Continuous production of fructooligosaccharides and invert sugar by chitosan immobilized enzymes: Comparison between in fluidized and packed bed reactors

Lorenzoni, André Soibelmann Glock; Aydos, Luiza Fichtner; Klein, Manuela Poletto; Ayub, Marco Antônio Záchia; Rodrigues, Rafael C.; Hertz, Plinho Francisco

doi:10.1016/j.molcatb.2014.11.002

Cited by 49 publications

(31 citation statements)

References 29 publications

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“…The results of this study are anticipated to contribute to the development of alternative and low-cost CG sorbent materials for the sequestration of agrochemicals in aquatic environments. The CG materials can be further modified for use in large-scale applications such as fluidized bed reactors (Lorenzoni et al 2015;Zhou et al 2014a) for the sequestration of contaminants in drinking water reservoirs.…”

Section: Introductionmentioning

confidence: 99%

Sequestration of agrochemicals from aqueous media using cross-linked chitosan-based sorbents

Mohamed

Wilson

2016

Adsorption

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The sorption properties are reported for chitosan and its cross-linked forms (chitosan-glutaraldehyde; CG) with some model agrochemical sorbates [pentachlorophenol (PCP), 2,4-dichlorophenol (2,4-DCP) and 2,4-dichlorophenoxy acetic acid (2,4-D), dicamba and carbofuran]. The CG cross-linked materials were prepared at variable C:G monomer mole ratios: 1:0.5 (CG1), 1:1 (CG2), (CG3). The sorbents were characterized using diffuse reflectance infrared Fourier transform spectroscopy, thermogravimetric analysis and a dye sorption method using phenolphthalein. The sorption studies were carried out in aqueous solution at pH 9 except for dicamba and carbofuran (pH 7). The isotherm results were evaluated by the Sips, Freundlich, and Langmuir models. The Sips model provided the ''best-fit'' results where the sorption capacity increased as the cross-linker content of the CG materials increased. The relative uptake for chitosan and its cross-linked forms adopted the following order: PCP [ 2,4-DCP [ 2,4-D. In the case of dicamba and carbofuran, the former had a higher sorptive uptake. The variable uptake of the sorbates were attributed to their relative lipophilicity where the main driving force of these solid-solution systems relates to hydrophobic effects, in accordance with the tunable physicochemical properties of the chitosan sorbent materials.

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Section: Introductionmentioning

confidence: 99%

Sequestration of agrochemicals from aqueous media using cross-linked chitosan-based sorbents

Mohamed

Wilson

2016

Adsorption

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“…For each resin, the adsorption was carried out at two temperatures (25 and 62°C). The two temperatures were selected to minimize any form of protein inactivation or interactions due to many temperature variations as the enzyme preparation is not completely purified . A summary of the immobilization results is displayed in Table .…”

Section: Resultsmentioning

confidence: 99%

Amberlite IRA 900 versus calcium alginate in immobilization of a novel, engineered β‐fructofuranosidase for short‐chain fructooligosaccharide synthesis from sucrose

Bedzo

Trollope

Gottumukkala

et al. 2019

Biotechnology Progress

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The immobilization of β-fructofuranosidase for short-chain fructooligosaccharide (scFOS) synthesis holds the potential for a more efficient use of the biocatalyst. However, the choice of carrier and immobilization technique is a key to achieving that efficiency. In this study, calcium alginate (CA), Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) were tested as supports for immobilizing a novel engineered β-fructofuranosidase from Aspergillus japonicus for scFOS synthesis. Several immobilization parameters were estimated to ascertain the effectiveness of the carriers in immobilizing the enzyme. The performance of the immobilized biocatalysts are compared in terms of the yield of scFOS produced and reusability. The selection of carriers and reagents was motivated by the need to ensure safety of application in the production of food-grade products. The CA and AI900 both recorded impressive immobilization yields of 82 and 62%, respectively, while the DMM recorded 47%. Enzyme immobilizations on CA, AI900 and DMM showed activity recoveries of 23, 27, and 17%, respectively. The CA, AI900 immobilized and the free enzymes recorded their highest scFOS yields of 59, 53, and 61%, respectively. The AI900 immobilized enzyme produced a consistent scFOS yield and composition for 12 batch cycles but for the CA immobilized enzyme, only 6 batch cycles gave a consistent scFOS yield. In its first record of application in scFOS production, the AI900 anion exchange resin exhibited potential as an adequate carrier for industrial application with possible savings on cost of immobilization and reduced technical difficulty. K E Y W O R D S adsorption, entrapment, fructooligosaccharides, frustosyltransferase, immobilization, ion exchange

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“…Abdel‐Naby et al also reported better performance of FBR for CGTase production compared with shaking flask and packed bed reactor. Well mixing and efficient solid–liquid interaction in the FBRs, which provided favorable heat and mass transfer rates led to promotion of metabolite formation while column clogging, compression of solid particles, and liquid channelization were reported as the weaknesses of packed bed reactors .

% c o n v e r s i o n = \frac{S_{0} - S_{normalf}}{S_{0}} \times 100

…”

Section: Resultsmentioning

confidence: 99%

Bacillussp. DSM 2523 entrapped within chitosan beads used as the whole cell biocatalyst for the production of cyclodextrin glucanotransferase in a fluidized bed bioreactor

et al. 2016

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The production of cyclodextrin glucanotransferase (CGTase) from starch with the use of Bacillus sp. DSM 2523 cells entrapped within chitosan beads was studied in a constructed fluidized bed reactor. The experiments were monitored in terms of b-cyclodextrin (b-CD) formation and starch utilization. The central composite design concept was applied to statistically describe the effects of biocatalyst loading and initial starch concentration. The b-CD production and starch utilization were expressed according to quadratic and two-factor interaction models, respectively. The hydrodynamics of the reactor were characterized using chitosan beads of various sizes. The dependence of the drag coefficient on the terminal settling velocity (U t,s ) and terminal Reynolds number was determined. The minimum fluidization velocity was estimated from the Ergun equation based on the relationship between the Reynolds number at minimum fluidization and the Archimedes number. The exponential expression of Richardson-Zaki was applicable in describing the influence of the ratio of the superficial liquid velocity to U t,s on the bed voidage.

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Continuous production of fructooligosaccharides and invert sugar by chitosan immobilized enzymes: Comparison between in fluidized and packed bed reactors

Cited by 49 publications

References 29 publications

Sequestration of agrochemicals from aqueous media using cross-linked chitosan-based sorbents

Sequestration of agrochemicals from aqueous media using cross-linked chitosan-based sorbents

Amberlite IRA 900 versus calcium alginate in immobilization of a novel, engineered β‐fructofuranosidase for short‐chain fructooligosaccharide synthesis from sucrose

Bacillussp. DSM 2523 entrapped within chitosan beads used as the whole cell biocatalyst for the production of cyclodextrin glucanotransferase in a fluidized bed bioreactor

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