Contrast Stain for the Rapid Identification of Trichomonas Vaginalis

Miller, Julia R.

doi:10.1093/ajcp/7.ts1.2.40

Cited by 9 publications

(2 citation statements)

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“…Other stains such as safranin, methylene blue, and malachite green, which do not stain trophozoites, can act as counterstains [ 76 , 77 , 78 ]. Fluorescein can also be used to observe wet mount slides under an ultraviolet light microscope [ 79 ].…”

Section: Classical Diagnostic Procedures: Microscopymentioning

confidence: 99%

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Ibáñez-Escribano,

Nogal-Ruiz

2024

Pathogens

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More than one million curable sexually transmitted infections occur every day. Trichomonas vaginalis is one of the main infections responsible for these epidemiological data; however, the diagnosis of this protozoan is still mainly based on microscopic and culture identification. The commercialization of immunological tests and the development of molecular techniques have improved the sensitivity of classical methods. Nevertheless, the fact that trichomoniasis is a neglected parasitic infection hinders the development of novel techniques and their implementation in routine diagnosis. This review article shows the different methods developed to identify T. vaginalis in population and the difficulties in diagnosing male and asymptomatic patients. The importance of including this parasite in routine gynecological screening, especially in pregnant women, and the importance of considering T. vaginalis as an indicator of high-risk sexual behavior are also discussed.

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Section: Classical Diagnostic Procedures: Microscopymentioning

confidence: 99%

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Ibáñez-Escribano,

Nogal-Ruiz

2024

Pathogens

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“…Certain features, such as spindle shaped nucleus, the contours of the organism, and the cytoplasmic inclusions, allow easy identification of the organism. Although trichomonads are not stained by safranin, malachite green, methylene blue, and brilliant cresyl blue, other cellular material takes up the dye and the organisms stand out against this colored background [70][71][72]. However, these staining methods has not been shown convincingly to improve the detection rate of trichomonads in secretions and is not recommended for the routine clinical laboratory.…”

Section: Staining Techniquesmentioning

confidence: 99%

The Past, Present and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Ibáñez-Escribano,

Nogal-Ruiz

2023

Preprint

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More than one million curable sexually transmitted infections occur every day. Trichomonas vaginalis is one of the main responsible for these epidemiological data, however, the diagnosis of this protozoan is still mainly based on microscopic and culture identification. The commerciali-zation of immunological tests and the development of molecular techniques have improved the sensitivity of classical methods. Nevertheless, the fact that trichomoniasis is a neglected parasitic infection, hinders the development of novel techniques and their implementation in routine di-agnosis. This review article shows the different methods developed to identify T. vaginalis in population and the difficulties to diagnose male and asymptomatic patients. The importance of including this parasite in routine gynecological screening, especially in pregnant women, and the importance of considering T. vaginalis as an indicator of high-risk sexual behavior is also discussed.

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The effect of venous occlusion on determination of protein-bound iodine

Lewitus

Steinitz

1963

Clinica Chimica Acta

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Contrast Stain for the Rapid Identification of Trichomonas Vaginalis

Cited by 9 publications

References 0 publications

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

The Past, Present and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

The effect of venous occlusion on determination of protein-bound iodine

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