing a number of threats including declining profitability and loss of area to other land uses including expansion of the built environment as well as from cropland and forestry. The use of grassland as a natural resource either in terms of existing vegetation and land cover or planting of new species for bioenergy and other nonfood applications presents an opportunity, and potential solution, to maintain the broader ecosystem services that perennial grasslands provide as well as to improve the options for grassland farmers and their communities. This paper brings together different grass or grassland‐based studies and considers them as part of a continuum of strategies that, when also combined with improvements in grassland production systems, will improve the overall efficiency of grasslands as an important natural resource and enable a greater area to be managed, replanted or conserved. These diversification options relate to those most likely to be available to farmers and land owners in the marginally economic or uneconomic grasslands of middle to northern Europe and specifically in the UK. Grasslands represent the predominant global land use and so these strategies are likely to be relevant to other areas although the grass species used may vary. The options covered include the use of biomass derived from the management of grasses in the urban and semi urban environment, semi‐natural grassland systems as part of ecosystem management, pasture in addition to livestock production, and the planting and cropping of dedicated energy grasses. The adoption of such approaches would not only increase income from economically marginal grasslands, but would also mitigate greenhouse gas emissions from livestock production and help fund conservation of these valuable grassland ecosystems and landscapes, which is increasingly becoming a challenge.

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Contribution of nitrogen fixation to first year Miscanthus × giganteus

Cited by 58 publications

References 48 publications

Edaphic correlates of feedstock‐associated diazotroph communities

Edaphic correlates of feedstock‐associated diazotroph communities

The Role of the Phytomicrobiome in Maintaining Biofuel Crop Production in a Changing Climate

Diversification and use of bioenergy to maintain future grasslands

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