Contractile mechanisms coupled to TRPA1 receptor activation in rat urinary bladder

Andrade, Edinéia Lemos de; Ferreira, Juliano; André, Eunice; Calixto, João B.

doi:10.1016/j.bcp.2006.04.003

Cited by 99 publications

(76 citation statements)

References 35 publications

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“…In Cl Ϫ -free solution, basal G t , but not I sc , was decreased, from 11.2 Ϯ 1.5 to 7.8 Ϯ 0.6 mS/cm 2 (P Ͻ 0.05, n ϭ 5). In Cl Ϫ -and HCO 3 Ϫ -free solution, basal I sc , but not G t , was decreased, from 20.1 Ϯ 3.8 to 2.6 Ϯ 3.4 A/cm 2 (P Ͻ 0.001, n ϭ 4). AITC-evoked increases in I sc and G t were significantly decreased in the absence of Cl Ϫ and were abolished in the absence of both Cl Ϫ and HCO 3 Ϫ (Table 3).…”

Section: ϫ5mentioning

confidence: 94%

Activation of TRPA1 by luminal stimuli induces EP₄-mediated anion secretion in human and rat colon

Kaji

Yasuoka

Karaki

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Kaji I, Yasuoka Y, Karaki S, Kuwahara A. Activation of TRPA1 by luminal stimuli induces EP4-mediated anion secretion in human and rat colon. Am J Physiol Gastrointest Liver Physiol 302: G690-G701, 2012. First published December 29, 2011; doi:10.1152/ajpgi.00289.2011 physiology, anion and fluid secretion is an important function for host defense and is induced by changes in the luminal environment. The transient receptor potential A1 (TRPA1) channel is considered to be a chemosensor in several sensory tissues. Although the function of TRPA1 has been studied in GI motility, its contribution to the transepithelial ion transport system has rarely been discussed. In the present study, we investigated the secretory effect of the potential TRPA1 agonist allyl isothiocyanate (AITC) in rat and human colon using an Ussing chamber. The mucosal application of AITC (10 Ϫ6 -10 Ϫ3 M) induced Cl Ϫ and HCO 3 Ϫ secretion in a concentrationdependent manner, whereas the serosal application induced a significantly weaker effect. AITC-evoked anion secretion was attenuated by tissue pretreatment with piroxicam and prostaglandin (PG) E2; however, this secretion was not affected by TTX, atropine, or extracellular Ca 2ϩ depletion. These experiments indicate that TRPA1 activation induces anion secretion through PG synthesis, independent of neural pathways in the colon. Further analysis also indicates that AITCevoked anion secretion is mediated mainly by the EP4 receptor subtype. The magnitude of the secretory response exhibited segmental heterogeneity in rat colon. Real-time PCR analysis showed the segmental difference was corresponding to the differential expression of EP4 receptor and cyclooxygenase-1 and -2. In addition, RT-PCR, in situ hybridization, and immunohistochemical studies showed TRPA1 expression in the colonic epithelia. Therefore, we conclude that the activation of TRPA1 in colonic epithelial cells is likely involved in the host defense mechanism through rapid anion secretion. colonic chemosensing; prostaglandin E2 THE EPITHELIUM OF THE GASTROINTESTINAL (GI) tract functions not only as a gate for water and nutrients but as a barrier between the luminal and internal environments. Recent studies have suggested that luminal sensory molecules in the small intestine influence nutrient metabolism and local physiological responses (1, 31, 32). Molecular identification of sensory receptors, such as olfactory, taste, and thermo-receptors, which are part of the transient receptor potential (TRP) family, has helped to elucidate chemical-sensing systems that detect the luminal state. However, the physiological roles of these receptors in colonic ion transport have rarely been investigated at the organ level. In particular, transepithelial anion secretion followed by fluid secretion is regulated by diverse systems, including the nervous, endocrine, and immune systems (4, 17), and is considered an important function for host defense. Therefore, experiments at the tissue or organ levels are important for understanding the contribution of ...

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Section: ϫ5mentioning

confidence: 94%

Activation of TRPA1 by luminal stimuli induces EP₄-mediated anion secretion in human and rat colon

Kaji

Yasuoka

Karaki

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…In addition, TRPA1 are present in sensory terminals of nociceptive fibers in their target organs like trigone of the bladder and the cornea [9,10]. Interestingly, TRPA1 channels are expressed on the protein level in several non-neuronal human tissues including gastrointestinal mucosa of small intestine and colon [11].…”

Section: Introductionmentioning

confidence: 99%

TRPA1 channel activation induces cholecystokinin release via extracellular calcium

et al. 2007

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TRPA1 channels are non-selective cation channels activated by plant derived pungent products including allyl isothiocyanate (AITC) from mustard. Therefore, possible intestinal secretory functions of these channels were investigated. We detected TRPA1 mRNA in mouse and human duodenal mucosa and in intestinal mouse neuroendocrine STC-1 cells. Stimulation of STC-1 cells with AITC increased intracellular calcium ([Ca 2+ ] i ) and significantly stimulated cholecystokinin secretion by 6.7-fold. AITC induced cholecystokinin release was completely blocked by TRPA1 antagonist ruthenium red and depletion of extracellular calcium and reduced by 36% by nimodipine and nifedipine. This suggests that spices in our daily food might stimulate digestive functions.

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“…Expression of other TRP channels, e.g., TRPM8 and TRPA1, is found in sensory C fibers in the bladder (27,28). TRPM8 forms the basis of the diagnostic ice water test to determine whether disturbance of bladder function involves a neurogenic component (29).…”

Section: Introductionmentioning

confidence: 99%

Deletion of the transient receptor potential cation channel TRPV4 impairs murine bladder voiding

Gevaert¹,

Vriens²,

Segal³

et al. 2007

J. Clin. Invest.

289

349

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Here we provide evidence for a critical role of the transient receptor potential cation channel, subfamily V, member 4 (TRPV4) in normal bladder function. Immunofluorescence demonstrated TRPV4 expression in mouse and rat urothelium and vascular endothelium, but not in other cell types of the bladder. Intracellular Ca 2+ measurements on urothelial cells isolated from mice revealed a TRPV4-dependent response to the selective TRPV4 agonist 4α-phorbol 12,13-didecanoate and to hypotonic cell swelling. Behavioral studies demonstrated that TRPV4 -/-mice manifest an incontinent phenotype but show normal exploratory activity and anxietyrelated behavior. Cystometric experiments revealed that TRPV4 -/-mice exhibit a lower frequency of voiding contractions as well as a higher frequency of nonvoiding contractions. Additionally, the amplitude of the spontaneous contractions in explanted bladder strips from TRPV4 -/-mice was significantly reduced. Finally, a decreased intravesical stretch-evoked ATP release was found in isolated whole bladders from TRPV4 -/-mice. These data demonstrate a previously unrecognized role for TRPV4 in voiding behavior, raising the possibility that TRPV4 plays a critical role in urothelium-mediated transduction of intravesical mechanical pressure.

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Contractile mechanisms coupled to TRPA1 receptor activation in rat urinary bladder

Cited by 99 publications

References 35 publications

Activation of TRPA1 by luminal stimuli induces EP₄-mediated anion secretion in human and rat colon

Activation of TRPA1 by luminal stimuli induces EP₄-mediated anion secretion in human and rat colon

TRPA1 channel activation induces cholecystokinin release via extracellular calcium

Deletion of the transient receptor potential cation channel TRPV4 impairs murine bladder voiding

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