Contrasting patterns of gene expression indicate differing pyrethroid resistance mechanisms across the range of the New World malaria vector Anopheles albimanus

Mackenzie-Impoinvil, Lucy; Weedall, Gareth D.; Lol, Juan C.; Pinto, Jesús; Vizcaino, Lucrecia; Dzuris, Nicole; Riveron, Jacob M.; Padilla, Norma; Wondji, Charles S.; Lenhart, Audrey

doi:10.1371/journal.pone.0210586

Cited by 25 publications

(17 citation statements)

References 57 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recent studies using whole transcriptome sequencing have shown that the cytochrome P450 CYP9K1 was overexpressed in deltamethrin and alpha-cypermethrin-resistant samples from Peru and in deltamethrin-resistant samples from Guatemala and CYP6P5 overexpression in deltamethrin-resistant samples from Peru. In that same study, kdr mutations were also detected at the L1014S/C position in deltamethrin and alpha-cypermethrin-resistant samples from Peru [40]. Additionally, studies of the microbiota in mosquitoes of differing insecticide resistance status have showed differing composition of the microbiota and its functions between fenitrothion-susceptible and fenitrothion-resistance mosquitoes from Peru [41].…”

Section: Discussionmentioning

confidence: 99%

Absence of knockdown mutations in pyrethroid and DDT resistant populations of the main malaria vectors in Colombia

Orjuela

Álvarez-Díaz

Morales

et al. 2019

Malar J

View full text Add to dashboard Cite

BackgroundKnockdown resistance (kdr) is a well-characterized target-site insecticide resistance mechanism that is associated with DDT and pyrethroid resistance. Even though insecticide resistance to pyrethroids and DDT have been reported in Anopheles albimanus, Anopheles benarrochi sensu lato (s.l.), Anopheles darlingi, Anopheles nuneztovari s.l., and Anopheles pseudopunctipennis s.l. malaria vectors in Latin America, there is a knowledge gap on the role that kdr resistance mechanisms play in this resistance. The aim of this study was to establish the role that kdr mechanisms play in pyrethroid and DDT resistance in the main malaria vectors in Colombia, in addition to previously reported metabolic resistance mechanisms, such as mixed function oxidases (MFO) and nonspecific esterases (NSE) enzyme families.MethodsSurviving (n = 62) and dead (n = 67) An. nuneztovari s.l., An. darlingi and An. albimanus mosquitoes exposed to diagnostic concentrations of DDT and pyrethroid insecticides were used to amplify and sequence a ~ 225 bp fragment of the voltage-gated sodium channels (VGSC) gene. This fragment spanning codons 1010, 1013 and 1014 at the S6 segment of domain II to identify point mutations, which have been associated with insecticide resistance in different species of Anopheles malaria vectors.ResultsNo kdr mutations were detected in the coding sequence of this fragment in 129 samples, 62 surviving mosquitoes and 67 dead mosquitoes, of An. darlingi, An. nuneztovari s.l. and An. albimanus.ConclusionMutations in the VGSC gene, most frequently reported in other species of the genus Anopheles resistant to pyrethroid and DDT, are not associated with the low-intensity resistance detected to these insecticides in some populations of the main malaria vectors in Colombia. These results suggest that metabolic resistance mechanisms previously reported in these populations might be responsible for the resistance observed.

show abstract

Section: Discussionmentioning

confidence: 99%

Absence of knockdown mutations in pyrethroid and DDT resistant populations of the main malaria vectors in Colombia

Orjuela

Álvarez-Díaz

Morales

et al. 2019

Malar J

View full text Add to dashboard Cite

show abstract

“…Changes in intracellular proteases in insects subjected to insecticidal stresses have relevant implications for the evolution of insecticide resistance and the management of insecticide‐resistant strains (Gao et al, 2020; Mackenzie‐Impoinvil et al, 2019; Wilkins, 2017). Here, for the first time, we characterised the proteolytic activities in the salivary glands of phytosuccivorous stink bug pests with different levels of insecticide susceptibilities.…”

Section: Discussionmentioning

confidence: 99%

Proteolytic enzymes in the salivary glands of the Neotropical brown stink bugEuschistus heros: Reduced activities in imidacloprid‐resistant strains

