Continuous size-based DEP separation of particles using a bi-gap electrode pair

Derakhshan, Reza; Ramiar, Abas; Ghasemi, Amirhosein

doi:10.1039/d2an01308h

Cited by 5 publications

(1 citation statement)

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“…DEP‐based separation methods such as field‐flow fractionation (FFF) and lateral fluid flow fractionation (LFFF) have been developed for the separation of particles and cells. FFF uses an array of interdigitated microelectrodes to generate dielectrophoretic forces that levitate the bioparticle mixture, allowing for controlled spatial separation based on the balance between DEP levitation and gravitational forces [14]. On the other hand, LFFF is a continuous‐flow microdevice used for the detection and isolation of CTCs in the presence of other hematological cells [15].…”

Section: Introductionmentioning

confidence: 99%

Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer‐based surface modification

Lin,

Su,

Huang

et al. 2024

Electrophoresis

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Metastasis remains a significant cause to cancer‐related mortality, underscoring the critical need for early detection and analysis of circulating tumor cells (CTCs). This study presents a novel microfluidic chip designed to efficiently capture A549 lung cancer cells by combining dielectrophoresis (DEP) and aptamer‐based binding, thereby enhancing capture efficiency and specificity. The microchip features interdigitated electrodes made of indium‐tin‐oxide that generate a nonuniform electric field to manipulate CTCs. Following three chip design, scenarios were investigated: (A) bare glass surface, (B) glass modified with gold nanoparticles (AuNPs) only, and (C) glass modified with both AuNPs and aptamers. Experimental results demonstrate that AuNPs significantly enhance capture efficiency under DEP, with scenarios (B) and (C) exhibiting similar performance. Notably, scenario (C) stands out as aptamer‐functionalized surfaces resisting fluid shear forces, achieving CTCs retention even after electric field deactivation. Additionally, an innovative reverse pumping method mitigates inlet clogging, enhancing experimental efficiency. This research offers valuable insights into optimizing surface modifications and understanding key factors influencing cell capture, contributing to the development of efficient cell manipulation techniques with potential applications in cancer research and personalized treatment options.

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Section: Introductionmentioning

confidence: 99%