Contrasting bone effects of temporary versus permanent IGFBP administration in rodents

Hoeflich, Andreas

doi:10.1016/j.ghir.2008.01.003

Cited by 7 publications

(5 citation statements)

References 49 publications

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“…Increase of IGFBP2 has been shown to delay development in fetuses and reduce BW and skeleton growth (Tapanainen et al, 1994;Hoeflich, 2008), and also impair IGF-1 promotion of mammary gland development (Hadsell et al, 1996;Wolf et al, 1997). Coincidentally, in our study, high immunolabeling of IGFBP2 in untreated heifers was related with lower development of the parenchyma (Perri et al, 2013) and also a lower rate of cell division.…”

Section: Discussionsupporting

confidence: 70%

Cellular proliferation rate and insulin-like growth factor binding protein (IGFBP)-2 and IGFBP-3 and estradiol receptor alpha expression in the mammary gland of dairy heifers naturally infected with gastrointestinal nematodes during development

Perri

Dallard

Baravalle

et al. 2014

Journal of Dairy Science

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Mammary ductal morphogenesis during prepuberty occurs mainly in response to insulin-like growth factor-1 (IGF-1) and estradiol stimulation. Dairy heifers infected with gastrointestinal nematodes have reduced IGF-1 levels, accompanied by reduced growth rate, delayed puberty onset, and lower parenchyma-stroma relationship in their mammary glands. Immunohistochemical studies were undertaken to determine variations in cell division rate, IGF-1 system components, and estradiol receptors (ESR) during peripubertal development in the mammary glands of antiparasitic-treated and untreated Holstein heifers naturally infected with gastrointestinal nematodes. Mammary biopsies were taken at 20, 30, 40, and 70 wk of age. Proliferating cell nuclear antigen immunolabeling, evident in nuclei, tended to be higher in the parenchyma of the glands from treated heifers than in those from untreated. Insulin-like growth factor binding proteins (IGFBP) type 2 and type 3 immunolabeling was cytoplasmic and was evident in stroma and parenchyma. The IGFBP2-labeled area was lower in treated than in untreated heifers. In the treated group, a maximal expression of this protein was seen at 40 wk of age, whereas in the untreated group the labeling remained constant. No differences were observed for IGFBP3 between treatment groups or during development. Immunolabeling for α ESR (ESR1) was evident in parenchymal nuclei and was higher in treated than in untreated heifers. In the treated group, ESR1 peaked at 30 wk of age and then decreased. These results demonstrate that the parasite burden in young heifers negatively influence mammary gland development, affecting cell division rate and parameters related to estradiol and IGF-1 signaling in the gland.

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Section: Discussionsupporting

confidence: 70%

Cellular proliferation rate and insulin-like growth factor binding protein (IGFBP)-2 and IGFBP-3 and estradiol receptor alpha expression in the mammary gland of dairy heifers naturally infected with gastrointestinal nematodes during development

Perri

Dallard

Baravalle

et al. 2014

Journal of Dairy Science

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“…While the role of IGFBPs in other tissues such as bone is relatively well understood [18][19][20][21], there is a dearth of information regarding the role of IGFBPs in skeletal muscle, particularly in the pathophysiology of muscular dystrophy. Of the IGFBP family, only IGFBP-5 has been directly investigated in muscular dystrophy, with increased expression in DMD fibroblasts correlating with impaired DMD myoblast growth in vitro [22].…”

Section: Introductionmentioning

confidence: 99%

“…Increased serum IGFBP-2 concentrations have been correlated with low body weight in male mice [18]. Furthermore, mice with systemic over-expression of IGFBP-2 are significantly smaller than their non-transgenic littermates and have smaller myofibers [27,29,33].…”

Section: Introductionmentioning

confidence: 99%

Skeletal muscle-specific overexpression of IGFBP-2 promotes a slower muscle phenotype in healthy but not dystrophic mdx mice and does not affect the dystrophic pathology

Swiderski

Martins

Chee

et al. 2016

Growth Hormone & IGF Research

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“…The longer-term inhibitory effects of soluble IGFBP2 (e.g., on cell proliferation [13]) can be neglected in the new bioassay. IGFBP effects as a function of the duration of IGFBP exposure were also discussed in vivo [20]. Notably, insulin-stimulated AKT phosphorylation at supraphysiological concentrations and preincubation with IGFBP2 did not affect insulin sensitivity in our system.…”

Section: Short-term Effects Of Membrane-bound Igfbp2 Vs Longer-term mentioning

confidence: 72%

Development of a Sensitive Bioassay for the Analysis of IGF-Related Activation of AKT/mTOR Signaling in Biological Matrices

Walz

Höflich

Walz

et al. 2021

Cells

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The bioactivity of the IGF system is not a function of isolated hormone concentrations in a given biological matrix. Instead, the biological activities of IGFs are regulated by IGFBPs, IGFBP proteases, and inhibitors of IGFBP proteases. Therefore, assays based on IGF-related bioactivity may describe functions of the complete IGF system in a given biological matrix. Of particular interest are the IGF system effects on the AKT/mTOR pathway, as a dominant system for controlling growth, metabolism, and aging. In order to improve the sensitivity of IGF-dependent bioactivity, we made use of the known short-term and enhancing effects of IGFBP2 on the intracellular PI3K pathway. As a specific readout of this pathway, and further as a marker of the mTOR pathway, we assessed the phosphorylation of AKT-Ser473. Preincubation using IGFBP2 enhanced IGF1-dependent AKT-Ser473 phosphorylation in our experimental system. The assay’s specificity was demonstrated by inhibition of IGF1 receptors outside or inside the cell, using antiserum or small molecule inhibitors, which reduced AKT phosphorylation in response to exogenous IGF1 (p < 0.05). The maximal response of AKT phosphorylation was recorded 15 to 60 min after the addition of IGF1 to cell monolayers (p < 0.001). In our cellular system, insulin induced AKT phosphorylation only at supra-physiological concentrations (µM). Using this novel assay, we identified the differential biological activity of the IGF system in AKT-Ser473 phosphorylation in serum (mouse, naked mole rat, and human), in cerebrospinal fluid (human), and in colostrum or mature milk samples (dairy cow). We have developed a sensitive and robust bioassay to assess the IGF-related activation of the AKT/mTOR pathway. The assay works efficiently and does not require expensive cell culture systems. By using capillary immuno-electrophoresis, the readout of IGF-related bioactivity is substantially accelerated, requiring a minimum of hands-on time. Importantly, the assay system is useful for studying IGF-related activity in the AKT/mTOR pathway in a broad range of biological matrices.

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Contrasting bone effects of temporary versus permanent IGFBP administration in rodents

Cited by 7 publications

References 49 publications

Cellular proliferation rate and insulin-like growth factor binding protein (IGFBP)-2 and IGFBP-3 and estradiol receptor alpha expression in the mammary gland of dairy heifers naturally infected with gastrointestinal nematodes during development

Cellular proliferation rate and insulin-like growth factor binding protein (IGFBP)-2 and IGFBP-3 and estradiol receptor alpha expression in the mammary gland of dairy heifers naturally infected with gastrointestinal nematodes during development

Skeletal muscle-specific overexpression of IGFBP-2 promotes a slower muscle phenotype in healthy but not dystrophic mdx mice and does not affect the dystrophic pathology

Development of a Sensitive Bioassay for the Analysis of IGF-Related Activation of AKT/mTOR Signaling in Biological Matrices

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