Contrast enhancement of nanomaterials using phase plate STEM

Minoda, Hiroki; Tamai, Takayuki; Ohmori, Yuya; Iijima, Hirofumi

doi:10.1016/j.ultramic.2017.07.006

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…This achieves super-resolution three-dimensional reconstruction without electron staining in FIB/ SEM technology. Moreover, combining super-resolution CL imaging with recently developed phase contrast scanning transmission electron imaging using an environmental cell 20,21 opens up the possibility of simultaneous observation of light and electron images with extreme spatial resolution without chemical staining.…”

Section: Resultsmentioning

confidence: 99%

Cathodoluminescence of green fluorescent protein exhibits the redshifted spectrum and the robustness

Akiba

Tamehiro

Matsui

et al. 2020

Sci Rep

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Green fluorescent protein (GFP) and its variants are an essential tool for visualizing functional units in biomaterials. This is achieved by the fascinating optical properties of them. Here, we report novel optical properties of enhanced GFP (EGFP), which is one of widely used engineered variants of the wild-type GFP. We study the electron-beam-induced luminescence, which is known as cathodoluminescence (CL), using the hybrid light and transmission electron microscope. Surprisingly, even from the same specimen, we observe a completely different dependences of the fluorescence and CL on the electron beam irradiation. Since light emission is normally independent of whether an electron is excited to the upper level by light or by electron beam, this difference is quite peculiar. We conclude that the electron beam irradiation causes the local generation of a new redshifted form of EGFP and CL is preferentially emitted from it. In addition, we also find that the redshifted form is rather robust to electron bombardment. These remarkable properties can be utilized for three-dimensional reconstruction without electron staining in focused ion beam/scanning electron microscopy technology and provide significant potential for simultaneously observing the functional information specified by super-resolution CL imaging and the structural information at the molecular level obtained by electron microscope.

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Section: Resultsmentioning

confidence: 99%

Cathodoluminescence of green fluorescent protein exhibits the redshifted spectrum and the robustness

Akiba

Tamehiro

Matsui

et al. 2020

Sci Rep

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“…Misaki Tsubouchi 1 and Hiroki Minoda 2 1 Tokyo University of Agriculture and Technology, United States, 2 Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan…”

Section: Increased Efficiency Of Phase Plate Stem Using 2d Detectormentioning

confidence: 99%

“…The PP can apply a phase shift according to its pattern to the incident electron wave and can modify the phase contrast transfer function (PCTF). Since only the electrons within the small collection angle have beta to be used to realize the same optical condition as that of phase plate TEM [1,2], we can use only a small fraction of the electrons arrive onto a detector plane. Therefore, low electron efficiency was a critical problem so far.…”

Section: Increased Efficiency Of Phase Plate Stem Using 2d Detectormentioning

confidence: 99%

Increased efficiency of phase plate STEM using 2D detector

Tsubouchi¹,

Minoda

2021

Microsc Microanal

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“…That's because the sine type PCTF of the conventional STEM is almost zero at low spatial frequency and image contrast of such samples can't be obtained. Our previous STEM study shows the validity of the ZPP to visualize nm scale materials [1,2].…”

mentioning

confidence: 97%