Contrasts between mucormycosis and aspergillosis in oncohematological patients

Климко, Н Н; Хостелиди, С Н; Шадривова, О В; Волкова, А. Г.; Popova, M.; Uspenskaya, Olga S.; Shneyder, Tatyana; Богомолова, Т С; Ignatyeva, S.; Zubarovskaya, Ludmila; Afanasyev, Boris

doi:10.1093/mmy/myy116

Cited by 32 publications

(27 citation statements)

References 30 publications

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“…Among the examined patients, adults predominated (n = 110; 95%), there were significantly fewer children (n = 6; 5%). Meanwhile, in our register, children and adolescents accounted for 36% of all patients with mucormycosis [9]. Analysis of information on the treatment of background hematologic disease showed that approximately the same number of patients after cytostatic therapy and allo-HSCT were included in the studies, which coincides with the data from our register [9].…”

Section: Resultssupporting

confidence: 79%

“…An increasing incidence of mucormycosis in hematological patients can be associated with the intensification of cytostatic chemotherapy and with the use of inactive against mucormycetes voriconazole for prevention and treatment of aspergillosis [6][7][8]. According to our previous studies [9], mucormycosis was diagnosed in 52% of patients within 1-225 days after the development of invasive aspergillosis. Meanwhile voriconazole was used in 74% of these patients for the treatment of aspergillosis.…”

Section: Introductionmentioning

confidence: 99%

“…According to this the role of early diagnosis of mucormycosis is growing, but the disease is often detected late, often postmortem [6,9,10]. The clinical and radiological signs are similar to other mold mycoses, for example aspergillosis [11].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review

Шадривова

Burygina

Климко

2019

JoF

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Objectives: to analyze the results of molecular methods applying for the diagnosis of mucormycosis in hematologic patients based on a literature review. Data sources: A systematic search in databases PubMed, Google Scholar for August 2019. Review eligibility criteria: original articles published in English, studies of molecular methods for the diagnosis of mucormycosis in hematologic patients. Results. We analyzed the research data from 116 hematological patients with mucormycosis, including children (6%). Patients with localized forms of mucormycosis prevailed (72%), and lung involvement was diagnosed in 58% of these cases. For molecular verification of the causative agent of mucormycosis, blood serum was most often used, less commonly postoperative and autopsy material, biopsy specimens, formalin-fixed paraffin-embedded samples and bronchoalveolar lavage, pleural fluid and sputum. The sensitivity of molecular diagnostics of mucormycosis in a cohort of hematological patients was 88.2%. Conclusion. The use of molecular techniques along with standard mycological methods will improve the diagnostics of mucormycosis in hematologic patients. However, prospective studies of the effectiveness of molecular methods for the diagnosis of mucormycosis of various etiologies in hematological patients, including children, using bronchoalveolar lavage (BAL) and cerebrospinal fluid (CSF) are needed.

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Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review

Шадривова

Burygina

Климко

2019

JoF

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“…Patients suffering from mucormycosis exhibit signs and symptoms similar to patients suffering from other invasive fungal infections, such as aspergillosis [26,27]. The ability to diagnose mucormycosis separately from other fungal infections is essential for therapeutic management and to improve patient outcomes.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Novel Mitochondrial Pan-Mucorales Marker for the Detection, Identification, Quantification, and Growth Stage Determination of Mucormycetes

Caramalho

Madl

Rosam

et al. 2019

JoF

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Mucormycosis infections are infrequent yet aggressive and serious fungal infections. Early diagnosis of mucormycosis and its discrimination from other fungal infections is required for targeted treatment and more favorable patient outcomes. The majority of the molecular assays use 18 S rDNA. In the current study, we aimed to explore the potential of the mitochondrial rnl (encoding for large-subunit-ribosomal-RNA) gene as a novel molecular marker suitable for research and diagnostics. Rnl was evaluated as a marker for: (1) the Mucorales family, (2) species identification (Rhizopus arrhizus, R. microsporus, Mucor circinelloides, and Lichtheimia species complexes), (3) growth stage, and (4) quantification. Sensitivity, specificity, discriminatory power, the limit of detection (LoD), and cross-reactivity were evaluated. Assays were tested using pure cultures, spiked clinical samples, murine organs, and human paraffin-embedded-tissue (FFPE) samples. Mitochondrial markers were found to be superior to nuclear markers for degraded samples. Rnl outperformed the UMD universal ® (Molyzm) marker in FFPE (71.5% positive samples versus 50%). Spiked blood samples highlighted the potential of rnl as a pan-Mucorales screening test. Fungal burden was reproducibly quantified in murine organs using standard curves. Identification of pure cultures gave a perfect (100%) correlation with the detected internal transcribed spacer (ITS) sequence. In conclusion, mitochondrial genes, such as rnl, provide an alternative to the nuclear 18 S rDNA genes and deserve further evaluation.

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“…Найчастіше блискавичний перебіг захворювання спостерігають за мукормікозу [6]. Більшість авторів описують плісняві мікози, як вторинну інфекцію при імунодефіцитних станах (ВІЛ/СНІД, при імуносупресії для проведення трансплантацій органів), онкологічних захворюваннях [7,8,9,10]. Osmanov&Denning (2015) на основі аналізу статистичних даних повідомляють, що в Україні досить загрозливим є зростання кількості інфекцій грибкової етіології, в тому числі аспергільозу і мукормікозу, на фоні ВІЛ/СНІД, туберкульозу, бронхіальної астми та онкологічних захворювань.…”

Section: вступunclassified

Cultural Properties of Fungi of the Family Mucoraceae, the Genus Aspergillus as Causative Agents of Zooanthroponosis

Кінаш¹,

Skotarenko²

2019

Act. Probl. of the Modern Med.

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At present, in Ukraine there is a growing prevalence of fungal infections, including aspergillosis and mucormycosis, against HIV / AIDS, tuberculosis, bronchial asthma and cancerous diseases [11]. The literature available presents scanty data on which of the most commonly used nutrient media provide the highest biosynthetic activity for the fungi of the family Mucoraceae, the genus Aspergillus. The aim of this study was to investigate the cultural properties of the studied isolates of fungi on different nutrient media. The following field fungi isolates were studied: Mucor ramosissimus Samutsevitsch, Rhizopus spp., Aspergillus fumigatus Fresenius, Aspergillus niger van Tieghem, Aspergillus flavus Link. The following nutrient media were used: the Plout medium, the Grigoraki medium, the Czapek agar, the Sabouraud agar, the wort-agar, the Van Eterson medium, the Sabouraud-dextrose broth, the honey medium. The concentration of fungi spores per 1 cm3 of inoculum was assessed on the basis of standard methods using a Goryaev's chamber. The evaluation of the intensity of spore formation on different nutrient media was carried out by standard techniques and assessed in CFU/ cm2 [30]. The most intense growth of micromycetes of the Mucor and Aspergillus genera is found on media containing sucrose, dextrose, maltose, glucose, dextrin, glycerol or plant components (the Sabouraud agar, the Czapek agar, the wort agar, the Grigoraki medium, and the Plout medium).

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Contrasts between mucormycosis and aspergillosis in oncohematological patients

Cited by 32 publications

References 30 publications

Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review

Molecular Diagnostics of Mucormycosis in Hematological Patients: A Literature Review

Evaluation of a Novel Mitochondrial Pan-Mucorales Marker for the Detection, Identification, Quantification, and Growth Stage Determination of Mucormycetes

Cultural Properties of Fungi of the Family Mucoraceae, the Genus Aspergillus as Causative Agents of Zooanthroponosis

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