Contrasting effects of siderophores pyoverdine and desferrioxamine B on the mobility of iron, aluminum, and copper in Cu-contaminated soils

Cornu, Jean-Yves; Gutiérrez, Margarita; Randriamamonjy, S.; Gaudin, Pierre; Ouédraogo, Frédéric; Sourzac, Mahaut; Parlanti, E.; Lebeau, Thierry; Janot, Noémie

doi:10.1016/j.geoderma.2022.115897

Cited by 7 publications

(2 citation statements)

References 48 publications

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“…The HPLC–MS and NMR data also show the presence of siderophores of the hydroxamate type, which are unique to the chemical desferrioxamine, and a similar finding was previously published [ 43 ]. Cornu et al [ 44 ] revealed that adding desferrioxamine B increased the mobility of Fe and Al in a wide range of soils and over a wide range of soil pH (5.9 < pH water < 8.6). The effect of desferrioxamine B was highly significant ( p < 0.001) for the concentrations of Fe.…”

Section: Discussionmentioning

confidence: 99%

Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts

Nithyapriya,

Sundaram,

Eswaran

et al. 2024

Microb Ecol

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Microorganisms produce siderophores, which are low-molecular-weight iron chelators when iron availability is limited. The present analyzed the role of LNPF1 as multifarious PGPR for improving growth parameters and nutrient content in peanut and soil nutrients. Such multifarious PGPR strains can be used as effective bioinoculants for peanut farming. In this work, rhizosphere bacteria from Zea mays and Arachis hypogaea plants in the Salem area of Tamil Nadu, India, were isolated and tested for biochemical attributes and characteristics that stimulate plant growth, such as the production of hydrogen cyanide, ammonia (6 µg/mL), indole acetic acid (76.35 µg/mL), and solubilizing phosphate (520 µg/mL). The 16S rRNA gene sequences identified the isolate LNPF1 as Pseudomonas fluorescens with a similarity percentage of 99% with Pseudomonas sp. Isolate LNPF1 was evaluated for the production of siderophore. Siderophore-rich supernatant using a Sep Pack C18 column and Amberlite-400 Resin Column (λmax 264) produced 298 mg/L and 50 mg/L of siderophore, respectively. The characterization of purified siderophore by TLC, HPLC, FTIR, and 2D-NMR analysis identified the compound as desferrioxamine, a hydroxamate siderophore. A pot culture experiment determined the potential of LNPF1 to improve iron and oil content and photosynthetic pigments in Arachis hypogaea L. and improve soil nutrient content. Inoculation of A. hypogea seeds with LNPF1 improved plant growth parameters such as leaf length (60%), shoot length (22%), root length (54.68%), fresh weight (47.28%), dry weight (37%), and number of nuts (66.66) compared to the control (untreated seeds). This inoculation also improved leaf iron content (43.42), short iron content (38.38%), seed iron (46.72%), seed oil (31.68%), carotenoid (64.40%), and total chlorophyll content (98.%) compared to control (untreated seeds). Bacterized seeds showed a substantial increase in nodulation (61.65%) and weight of individual nodules (95.97) vis-à-vis control. The results of the present study indicated that P. fluorescens might be utilized as a potential bioinoculant to improve growth, iron content, oil content, number of nuts and nodules of Arachishypogaea L., and enrich soil nutrients.

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Section: Discussionmentioning

confidence: 99%

Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts

Nithyapriya,

Sundaram,

Eswaran

et al. 2024

Microb Ecol

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“…Similarly, a loopful of the log-phase bacterial culture was inoculated into the succinate broth supplemented with ammonium nitrate, yeast extract, protease peptone, and potassium nitrate separately. The flasks were incubated at 37 • C for 48-72 h at 120 rpm, after which 1 ml of the culture filtrate was added to 1 ml of the CAS solution; the absorbance was measured at 630 nm; and %siderophore units were calculated (Cornu et al, 2022).…”

Section: E Ect Of Carbon and Nitrogen Sources On Siderophore Productionmentioning

confidence: 99%

Optimization and identification of siderophores produced by Pseudomonas monteilii strain MN759447 and its antagonism toward fungi associated with mortality in Dalbergia sissoo plantation forests

et al. 2022

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Siderophore-positive bacteria present in the rhizosphere and in bulk soil assist plants by either inhibiting phytopathogen proliferation or increasing plant growth. The bacterial diversity of the Shisham forest ecosystem in the Tarai region of the Western Himalayas was studied and used for siderophore production, taking into account the large-scale dieback and wilt-induced mortality in Dalbergia sissoo (common name: shisham) plantation forests and the importance of soil microbes in tree health. In addition, Pseudomonas, Burkholderia, and Streptomyces were prominent siderophore-positive bacteria in Shisham forests. Pseudomonas species are known for their remarkable siderophore-producing ability. Bacterial siderophores inhibit pathogen growth by rapidly lowering the number of ferric ions in the rhizosphere. The Pseudomonas monteilii strain MN759447 was isolated from a D. sissoo plantation forest at the Agroforestry Research Centre, Pantnagar, Uttarakhand (28°58′N 79°25′E/28.97°N 79.41°E). It produces a significant number of siderophore units (80.36% in total). A two-stage optimization of growth factors was attempted in the strain MN759447 for better siderophore recovery. In the first-stage single-factor experiment, among the five variables studied, only pH, NH4NO3 concentration, and Fe concentration affected siderophore synthesis. In the second stage, an optimization of pH, NH4NO3 concentration, and Fe concentration for improved growth and enhanced siderophore production was carried out using a Box–Behnken design with response surface methodology. By using LC-MS, two derivatives of pseudomonine, salicylic acid, and kynurenic acid were detected as siderophores in the purified XAD-2 methanol extract of the P. monteilii strain MN759447. In addition to siderophore production, the P. monteilii strain MN759447 also exhibited a broad range of antagonistic activity against Aspergillus calidoustus (65%), Fusarium oxysporum (41.66%), Talaromyces pinophilus (65%), and Talaromyces verruculosus (65.1%) that are linked to sissoo mortality. To our knowledge, this is the first report on siderophore-producing bacteria isolated, identified, and characterized from the D. sissoo Roxb. forest habitat. This strain can also be developed as a commercial product.

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Unboxing PGPR-mediated management of abiotic stress and environmental cleanup: what lies inside?

Kumar,

Ali,

Sharma

et al. 2024

Environ Sci Pollut Res

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Contrasting effects of siderophores pyoverdine and desferrioxamine B on the mobility of iron, aluminum, and copper in Cu-contaminated soils

Cited by 7 publications

References 48 publications

Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts

Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts

Optimization and identification of siderophores produced by Pseudomonas monteilii strain MN759447 and its antagonism toward fungi associated with mortality in Dalbergia sissoo plantation forests

Unboxing PGPR-mediated management of abiotic stress and environmental cleanup: what lies inside?

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