Continuous separation of fungal spores in a microfluidic flow focusing device

Park, Byeong Seon; Kye, Hyeon Gi; Kim, Tae Hyeon; Lee, Jong Min; Ahrberg, Christian D.; Cho, Eun-Min; Yang, Sung Ik; Chung, Bong Geun

doi:10.1039/c9an00905a

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…In the Newtonian fluid or the viscoelastic fluid with high inertial effect, the inertial lift force greatly affects the lateral migration of the particle. Fi can be estimated as follows (Lu et al 2017;Yuan et al 2018;Park et al 2019):…”

Section: Theoretical Background and Working Mechanismmentioning

confidence: 99%

Continuous sheathless particle separation in viscoelastic fluids with different rheological properties

Gan,

Tian,

Liu

et al. 2024

Microfluid Nanofluid

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The separation of particles such as cells and bacteria in viscoelastic fluids has significant applications in biomedical fields. At present, one of the main challenges that limit the application of microfluidic technology is to separate particles in the viscoelastic fluids with different rheological properties. For instance, most exisiting microfluidic devices can only work in the fluid with a specific rheological property, resulting in the requirement of time-consuming design, manufacturing, testing, and optimization of different devices to separate particles in the fluids with different rheological properties. In this work, a new particle separation strategy that is first focusing, then initial separation, and then precise separation (FISPS) was proposed, and a novel hybrid three-stage microfluidic device where a micropore structure was utilized to connect two gradually contracted microchannels was designed to achieve efficient continuous separation of particles in the viscoelastic fluid over a wide range of rheological properties (0.07 < El < 340.41). The device showed a separation mechanism of initial separation and then precise separation (ISPS) at El < 0.14 while it showed the separation mechanism of first focusing, then initial separation, and then precise separation (FISPS) at El > 8.43. In addition, the transformation of the separation mechanism from ISPS to FISPS was found under different flow conditions in the fluid with the transitional rheological properties (0.21 < El < 1.10). The effect of the flow rate and the rheological property of the fluid on microparticle separation were systematically studied by the experiment. With simple structure, easy operation, high separation efficiency, the present microfluidic device would have great potentials in the biomedical and clinical applications, such as the separation of cells for different patients.

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Section: Theoretical Background and Working Mechanismmentioning

confidence: 99%

Continuous sheathless particle separation in viscoelastic fluids with different rheological properties

Gan,

Tian,

Liu

et al. 2024

Microfluid Nanofluid

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“…This device integrates IMF and CFF. h PFF/BFF (branch flow fractionation) fungal spore passive sorting device 17 . i Inertia-enhanced PFF for microalgae separation 198 .…”

Section: Combination Of Different Geometric Structuresmentioning

confidence: 99%

“…Park et al proposed another structure that integrates PFF and BFF (branch flow fractionation) (Fig. 10h ) 17 . Their device was used for the passive sorting of fungal spores.…”

Section: Combination Of Different Geometric Structuresmentioning

confidence: 99%

“…Microfluidic separation devices have established their reputation based on a reduced sample and reagent volumes, improved portability, significant sensitivity, and low cost 6 . Minute bioparticles, such as red blood cells (RBCs) and white blood cells (WBCs) 7 , CTCs 2 , 8 – 10 , exosomes 11 , 12 , DNA 13 , parasites 14 , bacteria 15 , 16 , and spores 5 , 17 , 18 , can be separated by microfluidics based on their size differences and other attributes. There are two types of microfluidic separation devices: active and passive 19 .…”

Section: Introductionmentioning

confidence: 99%

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Geometric structure design of passive label-free microfluidic systems for biological micro-object separation

et al. 2022

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Passive and label-free microfluidic devices have no complex external accessories or detection-interfering label particles. These devices are now widely used in medical and bioresearch applications, including cell focusing and cell separation. Geometric structure plays the most essential role when designing a passive and label-free microfluidic chip. An exquisitely designed geometric structure can change particle trajectories and improve chip performance. However, the geometric design principles of passive and label-free microfluidics have not been comprehensively acknowledged. Here, we review the geometric innovations of several microfluidic schemes, including deterministic lateral displacement (DLD), inertial microfluidics (IMF), and viscoelastic microfluidics (VEM), and summarize the most creative innovations and design principles of passive and label-free microfluidics. We aim to provide a guideline for researchers who have an interest in geometric innovations of passive label-free microfluidics.

