Contribution à l'étude de l'origine des levures du fromage de Camembert

Baroiller, C.; Schmidt, John L.; Lapadu-Hargues, M.

doi:10.1051/lait:199017

“…Genomic DNA was extracted from ¢ve D. hansenii [12] and D. hansenii strain TL191, isolated from Camembert cheese [18]. DNA from each of these ¢ve strains was used as a template in RAPD, with the F and M series of decamers from Operon Technology (USA).…”

Section: Construction and Characterization Of The Dna Probesmentioning

confidence: 99%

“…Genomic DNA was extracted from ¢ve D. hansenii hansenii strains isolated from Spanish cheese, CLIB 238, CLIB 239 and CLIB 240, previously distinguished from one another by RAPD and mitochondrial DNA (mtDNA) RFLP analysis [12] and D. hansenii strain TL191, isolated from Camembert cheese [18]. DNA from each of these ¢ve strains was used as a template in RAPD, with the F and M series of decamers from Operon Technology (USA).…”

Section: Construction and Characterization Of The Dna Probesmentioning

confidence: 99%

See 1 more Smart Citation

DNA probes specific for the yeast species Debaryomyces hansenii: useful tools for rapid identification

Corredor¹

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

We developed a rapid and sensitive identification method for the halotolerant yeast Debaryomyces hansenii, based on the hybridization of species-specific sequences. These sequences were first identified in a survey of D. hansenii strains by random amplification of polymorphic DNA (RAPD) as giving conserved bands in all isolates tested. Two such conserved RAPD products, termed F01pro and M18pro, were cloned from the type strain CBS 767. The specificity of these probes was assessed by hybridizing them to DNA from various species of yeasts commonly found in cheese. F01pro and M18pro hybridized to the DNA of all D. hansenii var. hansenii tested, but not to DNA of other yeast species including the closely related strain of D. hansenii var. fabryii CBS 789. Hybridization patterns of F01pro and M18pro on digested genomic DNA of D. hansenii indicated that the sequences were repeated in the genome of all D. hansenii var. hansenii tested, and gave distinct polymorphic patterns. The single F01pro probe generated 11 different profiles for 24 strains by restriction fragment length polymorphism, using one restriction enzyme. F01pro represents a new type of repeated element found in fungi, useful for both identification and typing of D. hansenii and, together with M18pro, simplifies the study of this species in complex flora. ß

show abstract

“…Genomic DNA was extracted from five D. hansenii strains of diverse origins: the D. hansenii var. hansenii type strain CBS 767, 3 D. hansenii strains isolated from Spanish cheese, CLIB 238, CLIB 239 and CLIB 240, previously distinguished from one another by RAPD and mitochondrial DNA (mtDNA) RFLP analysis [12] and D. hansenii strain TL191, isolated from Camembert cheese [18]. DNA from each of these five strains was used as a template in RAPD, with the F and M series of decamers from Operon Technology (USA).…”

Section: Resultsmentioning

confidence: 99%

DNA probes specific for the yeast speciesDebaryomyces hansenii: useful tools for rapid identification

Corredor¹,

Davila²,

Gaillardin³

et al. 2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

We developed a rapid and sensitive identification method for the halotolerant yeast Debaryomyces hansenii, based on the hybridization of species-specific sequences. These sequences were first identified in a survey of D. hansenii strains by random amplification of polymorphic DNA (RAPD) as giving conserved bands in all isolates tested. Two such conserved RAPD products, termed F01pro and M18pro, were cloned from the type strain CBS 767. The specificity of these probes was assessed by hybridizing them to DNA from various species of yeasts commonly found in cheese. F01pro and M18pro hybridized to the DNA of all D. hansenii var. hansenii tested, but not to DNA of other yeast species including the closely related strain of D. hansenii var. fabryii CBS 789. Hybridization patterns of F01pro and M18pro on digested genomic DNA of D. hansenii indicated that the sequences were repeated in the genome of all D. hansenii var. hansenii tested, and gave distinct polymorphic patterns. The single F01pro probe generated 11 different profiles for 24 strains by restriction fragment length polymorphism, using one restriction enzyme. F01pro represents a new type of repeated element found in fungi, useful for both identification and typing of D. hansenii and, together with M18pro, simplifies the study of this species in complex flora.

show abstract

“…Previous studies on yeast populations present in cheese were based on the identification of the species with conventional diagnostic testing [2,3,20,25,26]. The use of morphological, physiological and biochemical criteria is timeconsuming.…”

Section: Introductionmentioning

confidence: 99%

Use of specific DNA probes for the rapid characterization of yeasts isolated from complex biotopes

Davila¹,

Diez²,

Corredor³

et al. 2001

View full text Add to dashboard Cite

We describe a rapid method developed to identify yeast species commonly found during cheese ripening. It is based on the isolation of species-specific sequences and their hybridization with coarse preparations of genomic DNA. Several strategies were followed for the construction of probes: PCR amplification of sequences available in databases, random cloning of genomic DNA fragments, specific RAPD fragments and PCR-amplified ribosomal DNA fragments. After validation, the probes were applied to the characterization of 400 yeast strains isolated from various French cheeses. Since the strains had been previously identified with classical diagnostic tests, we were able to compare molecular and conventional identification. In addition, the specific probes for one of the species, Debaryomyces hansenii, were used successfully in colony hybridization experiments. The probes developed here proved to be very useful for the screening of large yeast collections and for the assessment of biodiversity within the yeast flora in cheese. yeast / cheese / molecular identification / hybridization / biodiversityResume -Utilisation de sondes ADN specifiques pour la caracterisation rapide de levures isoIees d'ecosystemes complexes. Au cours de ce travail, nous avons developpe une methode a la fois rapide et sensible pour l'identification des levures couramment isolees pendant l'affinage du fromage. Son principe repose sur l'hybridation de sequences specifiques sur les preparations brutes d' ADN genomique des souches a identifier. Pour la construction des sondes, nous avons du suivre plusieurs strategies differentes: amplification de sequences disponibles dans les bases de donnees, clonage au hasard de sequences d' ADN genomique, isolement de sequences specifiques obtenues par amplification RAPD d' ADN genomique ou par amplification d' ADNr. Apres leur validation, les son des ont pu etre mises en ceuvre S354 A.-M. Davila et al.dans differentes applications. Elles ont ete utilisees pour cribler 400 souches de levures isolees de differents from ages fran«ais. Les souches ayant ete prealablement identifiees par des tests classiques, nous avons pu comparer les deux types de methodes d'identification, moleculaire et conventionnelle. La son de specifique de D. hansenii a He testee avec succes dans des essais d'hybridation sur colonie. Au total, ces son des se sont revelees etre de bons outils pour le suivi et l'inventaire de la biodiversite des levures fromageres. levures / fromage / identification moleculaire / hybridation / biodiversite

show abstract

Contribution à l'étude de l'origine des levures du fromage de Camembert

Abstract: Résumé

Cited by 44 publications

References 22 publications

DNA probes specific for the yeast species Debaryomyces hansenii: useful tools for rapid identification

DNA probes specific for the yeast species Debaryomyces hansenii: useful tools for rapid identification

DNA probes specific for the yeast speciesDebaryomyces hansenii: useful tools for rapid identification

Use of specific DNA probes for the rapid characterization of yeasts isolated from complex biotopes

Contact Info

Product

Resources

About