“…The Psa3 V-13 complete effector knockout strain was generated using the pK18mobsacB-based vectors used previously to generate single knockout strains (Hemara et al, 2022; Jayaraman et al, 2023). The single effectors or effector blocks were sequentially knocked out to generate the 33 effector knockout strain (Psa3 V-13 Δ 33E ) in the order: hopZ5a/hopH1a ( using the hopZ5a/hopH1a double knockout vector ), hopBP1a ( previously hopZ3), hopQ1a, hopAS1b, avrPto1b ( previously avrPto5), avrRpm1a, fEEL (avrD1/avrB2b/hopF4a/hopAW1a/hopF1e/hopAF1b/hopD2a/hopF1a), hopF1c ( previously hopF2), hopD1a ( using the hopQ1a/hopD1a double knockout vector ), CEL (hopN1a/hopM1f/avrE1d), hopR1b, hopAZ1a, hopS2b, hopY1b, hopAM1a-1, hopAM1a-2, hopBN1a, hopW1c (previously hopAE1), hopAU1a, hopI1c , and hopAH1 block (hopAG1f/hopAH1c/hopAI1b ).…”