Contribution of fibronectin-binding protein to pathogenesis of<i>Streptococcus equi</i>ssp<i>. zooepidemicus</i>

Li, Yang; Wang, Yang; Ma, Zhe; Zhang, Hui; Li, Yue; Zheng, Jun-xi; Yang, Yue; Chen, Lu; Fan, Hongjie

doi:10.1111/2049-632x.12029

Cited by 14 publications

(7 citation statements)

References 36 publications

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“…Interestingly, it has been reported that in Staphylococcus epidermidis , EmbP is expressed only in the presence of serum . Bacteria possess different mechanisms to recognize fibronectin and other proteins that are present in host‐cell membranes . However, it is still unclear if these molecules are signals that trigger biofilm formation or if they only play a physical role in cell‐surface interactions.…”

Section: Heterologous (Signaling) Molecules That Alter Biofilm Formatmentioning

confidence: 99%

From environmental signals to regulators: Modulation of biofilm development in Gram‐positive bacteria

Mhatre

Gallegos‐Monterrosa

Kovács

2014

J. Basic Microbiol.

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Bacterial lifestyle is influenced by environmental signals, and many differentiation processes in bacteria are governed by the threshold concentrations of molecules present in their niche. Biofilm is one such example where bacteria in their sessile state adapt to a lifestyle that causes several adaptive alterations in the population. Here, a brief overview is given on a variety of environmental signals that bias biofilm development in Gram-positive bacteria, including nutrient conditions, self- and heterologously produced substances, like quorum sensing and host produced molecules. The Gram-positive model organism, Bacillus subtilis is a superb example to illustrate how distinct signals activate sensor proteins that integrate the environmental signals towards global regulators related to biofilm formation. The role of reduced oxygen level, polyketides, antimicrobials, plant secreted carbohydrates, plant cell derived polymers, glycerol, and osmotic conditions are discussed during the transcriptional activation of biofilm related genes in B. subtilis.

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Section: Heterologous (Signaling) Molecules That Alter Biofilm Formatmentioning

confidence: 99%

From environmental signals to regulators: Modulation of biofilm development in Gram‐positive bacteria

Mhatre

Gallegos‐Monterrosa

Kovács

2014

J. Basic Microbiol.

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“…zooepidemicus has also been found to occur in a wide range of other animals and in humans . In China, it is the main pathogen causing disease in swine . Putative virulence factors of S. equi ssp.…”

Section: Introductionmentioning

confidence: 99%

Structural and functional analysis of the fibronectin‐binding protein FNE from Streptococcus equi spp. equi

et al. 2014

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Streptococcus equi is a horse pathogen belonging to Lancefield group C. Infection by S. equi ssp. equi causes strangles, a serious and highly contagious disease of the upper respiratory tract. F I . FNE lacks the canonical Fn-binding peptide repeats observed in many microbial surface components recognizing adhesive matrix molecules. We found that the interaction between FNE and the human GBD is mediated by the binding of the disordered Cterminal region (residues 208-262) of FNE to the 789 F I GBD subfragment. The crystal structure of FNE showed that it is similar to the minor pilus protein Spy0125 of Streptococcus pyogenes, found at the end of pilus polymers and responsible for adhesion. FNE and Spy0125 both have a superimposable internal thioester bond between highly conserved Cys and Gln residues. Small-angle X-ray scattering of the FNE-789 F I complex provided a model that aligns the C-terminal peptide of FNE with the E-strands of the F I domains, adopting the b-zipper extension model observed in previous structures of microbial surface components recognizing adhesive matrix molecule adhesion peptides bound to F I domains. DatabaseThe coordinates of the FNE-aREP3 complex have been deposited at the Protein Data Bank under the code 4PFG. Structured digital abstract• GBD and FNE bind by isothermal titration calorimetry (View interaction)• GBD 789FI and FNE bind by isothermal titration calorimetry (View interaction)• FNE-CT and GBD 789FI bind by isothermal titration calorimetry (View interaction)• GBD and FNE-CT bind by isothermal titration calorimetry (View interaction)• FNE and GBD 789FI bind by molecular sieving (View interaction)• FNE-CT binds to GBD 789FI by pull down (View interaction)• FNE and GBD 789FI bind by x ray scattering (View interaction)• GBD and FNE physically interact by fluorescence polarization spectroscopy (View interaction)• collagen and GBD bind by fluorescence polarization spectroscopy (View interaction)

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“…Two weeks after the second booster dose, the mice in groups 1–3 were inoculated intraperitoneally with 0.2 mL of bacterial suspensions (1.25 × 10 6 cfu/mL, approximately 5 × LD 50 ) obtained from an 18-h culture in THB to evaluate the protection against an S. equi spp. zooepidemicus challenge [ 14 , 15 ]. Another eight mice in Group 4 were injected with 0.2 mL of sterile PBS.…”

Section: Methodsmentioning

confidence: 99%

“…A S. equi spp. zooepidemicus colony was inoculated into 5 mL of THB and was cultivated with shaking at 37 °C for 12 h (late exponential/stationary growth phase) [ 15 ]. The culture was diluted 1:100 with fresh THB broth supplemented with 1% fibrinogen and with either 1% pooled mouse anti-rGroEL serum or 1% pooled non-immunized mouse sera from day 35.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation

Wang

et al. 2016

Vet Res

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Streptococcus equi ssp. zooepidemicus (S. equi spp. zooepidemicus) is an opportunistic pathogen that causes major economic losses in the swine industry in China and is also a threat for human health. Biofilm formation by this bacterium has been previously reported. In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus. Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria. The immunogenicity and protective efficacy of the S. equi spp. zooepidemicus immunoreactive GroEL chaperone protein was further investigated in mice. The protein was expressed in vivo and elicited high antibody titers following S. equi spp. zooepidemicus infections of mice. An animal challenge experiment with S. equi spp. zooepidemicus showed that 75% of mice immunized with the GroEL protein were protected. Using in vitro biofilm inhibition assays, evidence was obtained that the chaperonin GroEL may represent a promising target for the prevention and treatment of persistent S. equi spp. zooepidemicus biofilm infections. In summary, our results suggest that the recombinant GroEL protein, which is involved in biofilm formation, may efficiently stimulate an immune response, which protects against S. equi spp. zooepidemicus infections. It may therefore be a candidate of interest to be included in vaccines against S. equi spp. zooepidemicus infections.

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Contribution of fibronectin-binding protein to pathogenesis ofStreptococcus equissp. zooepidemicus

Cited by 14 publications

References 36 publications

From environmental signals to regulators: Modulation of biofilm development in Gram‐positive bacteria

From environmental signals to regulators: Modulation of biofilm development in Gram‐positive bacteria

Structural and functional analysis of the fibronectin‐binding protein FNE from Streptococcus equi spp. equi

Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation

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