Contribution of Lignin Peroxidase, Manganese Peroxidase, and Laccase in Lignite Degradation by Mixed White-Rot Fungi

Abstract

The catalytic depolymerization of biological enzymes during biological liquefaction of coal is very important. In this study, the effects of medium types, lignite addition, and mixed culture on lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) secreted by white-rot fungi are investigated. Fifteen strains of white-rot fungi are used for microbial liquefaction of the Zhaotong lignite in Yunnan. The results show that the activities of LiP, MnP, and Lac are highest in the Czapek-Dox medium (DOX), followed by the Sabouraud Maltose Broth (SMB) medium, and are almost zero in the Potato Dextrose Agar (PDA) medium. In the early stage of fungal growth, the medium contains sufficient nutrients, and lignite addition induces an inhibitory effect on extracellular enzyme secretion, whereas in the late growth stage characterized by nutrient deficiency, it exhibits an enhancing effect. For fungi pair combinations, if only single enzyme activity was considered, the positive synergistic effect of Lac in the mixed culture of s.commune and SL203 would be the highest, with the synergistic coefficient of 233.33. While three enzymes were considered, the integrated effects of ZTW07 and SL203 are the highest, with Lac, MnP, and LiP producing synergistic coefficients of 158.795, 1.217, and 1.006, respectively. It can be seen that the mixed culture can greatly improve the enzyme activity of white rot fungi and is one of the effective methods to improve the liquefaction efficiency.

Elaboration of a Phytoremediation Strategy for Successful and Sustainable Rehabilitation of Disturbed and Degraded Land

“…Informed selection of microbial and macrophyte biocatalysts appears therefore to be crucial for successful implementation of a phytoremediation technology, particularly given the importance of inter-kingdom interaction in the process [151,152]. Furthermore, while degradation of carbonaceous pollutants is clearly one objective [33,58,59,252,253], bacteria [47,105,106,254], in particular PGPR [107], microalgae [255,256] and plants all participate in achieving the desired biotransformation and land restoration outcomes [31,32,34,51]. This inter-kingdom mutualism we posit is made possible by the suite of small molecules emanating from the phyllosphere and plant litter, microbial necromass, root exudate, and in situ substrate weathering, which together constitute the rhizosphere metabolome [257][258][259].…”

A newly isolated lignin degradation bacterium Acinetobacter sp. B213 pretreatment effectively improved hydrolysis of corn straw by enhance cellulose retention and reduce lignin degradation inhibitor

“…Laccase activity was measured as previously described (Erkurt et al, 2007). Manganese peroxidase activity was measured by the manganese sulfate method (Shi et al, 2021). Lignin peroxidase activity was measured by using veratryl alcohol as the substrate (Chen et al, 2012).…”

Lignin phenols in sediment cores and its indications of degradation and organic carbon sources of the Yantan Reservoir, China

“…Oxidative degradation of lignin side-chains caused by white-rot fungi often produces Ad/Al ratios > 0.4 (Shi et al 2020). A speci c lignin degradation pathway due to brown-rot fungi leading to demethylation of methoxylated vanillyl and syringyl constituents could mark an increase in the P/(V + S) ratio (Feng et al 2016).…”