Contribution of glutaminases to glutamine metabolism and acid resistance in Lactobacillus reuteri and other vertebrate host adapted lactobacilli

Li, Qing; Tao, QianYing; Teixeira, Januana S.; Su, Marcia Shu-Wei; Gänzle, Michael G.

doi:10.1016/j.fm.2019.103343

Cited by 23 publications

(12 citation statements)

References 44 publications

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“…Enterobacter, Escherichia-Shigella, and Lactobacillus were the dominant bacterial genera in early life (Zhang et al, 2018). These populations prefer to colonize carnivore intestines and subsist on oligosaccharides, fats, amino acids, and their derivatives in cubs' food (Varrone et al, 2013;Kong et al, 2018;Li et al, 2019), and are associated with many important energy metabolism pathways (Guo M. et al, 2018); in particular, Lactobacillus is a probiotic genus in early growth of mammals (Williams et al, 2013). The relative proportion of Streptococcus increased in the cub guts after the age of 1 year when consuming the products of fibrous metabolism (Xue et al, 2015;Wang et al, 2016) in supplementary foods.…”

Section: Succession Of Gut Microbial Diversity and Structure During Bmentioning

confidence: 99%

Succession of Gut Microbial Structure in Twin Giant Pandas During the Dietary Change Stage and Its Role in Polysaccharide Metabolism

Zhan

Wang

Xie³

et al. 2020

Front. Microbiol.

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Section: Succession Of Gut Microbial Diversity and Structure During Bmentioning

confidence: 99%

Succession of Gut Microbial Structure in Twin Giant Pandas During the Dietary Change Stage and Its Role in Polysaccharide Metabolism

Zhan

Wang

Xie³

et al. 2020

Front. Microbiol.

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“…The glutaminase of Lactobacillus does not promote the metabolism of glutamine, but instead enhances acid tolerance. The unique presence of glutaminase in host-adapted Lactobacillus provides an additional link between the adaptation of L. reuteri to specific habitats and its function as a probiotic and starter (Li et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Biological characteristics and whole-genome analysis of the potential probiotic, Lactobacillus reuteri S5

Shi

Dong

Cheng

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: Lactobacillus reuteri is the most common intestinal microorganism, colonizing almost all vertebrate intestines. Strain specificity of lactobacillus makes it necessary to analyse and evaluate the strain information and function of potential probiotic strains such as L. reuteri S5. The whole genome of L. reuteri strain S5 was analysed using whole-genome sequencing and bioinformatics. This work shows that L. reuteri S5 is a probiotic strain with excellent probiotic characteristics and has great potential for developing animal feed additives to promote health.

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“…Glu is involved in protein synthesis and other fundamental cellular processes including; glycolysis, gluconeogenesis, and the citric acid cycle [20]. In particular, Glu plays an important role in acid resistance for a number of microorganisms via the Gad system [21,22]. The Gad system is Glu dependent and is the most effective acid resistance mechanism [23,24].…”

Section: Introductionmentioning

confidence: 99%

“…This system is comprised of two components, glutamate decarboxylase (GadA and GadB), and antiporter GadC. Glu is catalyzed to γ-aminobutyric acid (GABA) by glutamate decarboxylase with consumption of one proton in Escherichia coli, Lactobacillus brevis, Bacillus cereus, and Listeria monocytogenes [21][22][23][24][25]. In this process, GABA is exchanged with extracellular Glu by the Glu/GABA antiporter GadC [26,27].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Gad system on Actinobacillus succinogenes during acid stress

Chen

Zhang

Qian

et al. 2021

Syst Microbiol and Biomanuf

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The glutamate decarboxylase (Gad) system is an important amino acid-dependent acid resistance system commonly found in microorganisms. Actinobacillus succinogenes is one of the best natural producers of succinic acid (SA) but lacks glutamate decarboxylase. This study assessed the effects of Gad system introduction into A. succinogenes. The recombinant strains gadB-SW and gadBC-SW were constructed by heterologous expression of gadB alone, or gadB together with gadC, respectively. After 1.0 and 1.5 h of acid stress at pH 4.6, cell survival of gadBC-SW was greater than gadB-SW. The growth of gadB-SW and gadBC-SW was both affected by the expression of heterologous proteins and by γ-aminobutyric acid, with gadBC-SW growth reduced at a neutral pH. SA production in acidic conditions was evaluated by a shake flask and by 3-L bioreactor fermentation. The results showed gadBC-SW to increase SA production by 8.4% in shake flask compared to the parent strain, SW. For a 3-L bioreactor batch fermentation under acidic environment, the highest conversion rate of sugar to SA was observed for gadBC-SW, reaching 96%. However, SA concentration by gadBC-SW was only 47 g/L and 31 g/L at pH 6.5 and pH 6.0, respectively. In summary, the introduction of heterologous gadB and gadC into A. succinogenes not only improved acid tolerance but also influenced the synthesis of SA and added a metabolic burden.

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Contribution of glutaminases to glutamine metabolism and acid resistance in Lactobacillus reuteri and other vertebrate host adapted lactobacilli

Cited by 23 publications

References 44 publications

Succession of Gut Microbial Structure in Twin Giant Pandas During the Dietary Change Stage and Its Role in Polysaccharide Metabolism

Succession of Gut Microbial Structure in Twin Giant Pandas During the Dietary Change Stage and Its Role in Polysaccharide Metabolism

Biological characteristics and whole-genome analysis of the potential probiotic, Lactobacillus reuteri S5

Effect of the Gad system on Actinobacillus succinogenes during acid stress

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