Contribution of Fatty Acids Released From Lipolysis of Plasma Triglycerides to Total Plasma Fatty Acid Flux and Tissue-Specific Fatty Acid Uptake

Teusink, Bas; Voshol, Peter J.; Dahlmans, V.E.H.; Rensen, Patrick C.N.; Pijl, Hanno; Romijn, Johannes A.; Havekes, Louis M.

doi:10.2337/diabetes.52.3.614

Cited by 132 publications

(122 citation statements)

References 33 publications

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“…Nonetheless, the infusion of either triglyceride or free fatty acid bound to albumin does not appear to affect the kinetics of tracer movement from the plasma compartment into heart (4, 47, 52). The rapid interchange of fatty acids hydrolyzed from triglycerides with the free fatty acid pool negates any difference between these two different routes of delivery (47). Furthermore, infusion of [1-3 H]hexadecanol has been used to quantify the kinetics of heart ether-lipid biosynthesis (15) and indicate that the use of high-specific-activity radiotracers was successful in estimating heart lipid biosynthesis or fatty acid uptake (4,35,36,47,52).…”

Section: Animalsmentioning

confidence: 99%

Arachidonic acid incorporation and turnover is decreased in sympathetically denervated rat heart

Patrick¹,

McHowat²,

Rosenberger³

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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. Arachidonic acid incorporation and turnover is decreased in sympathetically denervated rat heart. Am J Physiol Heart Circ Physiol 288: H2611-H2619, 2005 C]20:4 n-6), and kinetic parameters were assessed using a steady-state radiotracer model. Heart norepinephrine and epinephrine levels were decreased 82 and 85%, respectively, in denervated rats, and this correlated with a 34% reduction in weight gain in treated rats. Fatty acid tracer uptake was not significantly different between groups for either tracer, although the dilution coefficient was increased in [1-14 C]20:4 n-6-infused rats, which indicates that less 20:4 n-6 was recycled in denervated rats. In [1-14 C]16:0-infused rats, incorporation rate and turnover values of 16:0 in stable lipid compartments were unchanged, which is indicative of preservation of ␤-oxidation. In [1-14 C]20:4 n-6-infused rats, there were dramatic reductions in incorporation rate (60 -84%) and turnover value (56 -85%) in denervated rats that were dependent upon the lipid compartment. In addition, phospholipase A2 activity was reduced 40% in treated rats, which is consistent with the reduction observed in 20:4 n-6 turnover. These results demonstrate marked reductions in 20:4 n-6 incorporation rate and turnover in sympathetic denervated rats and thereby suggest an effect on lipid-mediated signal transduction mediated by a reduction in phospholipase A2 activity. palmitic acid; signal transduction; phospholipase A 2; catecholamines; phospholipids UNDER NORMAL PHYSIOLOGICAL conditions, the mammalian heart uses palmitic acid (16:0) as a primary source of metabolic energy via ␤-oxidation (11

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Section: Animalsmentioning

confidence: 99%

Arachidonic acid incorporation and turnover is decreased in sympathetically denervated rat heart

Patrick¹,

McHowat²,

Rosenberger³

et al. 2005

American Journal of Physiology-Heart and Circulatory Physiology

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“…Infusion of [ 14 C]palmitate-labelled CM into rats demonstrated substantial CM-TAG oxidation which was decreased only slightly (16%) by co-administration of glucose, implying a preference for CM-TAG oxidation [6]. High rates of label retention of CM prepared from chyle lymph were found in heart (more than skeletal muscle though less than white adipose tissue), and about 3 fold higher than NEFA; the ratio of TAG retention to NEFA retention was higher in heart than other tissues, suggesting a strong cardiac preference for TAG [7]. Gousios [8] perfused rabbit hearts with d<1.006 lipoproteins containing labelled TAG (a density corresponding to both CM and VLDL) and found fractional extraction rates of 15% in the fed state and 30% in hearts from fasted animals.…”

