Contrasting detoxification mechanisms of Chlamydomonas reinhardtii under Cd and Pb stress

Li, Chonghua; Zheng, Chongke; Fu, Hongxuan; Zhai, Suhua; Hu, Fan; Naveed, Sadiq; Zhang, Chunhua; Ge, Ying

doi:10.1016/j.chemosphere.2021.129771

Cited by 66 publications

(33 citation statements)

References 62 publications

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“…Though, from the 13th day of culture, it was maintained at a stationary level. This finding is consistent with previous research reporting the inhibitory effect of Pb(II) ions against Chlamydomonas reinhardtii(Li et al 2021).…”

supporting

confidence: 93%

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Danouche

Aasfar

et al. 2021

Preprint

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This study highlights the mechanisms involved in Pb(II)-phycoremediation using the Pb(II) tolerant strain of Scenedesmus obliquus. Firstly, monitoring of cell growth kinetics in control and Pb(II)-doped medium revealed a significant growth inhibition, while the analyses through flow cytometry and Zetasizer revealed no difference in cell viability and size. Residual weights of control and Pb(II)-loaded cells assessed by thermogravimetric analysis, were 31.34% and 57.8%, respectively, indicating the uptake of Pb(II) into S. obliquus cells. Next, the use of chemical extraction to distinguish between the intracellular and extracellular uptake indicated the involvement of both biosorption (85.5%) and bioaccumulation (14.5%) mechanisms. Biosorption interaction of the cell wall and Pb(II) was confirmed by SEM, EDX, FTIR, zeta potential, zero-charge pH, and contact angle analyses. Besides, the biochemical characterization of control and Pb(II)-loaded cells revealed that the bioaccumulation of Pb(II) induces significant increases in the carotenoids and lipids content and decreases in the proportions of chlorophyll, carbohydrates, and proteins. Finally, the metabolomic analysis indicated an increase in the relative abundance of fatty acid methyl esters, alkanes, aromatic compounds, and sterols. However, the alkenes and monounsaturated fatty acids decreased. Such metabolic adjustment may represent an adaptive strategy, that preventing high Pb(II)-bioaccumulation in cellular compartments.

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supporting

confidence: 93%

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Danouche

Aasfar

et al. 2021

Preprint

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“…Based on the complexation coefficients of various Pb complexes (Supplementary Table S1), biological samples “stained” by Pb alone may also demonstrate well-resolved structure under TEM (Bringezu et al, 1999). Previous studies had shown well-defined intra- and extracellular morphologies after Pb staining (Tian et al, 2019; Jiang et al, 2020 b ; Li et al, 2021). Therefore, the application of Pb could be a potential way to stain ultrathin sections of microbial cells.…”

Section: Introductionmentioning

confidence: 86%

“…C. reinhardtii was purchased from the Chlamydomonas Resource Center, Department of Plant and Biological Biology, University of Minnesota, USA. It was activated in Tris-Acetate-Phosphate (TAP) medium (Li et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

Transmission Electron Microscopy Analysis on Microbial Ultrathin Sections Prepared by the Ultra-Low Lead Staining Technique

et al. 2021

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“…(2019) , LB-EPS of Chlamydomonas reinhardtii had increased significantly after Cd-exposure. Recently, Li et al. (2021) indicted also an increase in EPS production by C. reinhardtii under Pb(II) and Cd(II) stresses.…”

Section: Mechanisms Of Hms Phycoremediation Using Living Microalgaementioning

confidence: 98%

“…also in S. bijugatus Cu(II)-treated ( Nagalakshmi and Prasad, 2001 ), and Pb (II)-treated Stichococcus bacillaris ( Pawlik-skowron, 2002 ). Furthermore, it has been reported that Cd(II) was the most effective stimulator of PCs synthase of Chlamydomonas species, while other metals have shown less effectiveness in various degrees ( Abboud and Wilkinson, 2013 ; Kobayashi et al., 2006 ; Li et al., 2021 ; Suárez et al., 2010 ). Nevertheless, PC synthesis was most strongly induced by Zn in Dunaliella species ( Hirata et al., 2001 ; Tsuji et al., 2003 ).…”

Section: Mechanisms Of Hms Phycoremediation Using Living Microalgaementioning

confidence: 99%

Phycoremediation mechanisms of heavy metals using living green microalgae: physicochemical and molecular approaches for enhancing selectivity and removal capacity

Danouche

Ghachtouli

Arroussi

2021

Heliyon

118

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Heavy metal (HM) contamination of water bodies is a serious global environmental problem. Because they are not biodegradable, they can accumulate in food chains, causing various signs of toxicity to exposed organisms, including humans. Due to its effectiveness, low cost, and ecological aspect, phycoremediation, or the use of microalgae's ecological functions in the treatment of HMs contaminated wastewater, is one of the most recommended processes. This study aims to examine in depth the mechanisms involved in the phycoremediation of HMs by microalgae, it also provides an overview of the prospects for improving the productivity, selectivity, and cost-effectiveness of this bioprocess through physicochemical and genetic engineering applications. Firstly, this review proposes a detailed examination of the biosorption interactions between cell wall functional groups and HMs, and their complexation with extracellular polymeric substances released by microalgae in the extracellular environment under stress conditions. Subsequently, the metal transporters involved in the intracellular bioaccumulation of HMs as well as the main intracellular mechanisms including compartmentalization in cell organelles, enzymatic biotransformation, or photoreduction of HMs were also extensively reviewed. In the last section, future perspectives of physicochemical and genetic approaches that could be used to improve the phytoremediation process in terms of removal efficiency, selectivity for a targeted metal, or reduction of treatment time and cost are discussed, which paves the way for large-scale application of phytoremediation processes.

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Contrasting detoxification mechanisms of Chlamydomonas reinhardtii under Cd and Pb stress

Cited by 66 publications

References 62 publications

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Mechanisms of Lead (II) Phycoremediation Using Scenedesmus Obliquus: Cell Physicochemical Properties, Biochemical Composition and Metabolomic Profiling

Transmission Electron Microscopy Analysis on Microbial Ultrathin Sections Prepared by the Ultra-Low Lead Staining Technique

Phycoremediation mechanisms of heavy metals using living green microalgae: physicochemical and molecular approaches for enhancing selectivity and removal capacity

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