Contrasting genetic structure in Plasmodium vivax populations from Asia and South America

Imwong, Mallika; Nair, Shalini; Pukrittayakamee, Sasithon; Sudimack, Daniel; Williams, Jeff T.; Mayxay, Mayfong; Newton, Paul N.; Kim, Jung Ryong; Nandy, Amitab; Osorio, Lyda; Carlton, Jane M.; White, Nicholas J.; Day, Nicholas P. J.; Anderson, Tim

doi:10.1016/j.ijpara.2007.02.010

Cited by 149 publications

(248 citation statements)

References 35 publications

Supporting

Mentioning

215

Contrasting

Unclassified

Order By: Relevance

“…In addition, it was initially suggested that there was little polymorphic variation at these microsatellites among worldwide isolates (Leclerc et al 2004). However, highly variable microsatellite markers have been more recently described and used in population-level studies of genetic variation in this species (Gomez et al 2003, 2007a, b, Ferreira et al 2007.…”

Section: Varying Detection Methodsmentioning

confidence: 99%

“…Stutter peaks result from DNA strand slippage during PCR at intervals corresponding to nucleotide repeat sizes (de Valk et al 2007). A minimum value of either one-third (Anderson et al 2000, Imwong et al 2007a, Karunaweera et al 2008 or one-fourth (Anderson et al 1999) of the predominant allele peak height has been used as a cut-off to differentiate minor alleles from stutter or unspecific peaks, but the sensitivity and specificity of these criteria remain undetermined (Greenhouse et al 2006, Havryliuk et al 2008.…”

Section: Varying Detection Methodsmentioning

confidence: 99%

“…These strict criteria have been suggested because the detection of two or more alleles at some microsatellite loci may be a result of any of the artefacts mentioned above, which could potentially lead to an overestimation of the proportion of multiple-clone infections. Imwong et al (2007a) observed great drops in multiple-clone infection detection rates in P. vivax isolates from South America and Asia as the requirement for the number of microsatellite loci with multiple alleles Fig. 1: electropherograms for two microsatellite markers, amplified from the same mixture of genomic DNA from two Plasmodium vivax clones (here identified as clone 1 and clone 2).…”

Section: Varying Detection Methodsmentioning

confidence: 99%

See 2 more Smart Citations

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

Havryliuk

Ferreira

2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Section: Varying Detection Methodsmentioning

confidence: 99%

Section: Varying Detection Methodsmentioning

confidence: 99%

Section: Varying Detection Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

Havryliuk

Ferreira

2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

“…Nevertheless, substantial divergence was seen between subpopulations in South America [8]. Putatively neutral microsatellite markers, sampled from across the genome [9], have recently been used to characterize field populations of P. vivax [10-12], suggesting that a spectrum of population structures also exists for this species. Again, South American parasite populations sampled from nearby sites are highly divergent [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Putatively neutral microsatellite markers, sampled from across the genome [9], have recently been used to characterize field populations of P. vivax [10-12], suggesting that a spectrum of population structures also exists for this species. Again, South American parasite populations sampled from nearby sites are highly divergent [10,11]. However, because the microsatellite markers analyzed map to different chromosomes, prior studies could not examine chromosome-level LD and infer recombination rates in P. vivax .…”

Section: Introductionmentioning

confidence: 99%

Single-nucleotide polymorphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax: prospects for genome-wide association studies

et al. 2010

View full text Add to dashboard Cite

BackgroundThe ideal malaria parasite populations for initial mapping of genomic regions contributing to phenotypes such as drug resistance and virulence, through genome-wide association studies, are those with high genetic diversity, allowing for numerous informative markers, and rare meiotic recombination, allowing for strong linkage disequilibrium (LD) between markers and phenotype-determining loci. However, levels of genetic diversity and LD in field populations of the major human malaria parasite P. vivax remain little characterized.ResultsWe examined single-nucleotide polymorphisms (SNPs) and LD patterns across a 100-kb chromosome segment of P. vivax in 238 field isolates from areas of low to moderate malaria endemicity in South America and Asia, where LD tends to be more extensive than in holoendemic populations, and in two monkey-adapted strains (Salvador-I, from El Salvador, and Belem, from Brazil). We found varying levels of SNP diversity and LD across populations, with the highest diversity and strongest LD in the area of lowest malaria transmission. We found several clusters of contiguous markers with rare meiotic recombination and characterized a relatively conserved haplotype structure among populations, suggesting the existence of recombination hotspots in the genome region analyzed. Both silent and nonsynonymous SNPs revealed substantial between-population differentiation, which accounted for ~40% of the overall genetic diversity observed. Although parasites clustered according to their continental origin, we found evidence for substructure within the Brazilian population of P. vivax. We also explored between-population differentiation patterns revealed by loci putatively affected by natural selection and found marked geographic variation in frequencies of nucleotide substitutions at the pvmdr-1 locus, putatively associated with drug resistance.ConclusionThese findings support the feasibility of genome-wide association studies in carefully selected populations of P. vivax, using relatively low densities of markers, but underscore the risk of false positives caused by population structure at both local and regional levels.See commentary: http://www.biomedcentral.com/1741-7007/8/90

show abstract

Primaquine for preventing relapse in people withPlasmodium vivaxmalaria treated with chloroquine

Galappaththy¹,

Tharyan

Kirubakaran

2013

Cochrane Database of Systematic Reviews

View full text Add to dashboard Cite

The analysis confirms the current World Health Organization recommendation for 14-day primaquine (15 mg/day) to prevent relapse of vivax malaria. Shorter primaquine regimens at the same daily dose are associated with higher relapse rates. The comparative effects with weekly primaquine are promising, but require further trials to establish equivalence or non-inferiority compared to the 14-day regimen in high malaria transmission settings.

show abstract

Contrasting genetic structure in Plasmodium vivax populations from Asia and South America

Cited by 149 publications

References 35 publications

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

A closer look at multiple-clone Plasmodium vivax infections: detection methods, prevalence and consequences

Single-nucleotide polymorphism, linkage disequilibrium and geographic structure in the malaria parasite Plasmodium vivax: prospects for genome-wide association studies

Primaquine for preventing relapse in people withPlasmodium vivaxmalaria treated with chloroquine

Contact Info

Product

Resources

About