Abstract:The final stages of sperm differentiation occur outside the gonad and are not under the genomic control of germ cells. Only sequential interactions with the medium surrounding the sperm are believed to induce the final steps of spermatogenesis. The epididymis, a long tubule with very active secretory and reabsorption functions, is able to create sequential changes in the composition of luminal fluid throughout its length. The chronologies of the changes, which occur on/in the sperm with those in their surround… Show more
“…In mammals, multiple regulators of proteolysis, particularly metallo-and serine proteases and serpins, are present in seminal fluid (17)(18)(19) and have been suggested to function in capacitation and fertilization. For example, the prostate-specific antigen (PSA) is a serine protease that is involved in sperm motility, probably by processing of semenogelins (50).…”
Section: Discussionmentioning
confidence: 99%
“…These classes include protease inhibitors, proteases, lipid modifying enzymes, lectins, CRISPs (cysteine-rich secreted proteins), antimicrobial peptides, and antioxidants (reviewed in refs. [17][18][19]. These protein classes have proposed involvements in semen coagulation, sperm motility, sperm maturation, sperm acrosome reaction, sperm viability, sperm capacitation, and control of immune activity within the female reproductive tract of mammals (reviewed in refs.…”
mentioning
confidence: 99%
“…These protein classes have proposed involvements in semen coagulation, sperm motility, sperm maturation, sperm acrosome reaction, sperm viability, sperm capacitation, and control of immune activity within the female reproductive tract of mammals (reviewed in refs. [17][18][19].…”
The constituents of seminal fluid are a complex mixture of proteins and other molecules, most of whose functions have yet to be determined and many of which are rapidly evolving. As a step in elucidating the roles of these proteins and exposing potential functional similarities hidden by their rapid evolution, we performed comparative structural modeling on 28 of 52 predicted seminal proteins produced in the Drosophila melanogaster male accessory gland. Each model was characterized by defining residues likely to be important for structure and function. Comparisons of known protein structures with predicted accessory gland proteins (Acps) revealed similarities undetectable by primary sequence alignments. The structures predict that Acps fall into several categories: regulators of proteolysis, lipid modifiers, immunity͞ protection, sperm-binding proteins, and peptide hormones. The comparative structural modeling approach indicates that major functional classes of mammalian and Drosophila seminal fluid proteins are conserved, despite differences in reproductive strategies. This is particularly striking in the face of the rapid protein sequence evolution that characterizes many reproductive proteins, including Drosophila and mammalian seminal proteins.
“…In mammals, multiple regulators of proteolysis, particularly metallo-and serine proteases and serpins, are present in seminal fluid (17)(18)(19) and have been suggested to function in capacitation and fertilization. For example, the prostate-specific antigen (PSA) is a serine protease that is involved in sperm motility, probably by processing of semenogelins (50).…”
Section: Discussionmentioning
confidence: 99%
“…These classes include protease inhibitors, proteases, lipid modifying enzymes, lectins, CRISPs (cysteine-rich secreted proteins), antimicrobial peptides, and antioxidants (reviewed in refs. [17][18][19]. These protein classes have proposed involvements in semen coagulation, sperm motility, sperm maturation, sperm acrosome reaction, sperm viability, sperm capacitation, and control of immune activity within the female reproductive tract of mammals (reviewed in refs.…”
mentioning
confidence: 99%
“…These protein classes have proposed involvements in semen coagulation, sperm motility, sperm maturation, sperm acrosome reaction, sperm viability, sperm capacitation, and control of immune activity within the female reproductive tract of mammals (reviewed in refs. [17][18][19].…”
The constituents of seminal fluid are a complex mixture of proteins and other molecules, most of whose functions have yet to be determined and many of which are rapidly evolving. As a step in elucidating the roles of these proteins and exposing potential functional similarities hidden by their rapid evolution, we performed comparative structural modeling on 28 of 52 predicted seminal proteins produced in the Drosophila melanogaster male accessory gland. Each model was characterized by defining residues likely to be important for structure and function. Comparisons of known protein structures with predicted accessory gland proteins (Acps) revealed similarities undetectable by primary sequence alignments. The structures predict that Acps fall into several categories: regulators of proteolysis, lipid modifiers, immunity͞ protection, sperm-binding proteins, and peptide hormones. The comparative structural modeling approach indicates that major functional classes of mammalian and Drosophila seminal fluid proteins are conserved, despite differences in reproductive strategies. This is particularly striking in the face of the rapid protein sequence evolution that characterizes many reproductive proteins, including Drosophila and mammalian seminal proteins.
