Contribution of individual compartments of the genital tract to oestrogen and testosterone concentrations in ejaculates of the boar

Claus, R.; Schopper, Doris; Hoang-Vu, C.

doi:10.1530/acta.0.1090281

Cited by 41 publications

(28 citation statements)

References 7 publications

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“…Similar results were obtained by Rostalski (2005) and Hoffmann et al (2010). In contrary, Claus et al (1983Claus et al ( , 1985 found higher concentrations of unconjugated oestrogens in seminal plasma compared to blood plasma. Oestrogens from boar semen increase the myometrial contraction frequency in the sows by stimulation of PGF2α release (Claus et al 1987, Claus 1990).…”

Section: Discussionsupporting

confidence: 88%

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Concentrations of oestrogens in blood plasma and seminal plasma of boars during the postpuberal period

Zduńczyk¹,

Janowski²,

Raś³

et al. 2011

Polish Journal of Veterinary Sciences

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Concentrations of oestrogens in the blood plasma and seminal plasma of mature boars are high. However, little is known about their concentrations after reaching sexual maturity. The aim of this study was to determine the concentration of oestrogens in blood plasma and seminal plasma of boars during the postpuberal period. Free and conjugated oestrone and oestradiol-17β were determined by radioimmunoassay in blood from the testicular vein and artery, and peripheral circulation as well as in seminal plasma collected from 18 Polish Landrace boars. The animals were divided into three groups (n = 6) according to age (8, 12 and 16 months, respectively). Oestrone was predominant free and conjugated oestrogen. The highest values of oestrogens were measured in the testicular vein (p ≤ 0.05). The concentrations of oestrogens in seminal plasma did not differ from those found in the peripheral circulation. An age-dependent increase in levels of all four oestrogens (p ≤ 0.05) was observed. This can be associated with biochemical maturation of the reproductive system during the postpuberal period.

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Section: Discussionsupporting

confidence: 88%

“…Concentrations of oestrogens in the blood plasma of mature boars are high and exceed those found in sows during oestrus (Claus and Hoffmann 1980, Claus et al 1983, 1985. However, little is known about their concentrations in blood plasma and seminal plasma during the early postpuberal period in the boar.…”

Section: +mentioning

confidence: 99%

Concentrations of oestrogens in blood plasma and seminal plasma of boars during the postpuberal period

Zduńczyk¹,

Janowski²,

Raś³

et al. 2011

Polish Journal of Veterinary Sciences

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“…The intra-assay coefficient of variation varied between 3.9 and 6.7% depending on concentration and for the interassay coefficient of variation varied between 7 and 18%. Total estrogens (Claus et al 1985) were determined with an antiserum which had been raised against estrone-17-CMO-BSA. It was used at a dilution in the ratio of 1:15 000.…”

Section: Hormone Analysismentioning

confidence: 99%

“…The sensitivity was 73.5 pmol/l plasma, the intra-assay coefficient of variation varied between 2.9 and 3.7% and the interassay coefficient of variation varied between 10.7 and 16%. In addition to total estrogens, the concentrations of 17b-estradiol were determined in two randomly selected male fetuses from weeks 10, 13, and 15 (Claus et al 1985). The antiserum had been raised against 17b-estradiol-6-CMO-BSA.…”

Section: Hormone Analysismentioning

confidence: 99%

Changes of testicular aromatase expression during fetal development in male pigs (sus scrofa)

Haeussler

Wagner²,

Welter³

et al. 2007

Reproduction

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Male pig fetuses secrete considerable amounts of estrogens, but the location of aromatase activity within the fetal testis is not known. The location of aromatase expression was investigated by immunocytochemistry in fetal testes from week 6 (nZ5), weeks 10, 13, and 15 (each: nZ6) of gestation and additionally in neonates (nZ4). Blood was sampled from the umbilical artery of fetuses and jugular vein of neonates. Histological evaluation of testes involved morphological criteria and counting of Leydig cells, Sertoli cells, and gonocytes. Aromatase activity was localized immunocytochemically and quantified by the percentage of positive stained cells within the same cell type. Aromatase expression was further characterized by quantitative RT-PCR. Concentrations of estrogens, testosterone, FSH, and LH were measured in blood plasma. Total estrogens increased from week 10 to a maximum of 31.03 nmol/l in week 15. Increased testosterone concentrations were only measured at week 6 and were paralleled by slightly elevated estrogens. Thereafter, testosterone dropped and was low throughout. The increase of estrogens was not paralleled by a similar increase of FSH and LH but was related to the increase of the total number of Leydig cells. This increase was also found for mRNA expression. Both Leydig cells and gonocytes were identified as contributors to estrogen formation. Gonocytes were the main source of aromatase at week 10, when gene expression by Leydig cells is low due to the preparation of a wave of Leydig cell mitosis. Reproduction (2007) 133 323-330

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“…Furthermore, boar testes secrete large quantities of estrogens, wherein plasma estradiol-17b (E 2 ) levels increase in a stepwise fashion during pubertal development (Allrich et al 1982, Schwarzenberger et al 1993, Estienne et al 2000. In addition, an androgen-binding protein expressed by Sertoli cells could not be identified (Jegou & Le Gac-Jegou 1978), but testosterone concentrations in the tubular fluid are high (Claus et al 1985). Despite accumulating data on boars, there has been very little progress in understanding the function of INSL3, even though INSL3 was first discovered in the boar testis.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

Minagawa¹,

Sagata²,

Pitia³

et al. 2014

Journal of Endocrinology

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Relaxin-like factor (RLF), now mainly known as insulin-like factor 3 (INSL3), is essential for testis descent during fetal development; however, its function in the adult testis is still being elucidated. As a major step toward understanding the as-yet-unknown function of INSL3 in boars, this study aimed to develop a time-resolved fluoroimmunoassay for boar INSL3, characterize the dynamics of INSL3 expression during development, and demonstrate the expression of the INSL3 hormone-receptor system in the testis. All samples were collected from Duroc boars. The sensitivity of the assay system established was 8.2 pg/well (164 pg/ml), and no cross-reactivity with other hormones, such as porcine relaxin, was observed. Circulating INSL3 was shown to increase progressively during development. INSL3 secreted from the Leydig cells was released not only into the blood circulation but also into the interstitial and seminiferous compartments in sufficient concentrations. A testicular fractionation study revealed that its receptor RXFP2 transcripts were expressed mainly in testicular germ cells. In addition, INSL3 bound to the germ cell membranes in a hormone-specific and saturable manner. These results reveal that INSL3 secreted into the interstitial compartment from the Leydig cells is transported into the seminiferous compartments, where its receptor RXFP2 is expressed mainly in the germ cells to which INSL3 binds, suggesting that INSL3 functions as a paracrine factor on seminiferous germ cells.

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Contribution of individual compartments of the genital tract to oestrogen and testosterone concentrations in ejaculates of the boar

Cited by 41 publications

References 7 publications

Concentrations of oestrogens in blood plasma and seminal plasma of boars during the postpuberal period

Concentrations of oestrogens in blood plasma and seminal plasma of boars during the postpuberal period

Changes of testicular aromatase expression during fetal development in male pigs (sus scrofa)

Dynamics of insulin-like factor 3 and its receptor expression in boar testes

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