Continuous versus batch production of lipids in the microalgae Acutodesmus obliquus

Remmers, Ilse M.; Hidalgo-Ulloa, Adrian; Brandt, Boudewijn P.; Evers, Wendy A. C.; Wijffels, René H.; Lamers, Packo P.

doi:10.1016/j.biortech.2017.04.093

Cited by 47 publications

(59 citation statements)

References 48 publications

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“…However, TAG productivity R TAG increased with increasing MRPA A XA when expressed per kg of active biomass X A indicating that TAG production rate was certainly limited by a reaction governed by light absorption. In this study, maximum TAG productivity reached 2.6 g/m 2 ⋅day for the culture featuring MRPA equal to 17,700 μmol hν /kg XA s. This is similar to maximum TAG productivity of 2.2 g/m 2 ⋅day reported by Remmers et al [10] for the wild type A. obliquus grown in continuous mode with a mean rate of photon absorption estimated to be 13,080 μmol hν /kg XA s. The latter was determined assuming a steady-state biomass and TAG concentrations of 2.45 kg/m 3 (with active biomass fraction 0.52) and 22.1 dry wt%, respectively, and full absorption of the 333 μmol hv /m 2 ⋅s incident on the PBR. The similarity of both maximum TAG productivity and its corresponding MRPA reported here and in Ref.…”

Section: Mean Specific Rate Of Photon Absorptionsupporting

confidence: 90%

“…The similarity of both maximum TAG productivity and its corresponding MRPA reported here and in Ref. [10] provides further confidence in the use of MRPA expressed per kg of active biomass to optimize TAG productivity in nitrogen limited cultures. Kandilian et al [32] previously demonstrated TAG productivity in nitrogen starved Nannochloropsis oculata and MRPA had a parabolic relation where maximum productivity occurred at 14 μmol hv /m 2 ⋅s.…”

Section: Mean Specific Rate Of Photon Absorptionsupporting

confidence: 79%

“…To achieve nitrogen limited growth in chemostat mode a small amount of nitrate is continuously added to the PBR such that the steady-state nitrate concentration in the PBR is very small so as to induce TAG production. Recently, Remmers et al [10] cultivated wild type and starchless mutant strains of Acutodesmus obliquus in nitrogen limited medium in batch and continuous (turbidostat) modes. The authors exposed a 2 cm thick airlift PBR containing 1.7 L of microalgae culture to a photon flux density of 500 μmol hν /m 2 ⋅s at 16:8 hour light/ dark cycles.…”

Section: Introductionmentioning

confidence: 99%

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Effect of light absorption rate and nitrate concentration on TAG accumulation and productivity of Parachlorella kessleri cultures grown in chemostat mode

et al. 2019

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This study aims to elucidate the role of light absorption rate and nitrate concentration on triacylglycerol (TAG) productivity and cellular TAG accumulation in nitrogen limited Parachlorella kessleri cultures grown in chemostat mode. In batch operated nitrogen starved cultures the cells accumulate large amounts of TAGs at the expense of biomass growth leading to severe decrease in cell division rate prompting eventual cell death. In PBRs operated in continuous mode cell multiplication can persist in nitrogen limited conditions with simultaneous TAG accumulation. P. kessleri cultures were grown in a flat-plate PBR with constant dilution rate of 0.01 1/h and feed-medium nitrate concentration ranging from 1 to 16 mM. The steady-state biomass, pigment, carbohydrate, protein, total lipid, and TAG concentrations as well as the spectral absorption and scattering cross-sections of the microalgae were measured for each culture. These were used to estimate the specific mean rate of photon absorption (MRPA) in the PBR. Maximum areal TAG productivity of 2.6 g/m 2 ⋅day was obtained for the culture grown with nitrate concentration equal to 3.65 mM and a mean rate of photon absorption equal to 17,700 μmol hν /kg X A s. TAG productivity and cellular TAG accumulation varied with the nitrate concentration in the feed medium and MRPA. Finally, our results show that TAG productivity can be further increased by optimizing the mean rate of photon absorption through adjusting culture dilution rate and feed-medium nitrate concentration.

