Contrast enhancement by oblique illumination microscopy with an LED array

Sugimoto, Ryo; Maruyama, Ryoji; Tamada, Yosuke; Arimoto, Hidenobu; Watanabe, Wataru

doi:10.1016/j.ijleo.2019.02.068

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Based on this research, we extended our study to develop a new (to the best of our knowledge) method [94] of photonic hook-based near-field oblique illumination microscopy with super contrast subwavelength imaging. It was experimentally demonstrated [31,95] for the first time that the Janus particle [96][97][98] provides oblique illumination [99][100][101] of imaging objects by localized structured curved beams, which leads to a considerable improvement in the near-field imaging contrast without the use of immersion medium (Figure 4). One can see that the oblique illumination (illumination by PH) results in the diffracted order of +1 on the particle, which finally allows increasing the image contrast.…”

Section: Photonic Hook Based High-contrast Subwavelength Imagingmentioning

confidence: 99%

Mesotronics: Some New, Unusual Optical Effects

Minin

2022

Photonics

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The recently emerged field of Mesotronics provides novel opportunities for subwavelength magnetic and field localization and giant enhancement by mesoscale dielectric particles and structures from low-index to high-index materials, supported by novel optical phenomena. In this case, two regions: non-resonant and resonant, can be distinguished. In this short review, which is a direct continuation of our recently published study, we continue to present the authors’ point of view on some new optical effects in dielectric mesotronics. Among them are anomalous apodization effect in phase mesoscale gratings, new effects on high order Fano-resonances and extreme effects in field localization, mesoscale particle-based super-resolution and high-speed communications, photonic hook-based high-contrast subwavelength imaging, and reverse optical energy flow in a perforated resonant spherical particle.

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Section: Photonic Hook Based High-contrast Subwavelength Imagingmentioning

confidence: 99%

Mesotronics: Some New, Unusual Optical Effects

Minin

2022

Photonics

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“…The microscope consists of a part of an upright microscope (BX43; Olympus Corp.). A condenser lens was removed, and an LED array was placed [20]. An RGB full-color dot matrix LED array (8 × 8 dots, NeoPixel NeoMatrix 64 RGBW LED; Adafruit Industries) was placed 35 mm below the sample.…”

Section: Optical Setup For An Led Array Microscopementioning

confidence: 99%

Acquisition of Multi-Modal Images of Structural Modifications in Glass with Programmable LED-Array-Based Illumination

2019

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Ultrashort laser pulses can induce structural modifications in bulk glass, leading to refractive index change and scattering damage. As bright-field, dark-field, and phase imaging each provide complementary information about laser-induced structures, it is often desired to use multiple observations simultaneously. As described herein, we present the acquisition of bright-field, dark-field, and differential phase-contrast images of structural modifications induced in glass by femtosecond laser pulses with an LED array microscope. The contrast of refractive index change can be enhanced by differential phase-contrast images. We also report on the simultaneous acquisition of bright-field and dark-field images of structural modifications in a glass with LED-array-based Rheinberg illumination. A single-shot color image is separated to obtain bright field and dark field images simultaneously. We provide an experimental demonstration on multi-modal imaging of structural modifications in a glass with an LED array microscope using temporally-coded illumination and color-coded illumination.

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“…Due to its simple structure, OIM can be easily modified by a BF microscope to improve image contrast. Therefore, this microscopy has features of low cost and high contrast, that can be used in different samples such as diatoms [20] and live buccal epithelial cells [21]. Traditional oblique illumination imaging methods improve the contrast of transparent objects by offsetting condensers or blocking parts of imaging lighting [22].…”

Section: Introductionmentioning

confidence: 99%

An apparatus for qualitative assessment of the shading ratio of oblique illumination and real‐time high‐contrast imaging

Shao

Wang

et al. 2022

Journal of Biophotonics

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Oblique illumination imaging can significantly improve the contrast of transparent thin samples. However, in traditional oblique illumination methods, either the condenser is offset or a block is added to the condenser, which makes it complicated and challenged to build a stable oblique illumination imaging. Herein, we present a method to measure the optimal shading ratio of oblique illumination in an inverted microscope, and develop an apparatus for stable high‐speed high‐contrast imaging with uniform brightness. At optimal shading ratio, the oblique illumination imaging has better imaging quality than differential interference contrast, which characteristic is independent on sample. In oblique illumination with low magnification objective, the images have uneven brightness. According to target brightness, we have developed a brightness unevenness correction algorithm to form uniform background brightness for oblique illumination. Integrating the algorithm with imaging acquisition, corrected oblique illumination microscopy is appropriate to observe living cells with high contrast.

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Contrast enhancement by oblique illumination microscopy with an LED array

Cited by 5 publications

References 15 publications

Mesotronics: Some New, Unusual Optical Effects

Mesotronics: Some New, Unusual Optical Effects

Acquisition of Multi-Modal Images of Structural Modifications in Glass with Programmable LED-Array-Based Illumination

An apparatus for qualitative assessment of the shading ratio of oblique illumination and real‐time high‐contrast imaging

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