Campos

Meriño‐Cabrera

Mantilla‐Afanador

et al. 2021

Annals of Applied Biology

View full text Add to dashboard Cite

Proteases present in the salivary glands of phytosuccivorous insects are responsible for the initial breakdown of dietary protein into the amino acids necessary for insect growth and development. Here, we characterised the total proteases and trypsin‐like activities in the salivary glands of the Neotropical brown stink bug, Euschistus heros (F.) and used in silico and biochemical approaches to determine the interactions between trypsin‐like enzymes and the soybean Kunitz trypsin inhibitor (SKTI). Additionally, we evaluated whether these enzyme activities were affected in individuals resistant to the actions of the insecticide imidacloprid. We used an originally field‐collected strain to select individuals resistant to imidacloprid alone (ImiGoiasRes) or synergised with piperonyl butoxide (ImiPBORes). A laboratory‐susceptible (ImiSusc) strain was also used to select piperonyl butoxide synergised imidacloprid‐resistant (ImiPBOSel) individuals. Our results revealed that pH and temperature significantly affected the specific trypsin‐like activities in imidacloprid‐susceptible individuals. Significant alterations in the total protease activities were recorded for field‐ (ImiGoiasRes) and laboratory‐selected imidacloprid‐resistant individuals (ImiPBOSel and ImiPBORes). Our computational docking analysis demonstrated that the molecular interactions and the presence of the SKTI inhibitor (0.5 mg mL−1) could reduce up to 40% of the trypsin‐like activities of imidacloprid‐susceptible individuals. However, all imidacloprid‐resistant strains exhibited significantly less trypsin‐like activities and exhibited lower hydrolysing velocity (VMAX) and affinity (KM) rates, and the prior exposure to SKTI only synergised the imidacloprid toxicity for individuals of ImiFieldRes strain. Collectively, our findings demonstrate, for the first time, insecticide resistance detrimentally affected the activities of proteolytic enzymes in phytosuccivorous stink bug pests.

show abstract

“…sinensis [ 72 , 265 ] CYP4C26, CYP6P5, CYP9K1 in An. albimanus [ 266 ] Carbamates AChE-G119S in An. gambiae , An.…”

Section: Metabolic Proteins and Insecticide Resistancementioning

confidence: 99%

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

et al. 2020

View full text Add to dashboard Cite

The increasing resistance to currently available insecticides in the malaria vector, Anopheles mosquitoes, hampers their use as an effective vector control strategy for the prevention of malaria transmission. Therefore, there is need for new insecticides and/or alternative vector control strategies, the development of which relies on the identification of possible targets in Anopheles. Some known and promising targets for the prevention or control of malaria transmission exist among Anopheles metabolic proteins. This review aims to elucidate the current and potential contribution of Anopheles metabolic proteins to malaria transmission and control. Highlighted are the roles of metabolic proteins as insecticide targets, in blood digestion and immune response as well as their contribution to insecticide resistance and Plasmodium parasite development. Furthermore, strategies by which these metabolic proteins can be utilized for vector control are described. Inhibitors of Anopheles metabolic proteins that are designed based on target specificity can yield insecticides with no significant toxicity to non-target species. These metabolic modulators combined with each other or with synergists, sterilants, and transmission-blocking agents in a single product, can yield potent malaria intervention strategies. These combinations can provide multiple means of controlling the vector. Also, they can help to slow down the development of insecticide resistance. Moreover, some metabolic proteins can be modulated for mosquito population replacement or suppression strategies, which will significantly help to curb malaria transmission.

show abstract

Contrasting patterns of gene expression indicate differing pyrethroid resistance mechanisms across the range of the New World malaria vector Anopheles albimanus

Cited by 25 publications

References 57 publications

Absence of knockdown mutations in pyrethroid and DDT resistant populations of the main malaria vectors in Colombia

Absence of knockdown mutations in pyrethroid and DDT resistant populations of the main malaria vectors in Colombia

Proteolytic enzymes in the salivary glands of the Neotropical brown stink bugEuschistus heros: Reduced activities in imidacloprid‐resistant strains

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

Contact Info

Product

Resources

About