Continuous purification of influenza A virus particles using pseudo-affinity membrane chromatography

Fortuna, Ana Raquel; Taft, Florian; Villain, Louis; Wolff, Michael W.; Reichl, Udo

doi:10.1016/j.jbiotec.2021.10.003

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…Fortuna et al [ 120 ] used a three-column periodical counter-current device with Sartobind® SC membrane adsorber in combined elution mode continuous purification of influenza A virus A/PR/8/34 virus particles. The effective usage of binding capacity can be increased to 80%, which can be successfully used as a continuous mode for the purification of cell-derived influenza virus particles [ 120 ].…”

Section: Applicationsmentioning

confidence: 99%

Recent development and application of membrane chromatography

Chen

Cong

et al. 2022

Anal Bioanal Chem

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Membrane chromatography is mainly used for the separation and purification of proteins and biological macromolecules in the downstream processing process, also applications in sewage disposal. Membrane chromatography is recognized as an effective alternative to column chromatography because it significantly improves chromatography from affinity, hydrophobicity, and ion exchange; the development status of membrane chromatography in membrane matrix and membrane equipment is thoroughly discussed, and the applications of protein capture and intermediate purification, virus, monoclonal antibody purification, water treatment, and others are summarized. This review will provide value for the exploration and potential application of membrane chromatography. Graphical abstract

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Section: Applicationsmentioning

confidence: 99%

Recent development and application of membrane chromatography

Chen

Cong

et al. 2022

Anal Bioanal Chem

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“…Biologically active compounds, whether small organic chiral molecules or peptides, and proteins require even more robust methods in separation because a trace impurity of similar molecules could interfere with the functions and even be lethal if introduced to the human body. Hence, a special type of membrane that recognizes specific molecules, termed an affinity membrane, was developed. − One way of introducing molecular recognition was by imprinting techniques, in which template molecules were introduced covalently or noncovalently during membrane synthesis, followed by leaching of the templates, leaving specific pores in the membranes that have an affinity toward molecules resembling the templates. The aim is to achieve high permeability with maintained permselectivity.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Affinity Membrane: A Review

et al. 2022

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A molecularly imprinted affinity membrane (MIAM) can perform separation with high selectivity due to its unique molecular recognition introduced from the molecular-printing technique. In this way, a MIAM is able to separate a specific or targeted molecule from a mixture. In addition, it is possible to achieve high selectivity while maintaining membrane permeability. Various methods have been developed to produce a MIAM with high selectivity and productivity, with their respective advantages and disadvantages. In this paper, the MIAM is reviewed comprehensively, from the fundamentals of the affinity membrane to its applications. First, the development of a MIAM and various preparation methods are presented. Then, applications of MIAMs in sensor, metal ion separation, and organic compound separation are discussed. The last part of the review discusses the outlook of MIAMs for future development.

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“…These developments cannot compromise on product quality and purity, and should ideally drive the cost-effectiveness of the process. In order to meet these challenges, scientists focus on the following bioprocess and analytical Research Topic: i) In-process accelerated analytics ( Lothert et al, 2022 ), ii) process automation and data science ( Vormittag et al, 2020 ), and high-throughput technology to speed up process development ( Marchel et al, 2019 ; Hillebrandt et al, 2021 ), iii) process translation from the development scale to the production scale, iv) platform technologies in the area of USP ( Felberbaum, 2015 ; Farzaneh et al, 2017 ; Chambers et al, 2018 ; Tan et al, 2021 ) and DSP ( Marichal-Gallardo et al, 2017 ; Fortuna et al, 2018 ; Hillebrandt et al, 2020 ; Lothert et al, 2020 ), v) continuous manufacturing processes ( Krober et al, 2013 ; Fischer et al, 2018 ; Fortuna et al, 2021 ), vi) single-use technologies and closed systems, vii) modeling of production processes, and (viii) formulation strategies for nanoplexes ( Batty et al, 2021 ). Some of this research is addressed in the following Research Topic.…”

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confidence: 99%