Contrasting dynamics of radial O2-loss barrier induction and aerenchyma formation in rice roots of two lengths

Shiono, Katsuhiro; Ogawa, Satoshi; Yamazaki, So; Isoda, Hiroko; Fujimura, Tatsuhito; Nakazono, Mikio; Colmer, Timothy D.

doi:10.1093/aob/mcq221

Cited by 127 publications

(132 citation statements)

References 34 publications

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“…In transverse sections, GOS2::OsERF71 and RCc3::OsERF71 roots had larger aerenchyma (Fig. 4, B and D-F), which are air spaces between epidermal and endodermal cell layers that provide an internal pathway for oxygen movement (Shiono et al, 2011). They also developed approximately four cell layers between the mature metaxylem cells (Fig.…”

Section: Root Overexpression Of Oserf71 Alters Radial Root Growthmentioning

confidence: 99%

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

et al. 2016

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Plant responses to drought stress require the regulation of transcriptional networks via drought-responsive transcription factors, which mediate a range of morphological and physiological changes. AP2/ERF transcription factors are known to act as key regulators of drought resistance transcriptional networks; however, little is known about the associated molecular mechanisms that give rise to specific morphological and physiological adaptations. In this study, we functionally characterized the rice (Oryza sativa) drought-responsive AP2/ERF transcription factor OsERF71, which is expressed predominantly in the root meristem, pericycle, and endodermis. Overexpression of OsERF71, either throughout the entire plant or specifically in roots, resulted in a drought resistance phenotype at the vegetative growth stage, indicating that overexpression in roots was sufficient to confer drought resistance. The root-specific overexpression was more effective in conferring drought resistance at the reproductive stage, such that grain yield was increased by 23% to 42% over wild-type plants or whole-body overexpressing transgenic lines under drought conditions. OsERF71 overexpression in roots elevated the expression levels of genes related to cell wall loosening and lignin biosynthetic genes, which correlated with changes in root structure, the formation of enlarged aerenchyma, and high lignification levels. Furthermore, OsERF71 was found to directly bind to the promoter of OsCINNAMOYL-COENZYME A REDUCTASE1, a key gene in lignin biosynthesis. These results indicate that the OsERF71-mediated drought resistance pathway recruits factors involved in cell wall modification to enable root morphological adaptations, thereby providing a mechanism for enhancing drought resistance.

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Section: Root Overexpression Of Oserf71 Alters Radial Root Growthmentioning

confidence: 99%

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

et al. 2016

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“…Barrier induction is weak in shorter roots but strong in longer roots, indicating that root tissue age/ development stage influences the ROL barrier Shiono et al, 2011). Barrier induction commences within the first day upon exposure of a previously aerated root system to stagnant conditions .…”

Section: Root Rol Barrier Formation Root Barriers Can Restrict Rol Frmentioning

confidence: 99%

Regulation of Root Traits for Internal Aeration and Tolerance to Soil Waterlogging-Flooding Stress

et al. 2017

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“…To adapt to waterlogging in soil, rice (Oryza sativa) develops lysigenous aerenchyma in shoots (Matsukura et al, 2000;Colmer and Pedersen, 2008;Steffens et al, 2011) and roots (Jackson et al, 1985b;Justin and Armstrong, 1991;Kawai et al, 1998), which is formed by programmed cell death and subsequent lysis of some cortical cells (Jackson and Armstrong, 1999;Evans, 2004;Yamauchi et al, 2013). In rice roots, lysigenous aerenchyma is constitutively formed under aerobic conditions (Jackson et al, 1985b), and its formation is further induced under oxygen-deficient conditions Shiono et al, 2011). The former and latter are designated constitutive and inducible lysigenous aerenchyma formation, respectively (Colmer and Voesenek, 2009).…”

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confidence: 99%

Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots

et al. 2015

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In rice (Oryza sativa) roots, lysigenous aerenchyma, which is created by programmed cell death and lysis of cortical cells, is constitutively formed under aerobic conditions, and its formation is further induced under oxygen-deficient conditions. Ethylene is involved in the induction of aerenchyma formation. reduced culm number1 (rcn1) is a rice mutant in which the gene encoding the ATP-binding cassette transporter RCN1/OsABCG5 is defective. Here, we report that the induction of aerenchyma formation was reduced in roots of rcn1 grown in stagnant deoxygenated nutrient solution (i.e. under stagnant conditions, which mimic oxygen-deficient conditions in waterlogged soils). 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a key enzyme in ethylene biosynthesis. Stagnant conditions hardly induced the expression of ACS1 in rcn1 roots, resulting in low ethylene production in the roots. Accumulation of saturated very-long-chain fatty acids (VLCFAs) of 24, 26, and 28 carbons was reduced in rcn1 roots. Exogenously supplied VLCFA (26 carbons) increased the expression level of ACS1 and induced aerenchyma formation in rcn1 roots. Moreover, in rice lines in which the gene encoding a fatty acid elongase, CUT1-LIKE (CUT1L; a homolog of the gene encoding Arabidopsis CUT1, which is required for cuticular wax production), was silenced, both ACS1 expression and aerenchyma formation were reduced. Interestingly, the expression of ACS1, CUT1L, and RCN1/OsABCG5 was induced predominantly in the outer part of roots under stagnant conditions. These results suggest that, in rice under oxygen-deficient conditions, VLCFAs increase ethylene production by promoting 1-aminocyclopropane-1-carboxylic acid biosynthesis in the outer part of roots, which, in turn, induces aerenchyma formation in the root cortex.Aerenchyma formation is a morphological adaptation of plants to complete submergence and waterlogging of the soil, and facilitates internal gas diffusion (Armstrong,

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Contrasting dynamics of radial O2-loss barrier induction and aerenchyma formation in rice roots of two lengths

Cited by 127 publications

References 34 publications

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

Overexpression of the OsERF71 Transcription Factor Alters Rice Root Structure and Drought Resistance

Regulation of Root Traits for Internal Aeration and Tolerance to Soil Waterlogging-Flooding Stress

Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots

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