Contrasting patterns of genetic diversity in neutral markers and agromorphological traits in wild and cultivated populations of Medicago sativa L. from Spain

Jenczewski, Eric; Angevain, M.; Charrier, André; Genier, G.; Ronfort, Joëlle; Prospéri, Jean-Marie

doi:10.1186/1297-9686-30-s1-s103

Cited by 10 publications

(18 citation statements)

References 25 publications

(11 reference statements)

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“…1). These populations were chosen in order to represent the three types of Spanish natural populations previously idengified using both nuclear markers and phenotypic data (Jenczewski et al . 1998, 1999a,b): (i) ‘intermediate’ natural populations, which contained a continuous range of plants with intermediate features between the wild and the cultivated forms and which are not significantly differentiated from the cultivated landraces based on molecular markers; (ii) ‘introgressed’ natural populations, displaying the typical wild morphology with few intermediate individuals, but not significantly differentiated from one cultivated Spanish landrace, Aragon , for neutral markers; and (iii) ‘wild type’ populations differentiated from the cultivated populations with respect to both quantitative traits and neutral markers (even if the genetic differentiation assessed with the molecular markers remains small).…”

Section: Methodsmentioning

confidence: 99%

“…A crawling growth habit and rhizomes, differing clearly from the cultivated erect phenotype, characterize typical wild plants (locally called ‘Mielga’). A previous study of the overall differentiation among natural and cultivated populations of M. sativa collected throughout Spain has suggested the occurrence of gene flow between the two entities (Jenczewski et al . 1998, 1999a,b).…”

Section: Introductionmentioning

confidence: 99%

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How mitochondrial DNA diversity can help to understand the dynamics of wild‐cultivated complexes. The case ofMedicago sativain Spain

et al. 2001

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In order to clarify the relationships (genetic exchange and shared ancestry) between natural and cultivated populations of alfalfa (Medicago sativa L.) in Spain, we investigated the patterns of mitochondrial DNA variation (characterized through restriction fragment length polymorphism) for 248 individuals in seven natural and six cultivated populations of this species. Mitochondrial variation was evidenced in both natural and cultivated populations of M. sativa. Among the seven mitotypes identified in the species, two were specific of the natural populations, a result attesting the fact that the Spanish wild form of M. sativa is an original genetic pool compared to the cultivated one. Other mitotypes were observed in both natural and cultivated populations, suggesting the occurrence of gene flow through seeds from cultivated towards natural populations. Comparisons with previously gathered nuclear and phenotypic data give insights into the different evolutionary forces acting on the different kinds of Spanish natural populations examined so far.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

How mitochondrial DNA diversity can help to understand the dynamics of wild‐cultivated complexes. The case ofMedicago sativain Spain

et al. 2001

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“…Wild plants display a crawling growth habit and rhizomes, distinguishing them from the domesticated erect phenotype (Prosperi et al . 1996; Jenczewski et al . 1998).…”

Section: Introductionmentioning

confidence: 99%

Domestication history in the Medicago sativa species complex: inferences from nuclear sequence polymorphism

et al. 2006

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DNA sequence polymorphism carries genealogical information and allows for testing hypotheses on selection and population history, especially through coalescent-based analysis. Understanding the evolutionary forces at work in plant domestication and subsequent selection is of critical importance for the management of genetic resources. In this study, we surveyed DNA sequence diversity at two assumed neutral nuclear loci in the wild-domesticated species complex of alfalfa (Medicago sativa L.). A high level of polymorphism was detected. The domesticated pool contains on average 31% less diversity than the wild pool, but with a high heterogeneity among loci. Coalescent simulations of the domestication process showed that this result cannot be explained by assuming a constant population size but is rather consistent with a demographic bottleneck during domestication. A very low level of divergence was detected between the wild and the domesticated forms as well as between the related subspecies of the M. sativa species complex. However, the originality of the Spanish wild populations, already observed based on mitochondrial DNA polymorphism, was confirmed. These results, together with patterns of intrapopulation polymorphism, suggest that nuclear sequence polymorphism could be a promising tool, complementary to mitochondrial DNA and phenotypic evaluations, to investigate historical demographic and evolutionary processes.

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“…A lack of strong correlation between phenotypic and molecular markers has been reported by other authors (e.g., Pissard et al 2008). Some studies have indicated the distinct phenotypic separation of wild or 'Mielga' alfalfa populations from alfalfa cultivars (Crochemore et al 1998;Jenczewski et al 1998;Julier et al 1995) but similar reports do not exist for feral alfalfa populations. The phenotypic variables we investigated were not sufficient for segregating feral populations from cultivars.…”

Section: Discussionmentioning

confidence: 86%

“…Feral alfalfa populations may facilitate novel trait movement and as such thorough investigations on the nature of these populations is warranted. Studies have been done to assess differences among natural and cultivated alfalfa populations (e.g., Jenczewski et al 1998). However, no study has yet established the genetic diversity of feral alfalfa populations occurring in roadside habitats in regions where alfalfa is cultivated in neighboring fields.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of feral alfalfa (Medicago sativa L.) populations occurring in Manitoba, Canada and comparison with alfalfa cultivars: an analysis using SSR markers and phenotypic traits

et al. 2010

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Feral populations of cultivated crops may act as reservoirs for novel traits and aid in trait movement across the landscape. Knowledge on the genetic diversity of feral populations may provide new insights into their origin and evolution and may help in the design of efficient novel trait confinement protocols. In this study, the genetic diversity of 12 feral alfalfa (Medicago sativa) populations originating from southern Manitoba (Canada) and 10 alfalfa cultivars and a M. falcata germplasm were investigated using eight SSR markers (i.e., microsatellites) and 14 phenotypic traits. We found that the genetic diversity observed in feral populations was similar to the diversity detected among the 10 cultivars. Analysis of molecular variance revealed that there was great genetic variation within (99.8%) rather than between different feral populations. Cluster analysis (unweighted pair-group method using arithmetic average) revealed no differentiation between feral populations and cultivars for neutral loci. High levels of population differentiation for phenotypic traits (and not for neutral markers) suggest the occurrence of heterogeneous selection for adaptive traits. The phenotypic traits we studied did not distinctly separate feral populations from cultivars but there was evidence of natural selection in feral populations for traits including winter survivability, rhizome production, and prostrate growth habit. Our results suggest that feral alfalfa populations need to be considered in the risk assessment of alfalfa containing novel genetically modified (GM) traits. Further, feral alfalfa populations may be regarded as a source of new germplasm for plant improvement.

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Contrasting patterns of genetic diversity in neutral markers and agromorphological traits in wild and cultivated populations of Medicago sativa L. from Spain

Cited by 10 publications

References 25 publications

How mitochondrial DNA diversity can help to understand the dynamics of wild‐cultivated complexes. The case ofMedicago sativain Spain

How mitochondrial DNA diversity can help to understand the dynamics of wild‐cultivated complexes. The case ofMedicago sativain Spain

Domestication history in the Medicago sativa species complex: inferences from nuclear sequence polymorphism

Genetic diversity of feral alfalfa (Medicago sativa L.) populations occurring in Manitoba, Canada and comparison with alfalfa cultivars: an analysis using SSR markers and phenotypic traits

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