Contribution of magnetic particles in molecular diagnosis of human viruses

Khizar, Sumera; Al‐Dossary, Amal A.; Zine, Nadia; Jaffrézic‐Renault, Nicole; Errachid, Abdelhamid; Elaı̈ssari, Abdelhamid

doi:10.1016/j.talanta.2022.123243

Cited by 19 publications

(7 citation statements)

References 110 publications

(111 reference statements)

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“…Magnetic nanoparticle-based capture is widely used in DNA sensors owing to their good dispersion, low cost, easy separation, purification in buffer systems, and signal detection [ 40 ]. However, signal detection requires additional development processes for fluorescent, electrochemical, or chemiluminescent detection [ 22 , 41 , 42 ]. In the STAT-DNA system, we did not use magnetic nanoparticles for their magnetically controllable properties, such as aggregation, purification, and separation.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Kim

et al. 2022

Biosensors

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Rapid, simple, and inexpensive diagnostic point-of-care tests (POCTs) are essential for controlling infectious diseases in resource-limited settings. In this study, we developed a new detection system based on nanoparticle–DNA aggregation (STat aggregation of tagged DNA, STAT-DNA) to yield a visual change that can be easily detected by the naked eye. This simplified optical detection system was applied to detect hepatitis C virus (HCV). Reverse transcription-polymerase chain reaction (RT-PCR) was performed using primers labeled with biotin and digoxigenin. Streptavidin-coated magnetic particles (1 μm) and anti-digoxigenin antibody-coated polystyrene particles (250–350 nm) were added to form aggregates. The limit of detection (LoD) and analytical specificity were analyzed. The STAT-DNA results were compared with those of the standard real-time PCR assay using serum samples from 54 patients with hepatitis C. We achieved visualization of amplified DNA with the naked eye by adding nanoparticles to the PCR mixture without employing centrifugal force, probe addition, incubation, or dilution. The LoD of STAT-DNA was at least 101 IU/mL. STAT-DNA did not show cross-reactivity with eight viral pathogens. The detection using STAT-DNA was consistent with that using standard real-time PCR.

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Section: Discussionmentioning

confidence: 99%

Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Kim

et al. 2022

Biosensors

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“…[77][78][79][80] The advantage of microenvironmental stimuli-responsive-biomaterials is their precise responsiveness to pathological conditions, but the disadvantage of such materials is that they are difficult to control. [81] The utilization of microenvironmental stimuli-biomaterials necessitates the incorporation of adaptable functional moieties that respond to microenvironmental stimuli, thereby allowing for dynamic alterations in their properties. From the vantage point of biomaterials themselves, the realm of biomaterials encompasses three primary classifications: inorganic biomaterials, organic biomaterials, and biomimetic biomaterials (Figure 3C-E).…”

Section: Biomaterials For Inflammatory Bone Lossmentioning

confidence: 99%

Tailoring Biomaterials Ameliorate Inflammatory Bone Loss

Cheng,

Wang,

Zhou

et al. 2024

Adv Healthcare Materials

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Inflammatory diseases, such as rheumatoid arthritis, periodontitis, chronic obstructive pulmonary disease and celiac disease, disrupt the delicate balance between bone resorption and formation, leading to inflammatory bone loss. Conventional approaches to tackle this issue encompass pharmaceutical interventions and surgical procedures. Nevertheless, pharmaceutical interventions exhibit limited efficacy, while surgical treatments impose trauma and significant financial burden upon patients. Biomaterials show outstanding spatiotemporal controllability, possess a remarkable specific surface area, and demonstrate exceptional reactivity. In the present era, the advancement of emerging biomaterials has bestowed upon us more efficacious solutions for combatting the detrimental consequences of inflammatory bone loss. In this review, we list the advances of biomaterials for ameliorating inflammatory bone loss. Additionally, we summarize the advantages and disadvantages of various biomaterials‐mediated strategies. Finally, we analyze the challenges and perspectives of biomaterials. This review aims to provide new possibilities for developing more advanced biomaterials towards inflammatory bone loss.This article is protected by copyright. All rights reserved

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“…MNPs with proper surface functionalization are especially suitable for the development of lab-in-chip biosensors for easy, reliable, and cost-effective POC diagnostic facilities. In addition, MNPs are widely used in the molecular diagnosis of human viruses [ 128 ] and other infectious pathogens [ 129 ]; MNP-based biosensors are also an extremely promising form in highly accurate POC SARS-CoV-2 detection [ 130 , 131 , 132 ]. In this review, we briefly highlight the following biological objects, which have been recently analyzed using MNP-based POC devices: SARS-CoV-2, Human chorionic gonadotropin (hCG), the typical oral pathogen of Streptococcus, foodborne bacteria in food and environmental samples ( Listeria monocytogenes , Campylobacter jejuni , and Staphylococcus aureus ), pathogenic bacteria ( Escherichia coli ( E. coli ), and Staphylococcus aureus ( S. aureus ), the food pathogen Vibrio parahaemolyticus ), the bacteria Acinetobacter baumannii , and influenza A H1N1 virus ( Table 1 ).…”

Section: Diagnostic Application Of Mnpsmentioning

confidence: 99%

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

et al. 2023

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In the last few decades, point-of-care (POC) sensors have become increasingly important in the detection of various targets for the early diagnostics and treatment of diseases. Diverse nanomaterials are used as building blocks for the development of smart biosensors and magnetite nanoparticles (MNPs) are among them. The intrinsic properties of MNPs, such as their large surface area, chemical stability, ease of functionalization, high saturation magnetization, and more, mean they have great potential for use in biosensors. Moreover, the unique characteristics of MNPs, such as their response to external magnetic fields, allow them to be easily manipulated (concentrated and redispersed) in fluidic media. As they are functionalized with biomolecules, MNPs bear high sensitivity and selectivity towards the detection of target biomolecules, which means they are advantageous in biosensor development and lead to a more sensitive, rapid, and accurate identification and quantification of target analytes. Due to the abovementioned properties of functionalized MNPs and their unique magnetic characteristics, they could be employed in the creation of new POC devices, molecular logic gates, and new biomolecular-based biocomputing interfaces, which would build on new ideas and principles. The current review outlines the synthesis, surface coverage, and functionalization of MNPs, as well as recent advancements in magnetite-based biosensors for POC diagnostics and some perspectives in molecular logic, and it also contains some of our own results regarding the topic, which include synthetic MNPs, their application for sample preparation, and the design of fluorescent-based molecular logic gates.

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Contribution of magnetic particles in molecular diagnosis of human viruses

Cited by 19 publications

References 110 publications

Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus

Tailoring Biomaterials Ameliorate Inflammatory Bone Loss

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

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