Continuous protein recovery using a liquid–solid circulating fluidized bed ion exchange system: Modelling and experimental studies

Abstract

In this study, a continuous protein recovery process using a Liquid–Solid Circulating Fluidized Bed (LSCFB) ion exchange system is described and a model with known kinetics has been developed. Experiments and computer simulations using Matlab™ are conducted at different operating conditions. The effects of hydro‐dynamic parameters and kinetic parameters on the performance of the LSCFB ion exchange system are discussed. The model is shown to be applicable for the design of LSCFB ion exchange systems for protein recovery.

Solid holdup in liquid solid circulating fluidized bed with viscous liquid medium

“…Effective solid liquid contacting, uniform temperature, accommodating different particulate materials with high liquid throughputs, less back mixing and improved heat transfer performance have shown to be of advantage in some chemical and bio process industries [1][2][3]. LSCFB find applications in wide variety of industrial processes such as production of linear alkyl benzene, continuous protein recovery from waste whey solutions, biological nutrient removal in municipal waste water and in the removal and recovery of cesium from liquid radioactive nuclear streams [4][5][6]. These processes generally involve a liquid phase reactant and a solid phase catalyst or adsorbent.…”

Hydrodynamics of a liquid–solid circulating fluidized bed: Effect of dynamic leak

“…The reactivated catalyst is fed back into the riser from the downcomer. Similarly, in the case of continuous recovery of proteins from un-clarified whole broth [7][8][9], the adsorption and desorption (regeneration) of proteins are carried out separately in the downcomer and the riser, respectively, in a continuous mode with ion exchange particles circulated between the two columns. For the removal of biological nutrient from waste water, solid particles with biofilm are circulated between the riser where nitrification occurs and the downcomer where de-nitrification is carried out simultaneously [10].…”

Numerical simulation of counter-current flow field in the downcomer of a liquid–solid circulating fluidized bed

“…The LSCFB ion exchange system used in this study was developed by Lan et al (2000), where an experimental study on hydrodynamics and kinetics of the protein extraction process in this LSCFB ion exchange system was conducted. In that study, a lab scale system was designed and manufactured.…”

“…The proposed model is used to examine the effect of the operating condition on the hydrodynamic characteristics and to obtain the residence time distribution (RTD) of solid particles using a pulse technique. (Lan et al, 2000).…”

“…To take advantage of the exceptional characteristics of LSCFBs, Lan, Zhu, Bassi, & Margaritis (2002) and Lan et al (2000) reported an LSCFB ion-exchange system for the continuous extraction of proteins. In addition to modeling the riser, they also developed a semi-empirical correlation to predict the solids holdup in the downcomer.…”