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“…These methods can be broadly categorized as either passive or active. The passive methods, including microfiltration 3 , inertia-based 4 , contraction–expansion channels 5 , deterministic lateral displacement 6 , and pinched flow fractionation 7 , do not rely on any external forces. On the other hand, the active methods, such as dielectrophoresis (DEP) 8 , magnetophoresis 9 , acoustophoresis 10 , and optical-based manipulation 11 , require an external force to manipulate the cells/particles.…”

Section: Introductionmentioning

confidence: 99%

Parametric study on the geometrical parameters of a lab-on-a-chip platform with tilted planar electrodes for continuous dielectrophoretic manipulation of microparticles

Dalili

Taatizadeh

Tahmooressi

et al. 2020

Sci Rep

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Advances in lab-on-a-chip (LOC) devices have led to significant improvements in the on-chip manipulation, separation, sorting, and isolation of particles and cells. Among various Loc-based approaches such as inertia-based methods, acoustophoresis, and magnetophoresis, the planarslanted-electrode dielectrophoresis (Dep) method has demonstrated great potential as a label-free, cost-effective, and user-friendly approach. However, the devices built based on this method suffer from low flow throughput compared to devices functioning based on other LOC-based manipulation approaches. in order to overcome this obstacle, the geometrical parameters of these types of DEP-based devices must be studied to increase the effectiveness of DEP manipulation. With the consideration of both numerical and experimental studies, this paper studies the geometrical factors of a Loc platform consisting of tilted planar electrodes with the goal of achieving higher throughput in continuous manipulation of polystyrene particles. coMSoL Multiphysics software was used to study the effect of the electrodes geometry on the induced electric field. The simulation results show that by increasing the electrode's width and decreasing the electrode's spacing, higher Dep force is generated. furthermore, the experimental outcomes indicated that lower channel height, higher voltage, and larger particle size resulted in the most improvement to Dep manipulation. Additionally, the experimental results demonstrated that slanted electrodes with an angle of 8° with respect to the direction of flow provide a more effective configuration. Cell manipulation, as a preliminary step for cell-based analysis, is a rapidly growing area of interdisciplinary research for the development of single-cell technologies. Over the past two decades, single-cell manipulation and analysis methods have improved significantly due to advances in microfluidic cell manipulation methods 1,2. These methods can be broadly categorized as either passive or active. The passive methods, including microfiltration 3 , inertia-based 4 , contraction-expansion channels 5 , deterministic lateral displacement 6 , and pinched flow fractionation 7 , do not rely on any external forces. On the other hand, the active methods, such as dielectrophoresis (DEP) 8 , magnetophoresis 9 , acoustophoresis 10 , and optical-based manipulation 11 , require an external force to manipulate the cells/particles. Although passive methods can handle higher flow rates, active methods offer more control over cells/particles, real-time tuning, reliability, and higher manipulation efficiency 12-14 .

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Continuous separation of fungal spores in a microfluidic flow focusing device

Abstract: The research of fungi is of great importance in a number of fields, such as environmental and healthcare studies.

Cited by 9 publications

References 49 publications

Continuous sheathless particle separation in viscoelastic fluids with different rheological properties

Continuous sheathless particle separation in viscoelastic fluids with different rheological properties

Geometric structure design of passive label-free microfluidic systems for biological micro-object separation

Parametric study on the geometrical parameters of a lab-on-a-chip platform with tilted planar electrodes for continuous dielectrophoretic manipulation of microparticles

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