Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: implications for vector control

Rašić, Gordana; Schama, Renata; Powell, Rosanna; Maciel-de-Freitas, Rafael; Endersby‐Harshman, Nancy M.; Filipović, Igor; Sylvestre, Gabriel; Maspero, Renato; Hoffmann, Ary A.

doi:10.1111/eva.12301

Cited by 37 publications

(31 citation statements)

References 99 publications

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“…This enzyme combination has also been used to construct ddRADseq libraries in Ae. aegypti [29][30][31]41].…”

Section: Snp Discoverymentioning

confidence: 99%

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Genome-Wide SNPs Reveal the Drivers of Gene Flow In An Urban Population of the Asian Tiger Mosquito,Aedes albopictus

Schmidt

Rašić

Zhang³

et al. 2017

Preprint

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Aedes albopictus is a highly invasive disease vector with an expanding worldwide distribution. Genetic assays using low to medium resolution markers have found little evidence of spatial genetic structure even at broad geographic scales, suggesting frequent passive movement along human transportation networks. Here we analysed genetic structure of Ae. albopictus collected from 12 sample sites in Guangzhou, China, using thousands of genome-wide single nucleotide polymorphisms (SNPs). We found evidence for passive gene flow, with distance from shipping terminals being the strongest predictor of genetic distance among mosquitoes. As further evidence of passive dispersal, we found multiple pairs of full-siblings distributed between two sample sites 3.7 km apart. After accounting for geographical variability, we also found evidence for isolation by distance, previously undetectable in Ae. albopictus. These findings demonstrate how large SNP datasets and spatially-explicit hypothesis testing can be used to decipher processes at finer geographic scales than formerly possible. Our approach can be used to help predict new invasion pathways of Ae. albopictus and to refine strategies for vector control that involve the transformation or suppression of mosquito populations.. CC-BY-NC4.0 International license not peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint ( Author SummaryAedes albopictus, the Asian Tiger Mosquito, is a highly invasive disease vector with a growing global distribution. Designing strategies to prevent invasion and to control Ae. albopictus populations in invaded regions requires knowledge of how Ae. albopictus disperses. Studies comparing Ae. albopictus populations have found little evidence of genetic structure even between distant populations, suggesting that dispersal along human transportation networks is common. However, a more specific understanding of dispersal processes has been unavailable due to an absence of studies using high-resolution genetic markers. Here we present a study using high-resolution markers, which investigates genetic structure among 152 Ae. albopictus from Guangzhou, China. We found that human transportation networks, particularly shipping terminals, had an influence on genetic structure. We also found genetic distance was correlated with geographical distance, the first such observation in this species. This study demonstrates how high-resolution markers can be used to investigate ecological processes that may otherwise escape detection. We conclude that strategies for controlling Ae. albopictus will have to consider both passive reinvasion along human transportation networks and active reinvasion from neighbouring regions.

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“…This enzyme combination has also been used to construct ddRADseq libraries in Ae. aegypti [29][30][31]41].…”

Section: Snp Discoverymentioning

confidence: 99%

“…albopictus samples within countries [17,18] and cities [26], structure at these scales is regularly observed in Ae. aegypti, the primary Dengue vector [27][28][29][30][31]. This seems incongruous given that the estimates of the flight range potential in Ae.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide SNPs Reveal the Drivers of Gene Flow In An Urban Population of the Asian Tiger Mosquito,Aedes albopictus

Schmidt

Rašić

Zhang³

et al. 2017

Preprint

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“…pl program that runs the Stacks v.1.35 pipeline [26]. In addition to the samples from Singapore, Townsville and Cairns, we included previously sequenced individuals: 15 from Rio de Janeiro (Brazil) [27], 15 from Gordonvale (northern Queensland), and 15 from Ho Chi Minh City (Vietnam) (S1 Table). This was done to compare the extent of genetic structuring within and among samples at a regional and global scale.…”

Section: Methodsmentioning

confidence: 99%

The queenslandensis and the type Form of the Dengue Fever Mosquito (Aedes aegypti L.) Are Genomically Indistinguishable