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Section: Mean Specific Rate Of Photon Absorptionsupporting

confidence: 90%

Section: Mean Specific Rate Of Photon Absorptionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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Effect of light absorption rate and nitrate concentration on TAG accumulation and productivity of Parachlorella kessleri cultures grown in chemostat mode

et al. 2019

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“…Due to the expanding biobased economy, the interest in microbial oil is increasing (Athenaki et al 2018;Sitepu et al 2014a, b). The possibility of economically feasible bio-oil production is being investigated in algae, yeasts, and filamentous fungi (Remmers et al 2017;Hao et al 2016;Sitepu et al 2014b;Ageitos et al 2011). However, an economically viable production process will require improvements to oil yield through optimization of cultivation and processing, as well as isolation of multiple coproducts in a biorefinery setup, indicating the need for mild downstream processing techniques.…”

Section: Introductionmentioning

confidence: 99%

Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

et al. 2020

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Due to the depletion of fossil fuel resources and concern about increasing atmospheric CO 2 levels, the production of microbial oil as source for energy and chemicals is considered as a sustainable alternative. A promising candidate strain for the production of microbial oil is the oleaginous yeast Schwanniomyces occidentalis CBS 2864. To compete with fossil resources, cultivation and processing of S. occidentalis requires improvement. Currently, different cell wall disruption techniques based on mechanical, chemical, physiological, and biological methods are being investigated using a variety of oil producing yeasts and microalgae. Most of these techniques are not suitable for upscaling because they are technically or energetically unfavorable. Therefore, new techniques have to be developed to overcome this challenge. Here, we demonstrate an effective mild enzymatic approach for cell disruption to facilitate lipid extraction from the oleaginous yeast S. occidentalis. Most oil was released by applying 187 mg L −1 tailor-made enzymes from Trichoderma harzianum CBS 146429 against the yeast cell wall of S. occidentalis at pH 5.0 and 40 °C with 4 h of incubation time after applying 1 M NaOH as a pretreatment step.

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“…14 Acutodesmus obliquus (Scenedesmus obliquus UTEX 393) was chosen for this research due to its biomass potential for biodiesel production as well as its greater potential for use as lipid producer, all these attributes being due to its tolerance to pH variation and easy culturing when nitrogen starvation (stress phase) is involved. 15,16 www.soci.org DX Hurtado et al…”

Section: Introductionmentioning

confidence: 99%

Using different wavelengths and irradiance on the microalgae Acutodesmus obliquus batch culture

Hurtado

Garzón‐Castro

Cortés‐Romero

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The quality of light, such as wavelength and the intensity affect the performance of algal growth and lipid production. Moreover, these variables also affect the lipids profile. To study the interrelation among these variables, Acutodesmus obliquus (Scenedesmus obliquus UTEX393) a microalga, was cultivated using illumination by light-emitting-diode surface mount device extensions emitting red ( = 620-750 nm), yellow ( = 570-590 nm), green ( = 495-570 nm) and violet ( = 380-450 nm) light. Additionally, each culture was illuminated at different light intensities (I 1 = 8.75, I 2 = 14.22 and I 3 = 19.69 W m −2 ). Finally, all samples were analysed by GC-MS. RESULTS:The results showed: (1) the biomass production is directly proportional to the light intensity, e.g. at I 3 = 19.69 W m −2 violet (36.80 mg L −1 ), yellow (25.49 mg L −1 ) and red (39.44 mg L −1 ) showed the major production; (2) however, the lipids were produced in minor amounts at major violet light intensity (3.64 mg L −1 at I 3 = 19.69 W m −2 ); (3) the major lipids production (20.56 mg L −1 ) was obtained with red light at I 3 = 19.69 W m −2 and (4) A. obliquus, under the parameters established was a good producer of lipids. CONCLUSIONS:The major amount of biomass was obtained at red, yellow and violet light; meanwhile, the low biomass production, using green light, was associated with inefficient absorption of green light by chlorophyll. Additionally, palmitic, linolenic and linoleic were the most produced lipids, establishing a dependence between light wavelength and irradiance with biomass and lipid production. These results support the use of wavelengths and intensities to produce a group of specific lipids according to the needs of different industries.

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Continuous versus batch production of lipids in the microalgae Acutodesmus obliquus

Cited by 47 publications

References 48 publications

Effect of light absorption rate and nitrate concentration on TAG accumulation and productivity of Parachlorella kessleri cultures grown in chemostat mode

Effect of light absorption rate and nitrate concentration on TAG accumulation and productivity of Parachlorella kessleri cultures grown in chemostat mode

Production of tailor-made enzymes to facilitate lipid extraction from the oleaginous yeast Schwanniomyces occidentalis

Using different wavelengths and irradiance on the microalgae Acutodesmus obliquus batch culture

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