Contribution of Lewis blood groups molecules in biofilm formation of Pseudomonas aeruginosa isolated from atopic dermatitis patients.

Abstract


 Biofilm formation is a mechanism for bacterial community defense against insults including antibiotics .In this report we evaluated the potency of Pseudomonas aeruginosa(P. aeruginosa) isolates from atopic dermatitis patients skin as well as stool to colonize different Lewis types saliva , manifested by biofilm formation .
 The bacteria were cultured on tryptose soy broth .96-well polystyrene plate were used .Coating with heat inactivated Le (a), (b) and (c)saliva was performed. Biofilm intensity was measured using crystal violet stained films compared to non –saliva coated situation.
 The results showed a superior capability of most isolates to form biofilm on Le (a) followed by Le (b) saliva. The highest binding mean was for isolate ( 4).
 Le (a) saliva binding (mean ± SD was 0.66± 0.25 for test compared to 0.21± 0.04 for control non coated wells) , p=0.04,cl=0.041-0.864.
 Other isolates demonstrated variable degree of biofilm formation on this substrate .In contrast to Le (c) saliva, Le (b) saliva demonstrated weak biofilm formation .
 We conclude that, among atopic dermatitis patients skins, P. aeruginosa Lec (A) or Lec (B) lectins might be involved in colonization in such patients.
 Key Words:- Lewis blood groups – Pseudomonas aeruginosa -atopic dermatitis– Biofilm