“…9,10 The dramatic recent progress made in the management of male infertility is largely attributable to the improved microsurgical techniques for the repair of reproductive tract obstruction. 11 However, natural pregnancy occurs in only 15%-50% of these cases after repair of the reproductive tract obstruction.…”
Vasectomy is a simple and reliable method of male contraception. A growing number of men after vasectomy request vasectomy reversal due to various reasons. The pregnancy rate is lower than the patency rate after vasovasostomy and the pregnancy rate is time dependent. In this study, we evaluated the influence of reproductive tract obstruction on expression of epididymal proteins and their restoration after patency. Adult male Wistar rats were studied 30, 60 and 120 days after vasectomy, 30 days after vasovasostomy or after sham operations. Two-dimensional gel electrophoresis, mass-spectrometric technique, multidatabase search, Western blotting and real-time PCR were used to analyze the expression regulation of epididymal proteins. Total integrated intensity and total spot area of autoradiograms showed a consistent downward trend with time after obstruction, and this trend remained after patency. The intensity of the autoradiographic spots in three patency groups showed three trends: a downward trend, similar intensity and an upward trend compared with the correspondent obstruction group, respectively. Further verified experiments on human epididymis 2 (HE2), fertilization antigen-1 (FA-1), clusterin and PH20 demonstrated that compared with the correspondent obstruction group, the translation levels of HE2 and the mRNA transcription levels of HE2 showed an upward trend in patency groups, especially in the groups of obstruction for 60 days where the expression levels of HE2 were significantly upregulated after patency (P,0.05). Reproductive tract obstruction provokes a disregulation of gene expression in the epididymis and this disregulation remained after patency. Successful reversal may recover some proteins and the recovery is time dependent. Obstruction differentially alters mRNA transcription of different proteins and the content of proteins seemed to be easier to be influenced than the gene transcription. Vasovasostomy by modern microsurgical techniques remains the standard technique for vasectomy reversal with which all other methods of vasectomy reversal are compared. If success is defined that sperm can be detected in ejaculate following reversal, patency is over 80% in most microsurgical series, with patency approaching 100% in some reports. 1 However, pregnancies are obtained without assisted reproduction in 30%-75% of couples. 2 In other words, about 25% of couples are affected. Male factors account for about 50% of couples with infertility. 3 Approximately 10%-15% of infertile men suffer from azoospermia-complete absence of sperms in the ejaculate. Among these azoospermic patients, approximately 40% have complete obstruction in the ductal system and hence suffer from obstructive azoospermia. 4 Vasoepididymostomy is performed for congenital, infectious, postvasectomy or idiopathic epididymal obstruction. Following this type of microsurgery, 20%-40% of couples achieve pregnancy through intercourse without the assistance of reproductive techniques. 2 Similar to vasectomy reversal, the pregnancy rate is mu...
“…Another important feature is that during epididymal passage, the spermatozoa are exposed to a varying environment, characterized by high concentration of osmolytes (26,32). Finally, the mature spermatozoa accumulate in the cauda epididymidis where they are stored stable and immotile under hyperosmotic conditions until ejaculation (33).…”
Section: The Sperm's Life From Testis To Eggmentioning
Cytometric methodologies are becoming increasingly important in veterinary andrology as means of assessing sperm function. However, as yet, flow cytometric techniques in veterinary andrology have not kept up in sophistication with those in other areas of biology and medicine. In this brief review, we consider the present state of cytometry in andrological procedures for evaluating the fertility of domestic animal sires. We outline the aspects of sperm physiology, paying particular attention to the changes that take place during the process known as capacitation, which prepares the sperm for interaction with the egg. We then examine briefly but critically the cytometric techniques that are currently in commercial use or are being established in research laboratories for testing sperm characteristics. Current limitations and potential developments in semen assessment are discussed. Recent research knowledge offers possibilities for applying more subtle flow cytometric approaches to distinguish different levels of fertilizing potential in semen samples. For example, linking field fertility data to multiparametric kinetic studies of sperm capacitational changes rather than ''single parameter-single time point'' estimations may reveal that slower rather than rapid changes indicate high fertility. Moreover, the development of multicolor flow cytometric procedures as a means of evaluating multiple functional parameters in individual cells would reduce the uncertainties always inherent in predicting fertility from in vitro sperm evaluation tests. ' 2011 International Society for Advancement of Cytometry Key terms fertility; sperm evaluation; heterogeneity; subpopulations; multicolour cytometry THE main focus of an andrological assessment in veterinary medicine is to ascertain the fertility of a potential sire. Although the most obvious and indeed frequently used general veterinary test is to quantify the number of offspring born to the sire, this is a lengthy and very expensive process. For ''accuracy,'' many inseminations must be carried out, and results must await gestation and birth. Obviously, therefore, development of rapid tests that can be carried out in the laboratory has for long been important. However, as research into the reproductive process has advanced, it has become clear that the life of the fertilizing sperm between its release from the testis and its fusion with the egg is far from simple. Moreover, the population of sperm ejaculated into the female has been revealed as being heterogeneous in functional ability, even if morphologically normal [c.f. reviews (1,2)]. As a result of these findings, tests are being developed to examine functional ability within a heterogeneously responding population (3-5).Given that a normal male ejaculate contains many millions of individual sperm cells, it is natural that these tests are based on cytometry. In this short review, we will outline and comment upon a range of modern and still-developing cytometric methodologies in use or potentially useful for sperm...
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