Contribution of Microbial Acclimation to Lignite Biomethanization

Abstract

Understanding the bioconversion process of lignite to methane is an important contribution to the establishment of effective utilization of the existing coal resources and the acquisition of environmental biofuels. Microorganisms from coal seams were acclimatized using lignite as the sole carbon source to improve the bioavailability of coal. The contribution of acclimation to biomethanation was discussed from the aspects of mathematical models, the changes of key intermediates in the liquid phase, and the dynamic evolution of the community structure. The results showed that the anaerobic digestion (AD) efficiency of lignite was significantly improved after acclimation. The methane yield revealed that the methane-producing capacity of the bacteria and the bioavailability of lignite were enhanced. The data of 16S rDNA also suggested that the diversity of the bacterial and archaeal communities increased significantly. The stability of the AD system was enhanced. The abundance and types of bacterial genera that had a symbiosis with methanogens increased. The gene function abundance and the reading number of the bacteria in terms of membrane transport, cell motility, and xenobiotic biodegradation and metabolism were significantly improved with acclimation. This study provides an experimental basis for microbially enhancing coalbed methane (MECBM) and clean energy production.

Subsurface Microbial Invasion Affects the Microbial Community of Coal Seams

“…Similarly, Pytlak et al found that microbial groups from different sources would produce different intensities of coal seam degradation. Other studies also found that exogenous microorganisms, both acclimated and environmental, influenced microbial activity. In addition, the enhanced bacterial activity caused by interference of soil extraction may not only be caused by microbial invasion.…”

“…We hypothesized that the activities of multiple functional microorganisms in the shallow soil extractions were enhanced and involved in the biodegradation of alkanes under anaerobic conditions, including nitrate reduction, sulfate reduction, and methanogenic conditions, in the coal samples . Similarly, Zhao et al also found that the addition of strain domestication enhanced the functions of bacteria in membrane transport, cell movement, biodegradation, and metabolism and was conducive to the biodegradation process of coal.…”

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

“…The relative abundance of B-OTU2 ranged 5.62-17.09%. As the secondarily dominant OTU, it showed 99% similarity to Terrisporobacter petrolearius, a typical fermentative bacterium (Cabezas et al, 2020) utilizing monosaccharide substrates such as glucose, fructose, and maltose, and producing metabolites such as acetic acid and CO 2 (Zhao et al, 2020). The relative abundance of B-OTU2 was generally maintained above 12% after the onset of anaerobic digestion, with three peaks occurring on the 20th, 60th, and 90th day, respectively.…”

Research Progress and Prospects on Microbial Response and Gas Potential in the Coal Gasification Process

“…Therefore, some studies have domesticated microorganisms to maximize their metabolic potential [ 39 ]. Unable to adapt to the current environment, some microorganisms are iterated or metabolized, while others gradually adapt and eventually form a more targeted microbial community structure [ 40 ].…”