Continuous release of gentamicin from gold nanocarriers

Perni, Stefano; Prokopovich, Polina

doi:10.1039/c4ra10023a

Cited by 31 publications

(19 citation statements)

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“…Similar kinetic of release were reported from gentamicin conjugated onto the surface of functionalised gold nanoparticles through the formation of ester bonds [12]; this points towards the controlling role of the type of bond on the release kinetic of conjugated antibiotic on surfaces. The functionalisation of the particles had an extreme impact on the loading and desorption of gentamicin, when either amino (positively charged) or carboxyl (negatively charged) were present on the surface more antibiotic was adsorbed onto the surface and the release of gentamicin was almost complete compared to about 10% seen in unfunctionalised silica nanoparticles.…”

Section: Discussionsupporting

confidence: 52%

Controlling release kinetics of gentamicin from silica nano-carriers

Perni

Martini-Gilching

Prokopovich

2018

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Antibiotic release from a carrier can be employed to reduce the frequency of administration or prolong efficacy. In this work, we prepared silica nanoparticles loaded with gentamicin through different synthetis routes (entrapment, adsorption, covalent bonding and Layer-by-Layer (LbL) techniques). The nanocarriers physical-chemical properties were characterised and antibiotic release from the nanocarriers was monitored. The nanoparticles prepared entrapping gentamicin gave the highest drug load but completed the release over a period of 4 hours. No significant differences in antibiotic load were noticed between absorption or binding of gentamicin onto the silica nanoparticles surface; moreover the release of the drug occurred over 2 days. The nanoparticles coated with gentamicin through LbL technique released the antibiotic for 3 weeks. This work demonstrates that silica nanoparticles can be employed as antibiotic carriers providing a continuous release of antibiotic over a period that can be tuned through the choice of preparation method.

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Section: Discussionsupporting

confidence: 52%

Controlling release kinetics of gentamicin from silica nano-carriers

Perni

Martini-Gilching

Prokopovich

2018

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…The rate of this reaction increases further from neutrality, thus leading to the gradually greater release of DEX from pH =7 to pH =4. A similar drug release profile was observed by Nuttelman et al, 52 where DEX was covalently attached to polyethylene glycol-based hydrogel scaffold and for gentamicin when attached to gold 53 and silica. 49 As for mercapto route ( Figure 6B), results showed that DEX presented a less efficient, and with lower drug concentrations, release when compared with amino route.…”

Section: Dex Release Profilesupporting

confidence: 79%

<p>Anti-inflammatory drug-eluting implant model system to prevent wear particle-induced periprosthetic osteolysis</p>

Rivera¹,

Perni²,

Sloan³

et al. 2019

IJN

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BackgroundAseptic loosening, as a consequence of an extended inflammatory reaction induced by wear particles, has been classified as one of the most common complications of total joint replacement (TJR). Despite its high incidence, no therapeutical approach has yet been found to prevent aseptic loosening, leaving revision as only effective treatment. The local delivery of anti-inflammatory drugs to modulate wear-induced inflammation has been regarded as a potential therapeutical approach to prevent aseptic-loosening.MethodsIn this context, we developed and characterized anti-inflammatory drug-eluting TiO2 surfaces, using nanoparticles as a model for larger surfaces. The eluting surfaces were obtained by conjugating dexamethasone to carboxyl-functionalized TiO2 particles, obtained by using either silane agents with amino or mercapto moieties.ResultsZeta potential measurements, thermogravimetric analysis (TGA) and drug release results suggest that dexamethasone was successfully loaded onto the TiO2 particles. Release was pH dependent and greater amounts of drug were observed from amino route functionalized surfaces. The model-system was then tested for its cytotoxic and anti-inflammatory properties in LPS-stimulated macrophages. Dexamethasone released from amino route functionalized surfaces TiO2 particles was able to decrease LPS-induced nitric oxide (NO) and TNF-a production similarly to pure DEX at the same concentration; DEX released from mercapto route functionalized surfaces was at a too low concentration to be effective.ConclusionDexamethasone released from amino functionalized titanium can offer the possibility of preventing asepting loosening of joint replacement devices.

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“…The SiO 2 -gentamicin nanohybrids show a weight loss of 2.16% from 100 to ~220°C and a final weight loss (13.70%) from 220 to 500°C. A temperature of ~220°C can be considered as the beginning of gentamicin decomposition, 33 which continued as the temperature increased. The amount of gentamicin in the SiO 2 -gentamicin nanohybrids can be determined by subtracting the mass loss of native SiO 2 NPs from the mass loss of SiO 2 -gentamicin nanohybrids, after precluding the weight loss of water in both samples.…”

Section: Characterization Of the Sio 2 -Gentamicin Nanohybrids And Namentioning

confidence: 99%

Effects of silica–gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells

He¹,

Mosselhy²,

Zheng³

et al. 2018

IJN

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Introduction In recent years, there has been an increasing interest in silica (SiO 2 ) nanoparticles (NPs) as drug delivery systems. This interest is mainly attributed to the ease of their surface functionalization for drug loading. In orthopedic applications, gentamicin-loaded SiO 2 NPs (nanohybrids) are frequently utilized for their prolonged antibacterial effects. Therefore, the possible adverse effects of SiO 2 –gentamicin nanohybrids on osteogenesis of bone-related cells should be thoroughly investigated to ensure safe applications. Materials and methods The effects of SiO 2 –gentamicin nanohybrids on the cell viability and osteogenic differentiation of human osteoblast-like SaOS-2 cells were investigated, together with native SiO 2 NPs and free gentamicin. Results The results of Cell Count Kit-8 (CCK-8) assay show that both SiO 2 –gentamicin nanohybrids and native SiO 2 NPs reduce cell viability of SaOS-2 cells in a dose-dependent manner. Regarding osteogenesis, SiO 2 –gentamicin nanohybrids and native SiO 2 NPs at the concentration range of 31.25–125 μg/mL do not influence the osteogenic differentiation capacity of SaOS-2 cells. At a high concentration (250 μg/mL), both materials induce a lower expression of alkaline phosphatase (ALP) but an enhanced mineralization. Free gentamicin at concentrations of 6.26 and 9.65 μg/mL does not significantly influence the cell viability and osteogenic differentiation capacity of SaOS-2 cells. Conclusions The results of this study suggest that both SiO 2 –gentamicin nanohybrids and SiO 2 NPs show cytotoxic effects to SaOS-2 cells. Further investigation on the effects of SiO 2 –gentamicin nanohybrids on the behaviors of stem cells or other regular osteoblasts should be conducted to make a full evaluation of the safety of SiO 2 –gentamicin nanohybrids in orthopedic applications.

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Continuous release of gentamicin from gold nanocarriers

Abstract: Antimicrobial activity of gentamicin can be extended through release from gold nanocarriers after conjugation.

Cited by 31 publications

References 35 publications

Controlling release kinetics of gentamicin from silica nano-carriers

Controlling release kinetics of gentamicin from silica nano-carriers

<p>Anti-inflammatory drug-eluting implant model system to prevent wear particle-induced periprosthetic osteolysis</p>

Effects of silica–gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells

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Continuous release of gentamicin from gold nanocarriers

Abstract: Antimicrobial activity of gentamicin can be extended through release from gold nanocarriers after conjugation.

Cited by 31 publications

References 35 publications

Controlling release kinetics of gentamicin from silica nano-carriers

Controlling release kinetics of gentamicin from silica nano-carriers

<p>Anti-inflammatory drug-eluting implant model system to prevent wear particle-induced periprosthetic osteolysis</p>

Effects of silica&ndash;gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells

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Effects of silica–gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells