Contrast Harmonic Imaging Characterization of Canine Splenic Lesions

Abstract

Background: Although B-mode ultrasound is very sensitive for the detection of splenic lesions, its specificity is low. Contrast harmonic imaging is used successfully to differentiate benign from malignant liver lesions in humans and dogs.Hypothesis: Contrast harmonic imaging could be useful to differentiate benign and malignant splenic lesions in dogs. Animals: Sixty dogs (clinical patients) with splenic abnormalities detected during abdominal ultrasonography. Methods: A prospective study was performed with a Philips ATL 5000 unit for contrast pulse inversion harmonic imaging (mechanical index: 0.08, contrast medium: SonoVue). Perfusion was assessed subjectively and quantitatively.Results: Cytology or histology identified 27 benign (hyperplasia, extramedullary hematopoiesis, hematoma) and 29 malignant (hemangiosarcoma, malignant lymphoma, malignant histiocytosis, mesenchymal tumors without classification, mast cell tumors, and others) lesions and 4 normal spleens. Except for 1 benign nodule, extensive to moderate hypoechogenicity was only seen in malignant lesions during wash-in, at peak enhancement, and during wash-out (P 5 .0001, odds ratios: 37.9 [95% CI 4.5-316.5], 66.4 [95% CI 8.0-551.1], and 36.9 [95% CI 4.4-308.4]). Although all but 1 benign lesion enhanced well and were mildly hypo-, iso-, or hyperechoic in comparison with the normal spleen during all blood pool phases, marked enhancement occurred both in benign as well as in malignant splenic lesions. Quantitative perfusion values did not differ significantly between benign and malignant lesions.Conclusions and Clinical Importance: Moderate to extensive hypoechogenicity clearly identifies canine splenic malignant lesions. In nodules with marked enhancement, contrast harmonic ultrasound is of limited value and histology is needed.

Safety of contrast-enhanced ultrasonography in dogs and cats: 488 cases (2002–2011)

“…Results of multiple studies [14][15][16][17][18] in which CEUS was used in dogs and cats suggest that CEUS is a clinically valuable diagnostic procedure, and no adverse events associated with injection of various contrast agents were reported. Results of multiple studies [14][15][16][17][18] in which CEUS was used in dogs and cats suggest that CEUS is a clinically valuable diagnostic procedure, and no adverse events associated with injection of various contrast agents were reported.…”

Quantitative perfusion analysis of the pancreas and duodenum in healthy dogs by use of contrast-enhanced ultrasonography

“…2 Pancreatic changes can manifest sonographically in various appearances depending on the severity and chronicity of the disease. Published reports describe the use of contrast-enhanced ultrasonography to quantify healthy vascular perfusion in the liver, 22 kidney, 23 spleen, 24 and prostate 25,26 ; to characterize perfusion patterns of lesions within the liver, 27,28 spleen, 28,29 and kidney 30 ; for the diagnosis of portosystemic shunts 31 ; and to characterize vascular patterns within lymphomatous lymph nodes. Pancreatitis can have an unpredictable course, and prognosis is difficult to ascertain.…”

Spleen

“…[84][85][86] Ohlerth and colleagues 85 found moderate to extensive hypoechoic lesions clearly identified malignant splenic lesions in dogs, whereas Fifteen-year-old neutered male cat with pancytopenia due to bone marrow arrest. [84][85][86] Ohlerth and colleagues 85 found moderate to extensive hypoechoic lesions clearly identified malignant splenic lesions in dogs, whereas Fifteen-year-old neutered male cat with pancytopenia due to bone marrow arrest.…”