Continuum model of mechanical interactions between biological cells and artificial nanostructures

Verma, Piyush; Wong, Ian Y.; Melosh, Nicholas A.

doi:10.1116/1.3431960

Cited by 18 publications

(21 citation statements)

References 37 publications

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“…6)3451525657. It is conceivable that functionalization of the gMμEs with alkanethiols might facilitate the formation of a stable high seal resistance junction between the electrode and the cell’s plasma membrane58596061. Under such conditions the gMμE may be in direct contact with the neuron’s cytosol for long periods of time525862.…”

Section: Discussionmentioning

confidence: 99%

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Rabieh

Ojovan

Shmoel

et al. 2016

Sci Rep

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In contrast to the extensive use of microelectrode array (MEA) technology in electrophysiological studies of cultured neurons and cardiac muscles, the vast field of skeletal muscle research has yet to adopt the technology. Here we demonstrate an empowering MEA technology for high quality, multisite, long-term electrophysiological recordings from cultured skeletal myotubes. Individual rat skeletal myotubes cultured on micrometer sized gold mushroom-shaped microelectrode (gMμE) based MEA tightly engulf the gMμEs, forming a high seal resistance between the myotubes and the gMμEs. As a consequence, spontaneous action potentials generated by the contracting myotubes are recorded as extracellular field potentials with amplitudes of up to 10 mV for over 14 days. Application of a 10 ms, 0.5–0.9 V voltage pulse through the gMμEs electroporated the myotube membrane, and transiently converted the extracellular to intracellular recording mode for 10–30 min. In a fraction of the cultures stable attenuated intracellular recordings were spontaneously produced. In these cases or after electroporation, subthreshold spontaneous potentials were also recorded. The introduction of the gMμE-MEA as a simple-to-use, high-quality electrophysiological tool together with the progress made in the use of cultured human myotubes opens up new venues for basic and clinical skeletal muscle research, preclinical drug screening, and personalized medicine.

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Section: Discussionmentioning

confidence: 99%

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

Rabieh

Ojovan

Shmoel

et al. 2016

Sci Rep

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“…Spontaneous membrane penetration by high‐aspect‐ratio nanostructures is perhaps the largest discussion area in the field of high‐aspect‐ratio nanostructures and hence multiple attempts have been made to model this interaction. Melosh and co‐workers developed a continuum‐based model for cell–nanowire interactions . Their model is based on balancing the gravitational force acting upon the cell, with the hydrostatic pressure inside (considering the cell as a membrane bound liquid) and the net membrane tension.…”

Section: Modeling the Cell–nanostructure Interfacementioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

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“…Unfortunately, the diameter of this artificial transmembrane link is limited to a few hundred nanometers due to the nature of the penetration mechanism7. This size is too small to deliver many biomolecules or safely inject large amount of electrical charge into the cells8.…”

mentioning

confidence: 99%

Biomimetic surface patterning for long-term transmembrane access

VanDersarl

Renaud

2016

Sci Rep

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Here we present a planar patch clamp chip based on biomimetic cell membrane fusion. This architecture uses nanometer length-scale surface patterning to replicate the structure and function of membrane proteins, creating a gigaohm seal between the cell and a planar electrode array. The seal is generated passively during cell spreading, without the application of a vacuum to the cell surface. This interface can enable cell-attached and whole-cell recordings that are stable to 72 hours, and generates no visible damage to the cell. The electrodes can be very small (<5 μm) and closely packed, offering a high density platform for cellular measurement.

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Continuum model of mechanical interactions between biological cells and artificial nanostructures

Cited by 18 publications

References 37 publications

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Biomimetic surface patterning for long-term transmembrane access

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