Contractile behaviors of cardiac muscle cells on mushroom-shaped micropillar arrays

Oyunbaatar, Nomin-Erdene; Shanmugasundaram, Arunkumar; Lee, Dong-Weon

doi:10.1016/j.colsurfb.2018.10.058

Cited by 23 publications

(15 citation statements)

References 22 publications

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“…To prevent the nonspecific binding of the antibodies, the sample for nuclei staining was conducted at 37°C for 15min. Immunocytochemical staining analysis was quantitatively performed using an ImageJ software with a normalization from the level of the entire protein [3].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a variety of methods have been reported for assessing drug toxicity by measuring the contractile force of cardiac cells in in vitro environments [1][2][3][4][5][6][7][8][9]. Methods for the direct measurement of the contractile force include image processing analysis using an optical microscope and laser-based displacement sensors.…”

Section: Introductionmentioning

confidence: 99%

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Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility

Kim¹,

Choi²,

Jeong³

et al. 2020

Nat Commun

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We propose a novel cantilever device integrated with a polydimethylsiloxane (PDMS)encapsulated crack sensor that directly measures the cardiac contractility. The crack sensor was chemically bonded to a PDMS thin layer to form a sandwiched structure which allows to be operated very stably in culture media. The reliability of the proposed crack sensor has improved dramatically -2-compared to no encapsulation layer. After evaluating the durability of the crack sensor bonded with the PDMS layer, cardiomyocytes were cultured on the nano-patterned cantilever for real-time measurement of cardiac contractile forces. The highly sensitive crack sensor continuously measured the cardiac contractility without changing its gauge factor for up to 26 days (>5 million heartbeats). In addition, changes in contractile force induced by drugs were monitored using the crack sensorintegrated cantilever. Finally, experimental results were compared with those obtained via conventional electrophysiological methods to verify the feasibility of building a contraction-based drug-toxicity testing system.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility

Kim¹,

Choi²,

Jeong³

et al. 2020

Nat Commun

Self Cite

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“…Some researchers used micropillars to measure the contraction force. Cells cultured on the micropillars tend to adhere to the tips[ 60 ]. When the cell contracts, it would deflect the surrounding micropillars ( Figure 3Aii )[ 61 ].…”

Section: The Structures Of Heart-on-a-chipmentioning

confidence: 99%

Fabrication and Biomedical Applications of Heart-on-a-chip

Yang

Xiao

et al. 2024

IJB

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Heart diseases have become the main killer threatening human health, and various methods have been developed to study heart disease. Among them, heart-on-a-chip has emerged in recent years as a method for constructing disease (or normal) models in vitro and is considered as a promising tool to study heart diseases. Compared with other methods, the advantages of heart-on-a-chip include the high portability, high throughput, and the capability to mimic microenvironments in vivo. It has shown a great potential in disease mechanism study and drug screening. In this paper, we review the recent advances in heart on-a-chip, including the fabrication methods (e.g., 3D bioprinting) and biomedical applications. By analyzing the structure of the existing heart-on-a-chip, we proposed that a highly integrated heart-on-a-chip includes four elements: Microfluidic chips, cells/microtissues, microactuators to construct the microenvironment, and microsensors for results readout. Finally, the current challenges and future directions of heart-on-a-chip are discussed.

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“…Next, they also developed a mushroom-shaped PDMS MPAs ( Figure 5C), which exhibited higher contractile force (20%) compared to conventional PDMS MPAs due to special topographical dependency. [129] Though MPAs can measure the contractile force of cardiomyocytes by monitoring the bending of elastomeric micropillars, this method also suffers from some troubles. For example, the hard contact between cells and micropillars may affect the functions of cell membranes and cardiomyocytes.…”

Section: Micropillar Arrays (Mpas)mentioning

confidence: 99%

“…Next, they also developed a mushroom‐shaped PDMS MPAs (Figure 5C), which exhibited higher contractile force (20%) compared to conventional PDMS MPAs due to special topographical dependency. [ 129 ]…”

Section: Contractile Force Detectionmentioning

confidence: 99%

Advances in Multidimensional Cardiac Biosensing Technologies: From Electrophysiology to Mechanical Motion and Contractile Force

Wei

Zhuang

et al. 2020

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Cardiovascular disease (CVD) is the number one killer in the world. According to the World Health Organization (WHO) estimation, about 17.9 million people died from CVD each year, accounting for 31% of all deaths worldwide. [1] Among these deaths, about 7.4 million died from coronary heart disease, while 6.7 million from a stroke. According to China cardiovascular disease report in 2018, [2] around 290 million people suffer from CVD, including 13 million strokes, 11 million coronary heart disease, 5 million pulmonary heart disease, 4.5 million heart failure, 2.5 million rheumatic heart disease, 2 million congenital heart disease, and 270 million hypertension. Cardiovascular disease was also the leading cause of all death in rural and urban areas, with 45.01% in rural areas and 42.61% in urban areas. According to heart disease and stroke statistics from the American Heart Association (AHA) in 2018, [3] around 92.1 million American adults have a certain form of cardiovascular disease or suffer from stroke sequelae. CVD killed 836546 Americans in 2015. Coronary heart disease was the leading cause (43.8%) of death in CVD, followed by stroke (16.8%), heart failure (9.0%), hypertension (9.4%), arterial disease (3.1%) and other cardiovascular diseases (17.9%). According to the European cardiovascular disease statistics in 2017, [4] around 3.9 million people died from CVD each year, which accounts for 45% of all deaths in Europe. Ischemic heart disease (IHD) and stroke are the main forms of CVD in Europe. Among all deaths in Europe each year, IHD is the leading single cause of mortality, responsible for 19% and 20% among men and women, while stroke is the second most common single cause of death, accounting for 9% in men and 13% in women. Therefore, the prevention and treatment of CVD have aroused extensive public concern worldwide. Moreover, cardiac toxicity induced by drugs has been the main cause of the withdrawal from the market and drugs attrition during the last decades. [5,6] On the one hand, drug-induced cardiotoxicity will also threaten people's health, even life. For example, acute arrhythmia can lead to sudden death, while Cardiovascular disease is currently a leading killer to human, while druginduced cardiotoxicity remains the main cause of the withdrawal and attrition of drugs. Taking clinical correlation and throughput into account, cardiomyocyte is perfect as in vitro cardiac model for heart disease modeling, drug discovery, and cardiotoxicity assessment by accurately measuring the physiological multiparameters of cardiomyocytes. Remarkably, cardiomyocytes present both electrophysiological and biomechanical characteristics due to the unique excitation-contraction coupling, which plays a significant role in studying the cardiomyocytes. This review mainly focuses on the recent advances of biosensing technologies for the 2D and 3D cardiac models with three special properties: electrophysiology, mechanical motion, and contractile force. These high-performance multidimensional cardiac models are popular and ef...

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Contractile behaviors of cardiac muscle cells on mushroom-shaped micropillar arrays

Cited by 23 publications

References 22 publications

Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility

Highly durable crack sensor integrated with silicone rubber cantilever for measuring cardiac contractility

Fabrication and Biomedical Applications of Heart-on-a-chip

Advances in Multidimensional Cardiac Biosensing Technologies: From Electrophysiology to Mechanical Motion and Contractile Force

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