Section: Accepted Manuscriptmentioning

confidence: 92%

“…Infusion of labelled CM-TAG into mice found that 80-90% of the TAG-FA was recovered from plasma, and TAG lipolysis contributed 10-20% of total plasma FA turnover [7]; given the substantial cardiac TAG-FA assimilation in these experiments, the authors proposed a model of very high local FA released from CM-TAG lipolysis [7]. T CM-TAG is seen in physiologic and pathologic situations of metabolic perturbation and redistribution of plasma substrates.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The attachment of mature, active LPL to EC-H"PG GPIHBP [63] permitting plasma TAG hydrolysis [64]. As well as tissue uptake of the liberated FA, there is FA [7]. In heart, a substantial proportion of LPL-released FA are assimilated by cardiomyocytes, through pathways that may or may not be shared with NEFA uptake mechanisms (e.g.…”

Section: Lipoprotein Lipase Mediated Myocardial Tag Uptakementioning

confidence: 99%

“…suggesting that VLDL-TAG may be a minor contributor to energy production, but that luminal lipolysis may also release significant NEFA to the systemic circulation (see [7]). In this study the myocardium was only a minor contributor to total systemic TAG (VLDL) uptake (~3%) and systemic NEFA production (~0.5%) [2].…”

Section: Cardiac Vldl-tag Utilisationmentioning

confidence: 99%

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The role of triacylglycerol in cardiac energy provision

Evans

Hui‐Kang

2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Triacylglycerols (TAG) constitute the main energy storage resource in mammals, by virtue of their high energy density. This in turn is a function of their highly reduced state and hydrophobicity.Limited water solubility, however, imposes specific requirements for delivery and uptake mechanisms on TAG-utilising tissues, including the heart, as well as intracellular disposition. TAGs constitute potentially the major energy supply for working myocardium, both through bloodborne provision and as intracellular TAG within lipid droplets, but also provide the heart with fatty acids (FA) which the myocardium cannot itself synthesise but are required for glycerolipid derivatives with (non-energetic) functions, including membrane phospholipids and lipid signalling molecules. Furthermore they serve to buffer potentially toxic amphipathic fatty acid derivatives.Intracellular handling and disposition of TAGs and their FA and glycerolipid derivatives similarly requires dedicated mechanisms in view of their hydrophobic character. Dysregulation of utilisation can result in inadequate energy provision, accumulation of TAG and/or esterified species, and these may be responsible for significant cardiac dysfunction in a variety of disease states. This review will focus on the role of TAG in myocardial energy provision, by providing FAs from exogenous and endogenous TAG sources for mitochondrial oxidation and ATP production, and how this can change in disease and impact on cardiac function. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 3Key words:heart; triacylglycerol; very-low-density lipoprotein; VLDL; chylomicron; lipid droplet Highlights: Triacylglycerols are supplied to the myocardium within chylomicrons and VLDL  Heart assimilates triacylglycerol-rich lipoproteins by LPL and receptor-mediated routes  Exogenous triacylglycerol-derived fatty acids enter an intracellular lipid pool  Intracardiac triacylglycerol is an important source of fatty acids for oxidation  Dysregulation of triacylglycerol metabolism is associated with cardiac dysfunction

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Metabolic Fuels and Obesity: Carbohydrate and Lipid Metabolism in Skeletal Muscle and Adipose Tissue

Frayn¹,

Blaak²

2005

Clinical Obesity in Adults and Children

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Contribution of Fatty Acids Released From Lipolysis of Plasma Triglycerides to Total Plasma Fatty Acid Flux and Tissue-Specific Fatty Acid Uptake

Abstract: There is controversy over the extent to which fatty acids (FAs) derived from plasma free FAs (FFAs)

Cited by 132 publications

References 33 publications

Arachidonic acid incorporation and turnover is decreased in sympathetically denervated rat heart

Arachidonic acid incorporation and turnover is decreased in sympathetically denervated rat heart

The role of triacylglycerol in cardiac energy provision

Metabolic Fuels and Obesity: Carbohydrate and Lipid Metabolism in Skeletal Muscle and Adipose Tissue

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