Contrasting increased levels of serum amyloid A in patients with three different bone sarcomas: An indicator of tumor malignancy?

Wan-Ibrahim, Wan Izlina; Ashrafzadeh, Ali; Singh, Vivek Ajit; Hashim, Onn Haji; Abdul‐Rahman, Puteri Shafinaz

doi:10.1002/elps.201500522

Cited by 2 publications

(2 citation statements)

References 37 publications

(48 reference statements)

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“…These protein spots were confirmed of their identities using mass spectrometry analysis (MALDI‐MS/MS) as described in Wan‐Ibrahim et al. [17].…”

Section: Methodsmentioning

confidence: 99%

“…The presence of silverstained protein spots also allowed visual comparison with the lectin-generated blots. These protein spots were confirmed of their identities using mass spectrometry analysis (MALDI-MS/MS) as described in Wan-Ibrahim et al [17]. The electrophoretic transfer of gels onto nitrocellulose membranes was performed for 2 h at a constant current of 0.8 mM/cm 2 using NovaBlot Kit.…”

Section: Detection Of Milk Serum Glycoproteins Using Lectin-blotting mentioning

confidence: 99%

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Isolation and identification of O‐ and N‐linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth

et al. 2020

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Milk serves as the sole nutrition for newborns, as well as a medium for the transfer of immunological components from the mother to the baby. This study reveals different glycoprotein profiles obtained from human, bovine, and caprine milk and their potential roles in supporting infant growth. Proteins from these three milk samples are separated and analyzed using two‐dimensional gel electrophoresis (2‐DE). Glycosylated proteins from all samples are enriched by affinity chromatography using lectins from the seeds of Artocarpus integer before analysis using LC/MS‐QTOF. The glycoproteome profiling demonstrates that glycosylated proteins are higher in caprine milk compared to other samples. Analysis using LC/MS‐QTOF identified 42 O‐glycosylated and 56 N‐glycosylated proteins, respectively. Among those identified, human milk has 17 glycoproteins, which are both O‐ and N‐glycosylated, whereas caprine and bovine have 10 and 1, respectively. Only glycoproteins from human milk have shown positive matching to important human biological pathways, such as vesicle‐mediated transport, immune system and hemostasis pathways. Human milk remains unique for human babies with the presence of antibodies in the form of immunoglobulins that are lacking in ruminant milk proteomes.

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“…These protein spots were confirmed of their identities using mass spectrometry analysis (MALDI‐MS/MS) as described in Wan‐Ibrahim et al. [17].…”

Section: Methodsmentioning

confidence: 99%

Section: Detection Of Milk Serum Glycoproteins Using Lectin-blotting mentioning

confidence: 99%

Isolation and identification of O‐ and N‐linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth

et al. 2020

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Utility of a Simple Scoring System in Differentiating Bacterial Infections in Cases of Fever of Unknown Origin

Wang

Shi

et al. 2020

Clinical Infectious Diseases

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Background Infectious disease is the leading cause of fever of unknown origin (FUO). Serum inflammatory markers historically used to diagnose bacterial infection have sufficient diagnostic sensitivity but low specificity. This study aimed to develop a simple scoring system for differentiating bacterial infections from other causes of early-stage FUO. Methods This study included a retrospective cohort of patients presenting with FUO at the Huashan Hospital (January 2014 to June 2017). The diagnostic utility of serum inflammatory markers for bacterial infection was evaluated using the receiver operating characteristic (ROC) curve analysis. Relevant markers were subsequently measured prospectively in a separate cohort of FUO patients (December 2017 to May 2019). A scoring system was based on inflammatory markers and other test results. Results Bacterial infection was identified in 34% of patients in the retrospective cohort. The area under the ROC curve (AUC) was 0.644 (95% confidence interval [CI], .595–.693) for C-reactive protein, 0.624 (95% CI, .573–.675) for procalcitonin, and 0.646 (95% CI, .595–.697) for serum ferritin (SF) in diagnosing bacterial infection. Bacterial infection was found in 29% of cases in the prospective cohort. A model based on serum amyloid A (SAA) and SF levels and neutrophil percentage yielded an AUC of 0.775 (95% CI, .695–.854). Validation analysis indicated lower probability (<15%) of bacterial infection for patients with a score <16.5 points. Conclusions A scoring system based on SAA and SF levels and neutrophil percentage can help distinguish bacterial infection from other causes of FUO, potentially reducing antibiotic use.

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Contrasting increased levels of serum amyloid A in patients with three different bone sarcomas: An indicator of tumor malignancy?

Cited by 2 publications

References 37 publications

Isolation and identification of O‐ and N‐linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth

Isolation and identification of O‐ and N‐linked glycoproteins in milk from different mammalian species and their roles in biological pathways which support infant growth

Utility of a Simple Scoring System in Differentiating Bacterial Infections in Cases of Fever of Unknown Origin

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