Contractile properties and creatine kinase activity of myofilaments following ischemia and reperfusion of the rat heart

Ventura‐Clapier, Renée; Veksler, Vladimir; Elizarova, Galina V.; Mekhfi, Hassane; Levitskaya, E. L.; Saks, Valdur

doi:10.1016/0885-4505(87)90094-6

Cited by 13 publications

(3 citation statements)

References 20 publications

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“…Although in two studies, myofibril-bound MM-CK was found to be preserved despite a loss in total CK activity (509,1062), its activity was reported to be depressed by others (304). Upon prolonged ischemia, redistribution of M-CK was observed in immunoelectron micrographs of the dog heart, with a progressive loss of M-CK from the myofibrillar A band (740).…”

Section: The Response Of the Ck/pcr/cr System To Low-oxygen Stress Anmentioning

confidence: 98%

“…Again, the factors determining irreversible functional and biochemical deterioration of the heart are largely unknown despite a variety of hypotheses raised. Notably, even after prolonged ischemia and reperfusion, skinned ventricular strips from rat hearts displayed normal resting tension, maximal tension, Ca 2ϩ sensitivity, and response to quick length changes in the presence of both PCr and MgATP (1062), thus excluding a functional defect at the level of the myofilaments. What, hence, are the disturbances of the CK/PCr/Cr system induced by low-oxygen stress and subsequent reperfusion, and how do they relate to the contractile dysfunction observed?…”

Section: Introductionmentioning

confidence: 96%

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Creatine and Creatinine Metabolism

Wyss

Kaddurah‐Daouk

2000

Physiological Reviews

2,352

2,103

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The goal of this review is to present a comprehensive survey of the many intriguing facets of creatine (Cr) and creatinine metabolism, encompassing the pathways and regulation of Cr biosynthesis and degradation, species and tissue distribution of the enzymes and metabolites involved, and of the inherent implications for physiology and human pathology. Very recently, a series of new discoveries have been made that are bound to have distinguished implications for bioenergetics, physiology, human pathology, and clinical diagnosis and that suggest that deregulation of the creatine kinase (CK) system is associated with a variety of diseases. Disturbances of the CK system have been observed in muscle, brain, cardiac, and renal diseases as well as in cancer. On the other hand, Cr and Cr analogs such as cyclocreatine were found to have antitumor, antiviral, and antidiabetic effects and to protect tissues from hypoxic, ischemic, neurodegenerative, or muscle damage. Oral Cr ingestion is used in sports as an ergogenic aid, and some data suggest that Cr and creatinine may be precursors of food mutagens and uremic toxins. These findings are discussed in depth, the interrelationships are outlined, and all is put into a broader context to provide a more detailed understanding of the biological functions of Cr and of the CK system.

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Section: The Response Of the Ck/pcr/cr System To Low-oxygen Stress Anmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 96%

Creatine and Creatinine Metabolism

Wyss

Kaddurah‐Daouk

2000

Physiological Reviews

2,352

2,103

View full text Add to dashboard Cite

show abstract

“…The CK-MB activity is obtained by multiplying the CK-B activity by two. By measuring the variation of the absorbance due to transformation of β-NADP+ into β-NADPH in a time interval at 340 nm, it is possible to calculate the residual activity in the examined sample [ 29 , 32 ]. Methodology: IFCC Method/Immunoinhibition.…”

Section: Methodsmentioning

confidence: 99%

The Coadministration of Levosimendan and Exenatide Offers a Significant Cardioprotective Effect to Isolated Rat Hearts against Ischemia/Reperfusion Injury

Leivaditis

Koletsis

Tsopanoglou

et al. 2022

JCDD

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(1) Background: The present study aims to investigate the effect of administration of Levosimendan and Exenatide in various concentrations, as well as of the coadministration of those agents in an ischemia–reperfusion injury isolated heart model. (2) Methods: After 30 min of perfusion, the hearts underwent a 30 min period of regional ischemia followed by a 120 min period of reperfusion. All animals were randomly divided into 12 experimental groups of nine animals in each group: (1) Control, (2) Sham, (3) Digox (Negative control, Digoxin 1.67 μg/min), (4) Levo 1 (Levosimendan 0.01 μg/min), (5) Levo 2 (Levosimendan 0.03 μg/mL), (6) Levo 3 (Levosimendan 0.1 μg/min), (7) Levo 4 (Levosimendan 0.3 μg/min), (8) Levo 5 (Levosimendan 1 μg/min), (9) Exen 1 (Exenatide 0.001 μg/min), (10) Exen 2 (Exenatide 0.01 μg/min), (11) Exen 3 (Exenatide 0.1 μg/min) and (12) Combi (Levosimendan 0.1 µg/mL + Exenatide 0.001 μg/min). The hemodynamic parameters were recorded throughout the experiment. Arrhythmias and coronary flow were also evaluated. After every experiment the heart was suitably prepared and infarct size was measured. Markers of myocardial injury were also measured. Finally, oxidative stress was evaluated measuring reactive oxygen species. (3) Results: A dose-dependent improvement of the haemodynamic response was observed after the administration of both Levosimendan and Exenatide. The coadministration of both agents presented an even greater effect, improving the haemodynamic parameters further than the two agents separately. Levosimendan offered an increase of the coronary flow and both agents offered a reduction of arrhythmias. A dose-dependent reduction of the size of myocardial infarction and myocardial injury was observed after administration of Levosimendan and Exenatide. The coadministration of both agents offered a further improving the above parameters. Levosimendan also offered a significant reduction of oxidative stress. (4) Conclusions: The administration of Levosimendan and Exenatide offers a significant benefit by improving the haemodynamic response, increasing the coronary flow and reducing the occurrence of arrhythmias, the size of myocardial injury and myocardial oxidative stress in isolated rat hearts.

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Myofibrillar creatine kinase and cardiac contraction

Ventura‐Clapier¹,

Veksler²,

Hoerter³

1994

Cellular Bioenergetics: Role of Coupled Creatine Kinases

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This article is a review on the organization and function of myofibrillar creatine kinase in striated muscle. The first part describes myofibrillar creatine kinase as an integral structural part of the complex organization of myofibrils in striated muscle. The second part considers the intrinsic biochemical and mechanical properties of myofibrils and the functional coupling between myofibrillar CK and myosin ATPase. Skinned fiber studies have been developed to evidence this functional coupling and the consequences for cardiac contraction. The data show that creatine kinase in myofibrils is effective enough to sustain normal tension and relaxation, normal Ca sensitivity and kinetic characteristics. Moreover, the results suggest that myofibrillar creatine kinase is essential in maintaining adequate ATP/ADP ratio in the vicinity of myosin ATPase active site to prevent dysfunctioning of this enzyme. Implications for the physiology and physiopathology of cardiac muscle are discussed. (Mol Cell Biochem 133/134: 125-144, 1994)

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Contractile properties and creatine kinase activity of myofilaments following ischemia and reperfusion of the rat heart

Cited by 13 publications

References 20 publications

Creatine and Creatinine Metabolism

Creatine and Creatinine Metabolism

The Coadministration of Levosimendan and Exenatide Offers a Significant Cardioprotective Effect to Isolated Rat Hearts against Ischemia/Reperfusion Injury

Myofibrillar creatine kinase and cardiac contraction

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