et al. 2016

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BackgroundThe mosquito Aedes aegypti (L.) is a major vector of viral diseases like dengue fever, Zika and chikungunya. Aedes aegypti exhibits high morphological and behavioral variation, some of which is thought to be of epidemiological significance. Globally distributed domestic Ae. aegypti have often been grouped into (i) the very pale variety queenslandensis and (ii) the type form. Because the two color forms co-occur across most of their range, there is interest in understanding how freely they interbreed. This knowledge is particularly important for control strategies that rely on mating compatibilities between the release and target mosquitoes, such as Wolbachia releases and SIT. To address this question, we analyzed nuclear and mitochondrial genome-wide variation in the co-occurring pale and type Ae. aegypti from northern Queensland (Australia) and Singapore.Methods/FindingsWe typed 74 individuals at a 1170 bp-long mitochondrial sequence and at 16,569 nuclear SNPs using a customized double-digest RAD sequencing. 11/29 genotyped individuals from Singapore and 11/45 from Queensland were identified as var. queenslandensis based on the diagnostic scaling patterns. We found 24 different mitochondrial haplotypes, seven of which were shared between the two forms. Multivariate genetic clustering based on nuclear SNPs corresponded to individuals’ geographic location, not their color. Several family groups consisted of both forms and three queenslandensis individuals were Wolbachia infected, indicating previous breeding with the type form which has been used to introduce Wolbachia into Ae. aegypti populations.ConclusionAedes aegypti queenslandensis are genomically indistinguishable from the type form, which points to these forms freely interbreeding at least in Australia and Singapore. Based on our findings, it is unlikely that the presence of very pale Ae. aegypti will affect the success of Aedes control programs based on Wolbachia-infected, sterile or RIDL mosquitoes.

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“…Adults were identified as males or females based on the sexually dimorphic antennae and external genitalia structure (Becker 2003). Mosquitoes from Rio de Janeiro, Brazil were collected from ovitraps in November-December 2011 (Rasic, et al 2015). Larvae were reared until the third instar in an insectary under controlled conditions (25± 1°C, 80±10% relative humidity and 12:12 hour light-dark cycle).…”

Section: Methodsmentioning

confidence: 99%

Extensive genetic differentiation between homomorphic sex chromosomes in the mosquito vector, Aedes aegypti

Fontaine

Filipović

Fansiri

et al. 2016

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26Mechanisms and evolutionary dynamics of sex-determination systems are of 27 particular interest in insect vectors of human pathogens like mosquitoes because novel 28 control strategies aim to convert pathogen-transmitting females into non-biting males, or rely 29 on accurate sexing for the release of sterile males. In Aedes aegypti, the main vector of 30 dengue and Zika viruses, sex determination is governed by a dominant male-determining 31 locus, previously thought to reside within a small, non-recombining, sex-determining region 32 (SDR) of an otherwise homomorphic sex chromosome. Here, we provide evidence that sex 33 chromosomes in Ae. aegypti are genetically differentiated between males and females over a 34 region much larger than the SDR. Our linkage mapping intercrosses failed to detect 35 recombination between X and Y chromosomes over a 123-Mbp region (40% of their physical 36 length) containing the SDR. This region of reduced male recombination overlapped with a 37 smaller 63-Mbp region (20% of the physical length of the sex chromosomes) displaying high 38 male-female genetic differentiation in unrelated wild population from Brazil and Australia 39and in a reference laboratory strain originating from Africa. In addition, the sex-differentiated 40 genomic region was associated with a significant excess of male-to-female heterozygosity 41 and contained a small cluster of loci consistent with Y-specific null alleles. We demonstrate 42 that genetic differentiation between sex chromosomes is sufficient to assign individuals to 43 their correct sex with high accuracy. We also show how data on allele frequency differences 44 between sexes can be used to estimate linkage disequilibrium between loci and the sex-45 determining locus. Our discovery of large-scale genetic differentiation between sex 46 chromosomes in Ae. aegypti lays a new foundation for mapping and population genomic 47 studies, as well as for mosquito control strategies targeting the sex-determination pathway. 48 49 50 51 Understanding the underlying mechanisms and evolutionary dynamics of sex 52 determination in mosquitoes is of particular interest as new strategies for controlling 53 mosquito-borne diseases aim to convert pathogen-transmitting females into non-biting males 54 (Hall, et al. 2015), or rely on accurate sexing for the release of sterile males (Eckermann, et 55 al. 2014; Gilles, et al. 2014). Sex determination in mosquitoes and other dipterans is under 56 the control of a gene regulation cascade that relies on alternative splicing of genes expressed 57 in both males and females (Salz 2011). There is a great variation across dipteran species, and 58 even between populations of the same species, in how this cascade is initiated (Bopp, et al. 59 2014). The master switch at the top of the cascade in drosophilids is the number of X 60 chromosomes, whereas in tephritids, houseflies and mosquitoes it is a dominant male-61 determining factor (Kaiser and Bachtrog 2010; Vicoso and Bachtrog 2015). 62 In Aedes and Culex mosquitoes, the male-d...

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Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: implications for vector control

Cited by 37 publications

References 99 publications

Genome-Wide SNPs Reveal the Drivers of Gene Flow In An Urban Population of the Asian Tiger Mosquito,Aedes albopictus

Genome-Wide SNPs Reveal the Drivers of Gene Flow In An Urban Population of the Asian Tiger Mosquito,Aedes albopictus

The queenslandensis and the type Form of the Dengue Fever Mosquito (Aedes aegypti L.) Are Genomically Indistinguishable

Extensive genetic differentiation between homomorphic sex chromosomes in the mosquito vector, Aedes aegypti

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