Continuous production of poly‐β‐hydroxybutyrate by high‐cell‐density cultivation of <i>Wautersia eutropha</i>

Khanna, Shilpi; Srivastava, Ashok K.

doi:10.1002/jctb.1868

Cited by 13 publications

(8 citation statements)

References 22 publications

(18 reference statements)

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“…In this study, comparatively higher dilution rate of 0.14 1/h was maintained in a continuous production of PHB with expensive glucose as a substrate. Using relatively similar dilution rate of 0.1 1/h and almost similar concentration of expensive pure fructose of 90 g/l, comparatively lower concentration of PHB production (5.5 g/l) was achieved by Khanna and Srivastava (2008a).…”

Section: Enhanced Batch Production Of Phb Through Fermentermentioning

confidence: 99%

“…While working with maximum concentration of sucrose, i.e., 200 g/l, the microorganism showed the ability to accumulate PHB up to 64% of its dry cell weight. To improve the overall effectiveness of the process continuous and membrane cell recycle fed-batch system also have been investigated by few researchers (Khanna and Srivastava 2008a;Koller and Muhr 2014;Ienczak et al 2016). Maintaining adequate oxygen concentration and proper sterility during cell recycle phase are the two biggest challenges which hinder the application of membrane technology in PHB production process (Cui and Muralidhara 2010; Koller and Muhr 2014).…”

Section: Enhanced Batch Production Of Phb Through Fermentermentioning

confidence: 99%

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Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Dey

Rangarajan

2017

3 Biotech

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Experimental investigations were carried out for (MTCC 1472)-based improved production of poly-3 hydroxy butyrate (PHB) through induced nitrogen limiting fed-batch cultivation strategies. Initially Plackett-Burman design and response surface methodology were implemented to optimize most influencing process parameters. With optimized process parameter values, continuous feeding strategies ware applied in a 5-l fermenter with table sugar concentration of 100 g/l, nitrogen concentration of 0.12 g/l for fed-batch fermentation with varying dilution rates of 0.02 and 0.046 1/h. To get enriched production of PHB, concentration of the sugar was further increased to 150 and 200 g/l in feeding. Maximum concentrations of PHB achieved were 22.35 and 23.07 g/l at those dilution rates when sugar concentration maintains at 200 g/l in feeding. At maximum concentration of PHB (23.07 g/l), productivity of 0.58 g/l h was achieved with maximum PHB accumulation efficiency up to 64% of the dry weight of biomass. High purity of PHB, close to medical grade was achieved after surfactant hypochlorite extraction method, and it was further confirmed by SEM, EDX, and XRD studies.

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Section: Enhanced Batch Production Of Phb Through Fermentermentioning

confidence: 99%

Section: Enhanced Batch Production Of Phb Through Fermentermentioning

confidence: 99%

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Dey

Rangarajan

2017

3 Biotech

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“…R. eutropha which is the most widely used for PHA production is reported to produce PHB, P(3HB:3HV), P(3HB:4HB) and P(3HB:3HV:4HB) from a wide range of substrates like sugars, glycerol, FAs, dairy products, agricultural-industrial waste, etc. [21][22][23].…”

Section: Bacterial Pha Synthasesmentioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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“…eutropha (today: C. necator) NRRL B14690 strain was the subject of batch/fed-batch/continuous cultivation and mathematical modeling by Khanna and Srivastava [58][59][60][61] . For the microbial growth rate, these authors applied the double substrate (fructose and nitrogen) Hill (sigmoidal) kinetic expression corrected by the double substrate inhibition term according to Luong.…”

mentioning

confidence: 99%

“…These authors used the models proposed by Mulchandani 36 , Raje and Srivastava 53 , as well as Khanna and Srivastava [58][59][60][61] as the starting point to con-struct the basic formulation of four models. They modified these models by: (i) changing the expression for the specific growth rate (μ);…”

mentioning

confidence: 99%

Mathematical Modelling as a Tool for Optimized PHA Production

Novák

Koller

Braunegg

et al. 2015

Chem.Biochem.Eng.Q.

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c ARENA -Association for resource efficient and sustainable technologies, Inffeldgasse 21b, 8010 Graz, AustriaThe potential of poly(hydroxyalkanoates) (PHAs) to replace conventional plastic materials justifies the increasing attention they have drawn both at lab-scale and in industrial biotechnology.The improvement of large-scale productivity and biochemical/genetic properties of producing strains requires mathematical modeling and process/strain optimization procedures. Current models dealing with structurally diversified PHAs, both structured and unstructured, can be divided into formal kinetic, low-structured, dynamic, metabolic (high-structured), cybernetic, neural networks and hybrid models; these attempts are summarized in this review. Characteristic properties of specific groups of models are stressed in light of their benefit to the better understanding of PHA biosynthesis, and their applicability for enhanced productivity. Unfortunately, there is no single type of mathematical model that expresses exactly all the characteristics of producing strains and/or features of industrial-scale plants; in addition, the different requirements for modelling of PHA production by pure cultures or mixed microbial consortia have to be addressed. Therefore, it is crucial to sophisticatedly adapt and fine-tune the modelling approach accordingly to actual processes, as the case arises. For "standard microbial cultivations and everyday practices", formal kinetic models (for simple cases) and "low-structured" models will be appropriate and of great benefit. They are relatively simple and of low computational demand.To overcome the specific weaknesses of different established model types, some authors use hybrid models. Here, satisfying compromises can be achieved by combining mechanistic, cybernetic, and neural and computational fluid dynamics (CFD) models. Therefore, this hybrid modelling approach appears to constitute the most promising solution to generate a holistic picture of the entire PHA production process, encompassing all the benefits of the original modelling strategies. Complex growth media require a higher degree of model structuring. For scientific purposes and advanced development of industrial equipment in the future, real systems will be modelled by highly organized hybrid models.All solutions related to modelling PHA production are discussed in this review.

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Continuous production of poly‐β‐hydroxybutyrate by high‐cell‐density cultivation of Wautersia eutropha

Cited by 13 publications

References 22 publications

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Mathematical Modelling as a Tool for Optimized PHA Production

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