Continuous production of d-lactic acid from cellobiose in cell recycle fermentation using β-glucosidase-displaying Escherichia coli

Aso, Yoichi; Tsubaki, Mikikazu; Long, Bui Hoang Dang; Murakami, Ryo; Nagata, Kenichi; Okano, Hirohisa; Dung, Ngo Thi Phuong; Ohara, Hirotaka

doi:10.1016/j.jbiosc.2018.09.011

Cited by 25 publications

(13 citation statements)

References 37 publications

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“…Continuous modes include chemostat, cell cycle, and cell immobilization, among others. Aso et al used cell cycle fermentation to improve the productivity of lactic acid by 4-fold that of batch fermentation [ 33 ]. Cell recycling increased the productivity of butanol production by 6-fold that of high cell density fermentation [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Enhancing the efficiency of L-tyrosine by repeated batch fermentation

Chen

et al. 2020

Bioengineered

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L-tyrosine is a widely used aromatic amino acid with an increasing market demand. Improving the parameters of L-tyrosine production results in a more cost-effective process that is of great interest for industrial applications. E. coli GHLTYR-168 was used to ferment L-tyrosine, with a productivity of 1.73 g/(L•h) and a yield of 17.6%. To further increase its production efficiency, repeated batch fermentation was applied to L-tyrosine, in which both replacement time points and ratios were studied during different fermentations. The broth substitution time point had no significant effect on L-tyrosine subjected to repeated batch fermentation, and 70% broth replacement ratio was the best choice. Repeated batch fermentation was performed in 5 batches within 100 h, among which the efficiency of the third batch fermentation was the highest. In the third batch fermentation, the productivity and yield were 2.53 g/(L•h) and 30.1%, respectively. Compared with that during fedbatch fermentation, the productivity and yield of L-tyrosine increased by 43.8% and 74.0%, respectively, during repeated batch fermentation. This is the highest level of L-tyrosine fermentation reported so far. Thus, repeated batch fermentation of L-tyrosine can improve its production efficiency.

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Section: Resultsmentioning

confidence: 99%

Enhancing the efficiency of L-tyrosine by repeated batch fermentation

Chen

et al. 2020

Bioengineered

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“…Na-2 118 0.97 4.37 Glucose [ 39 ] B. sp. P38 180 0.96 2.4 Cellulosic hydrolysate [ 37 ] E. coli Engineered E. coli 60–62.2 0.80–0.90 Glucose [ 348 ] Engineered E. coli 45.5–51.8 0.91–0.99 Glucose [ 52 ] Engineered E. coli 40 0.93 Xylose [ 57 ] Engineered E. coli 56.8 0.88 0.94 Glycerol [ 350 ] E. coli AC-521 85 0.85 1.0 Sucrose [ 54 ] E. coli K12 strain 32 0.85 0.44 Glycerol [ 59 ] E. coli 75 0.85 1.18 Molasses [ 351 ] lactogenic Escherichia coli strain JU15 40 0.6 Corn stover [ 352 ] E. coli BW25113 (DpflA) (engineered) 5.2 22.5 0.06 cellobiose [ 353 ] <...>…”

Section: La Producing Microorganismsmentioning

confidence: 99%

Lactic acid production – producing microorganisms and substrates sources-state of art

Abedi

Hashemi

2020

Heliyon

224

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Lactic acid is an organic compound produced via fermentation by different microorganisms that are able to use different carbohydrate sources. Lactic acid bacteria are the main bacteria used to produce lactic acid and among these, Lactobacillus spp. have been showing interesting fermentation capacities. The use of Bacillus spp. revealed good possibilities to reduce the fermentative costs. Interestingly, lactic acid high productivity was achieved by Corynebacterium glutamicum and E. coli, mainly after engineering genetic modification. Fungi, like Rhizopus spp. can metabolize different renewable carbon resources, with advantageously amylolytic properties to produce lactic acid. Additionally, yeasts can tolerate environmental restrictions (for example acidic conditions), being the wildtype low lactic acid producers that have been improved by genetic manipulation. Microalgae and cyanobacteria, as photosynthetic microorganisms can be an alternative lactic acid producer without carbohydrate feed costs. For lactic acid production, it is necessary to have substrates in the fermentation medium. Different carbohydrate sources can be used, from plant waste as molasses, starchy, lignocellulosic materials as agricultural and forestry residues. Dairy waste also can be used by the addition of supplementary components with a nitrogen source.

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“…Biomass quantification (X) was carried out by OD at 600 nm (OD600) with a Genesys 10S UV–vis spectrophotometer (Thermo Scientific, U.S.A) in which, OD600 measured values were correlated with the weight of dry cells (g DCW/L) using a calibration curve [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Kinetic analysis and modeling of L-valine production in fermentation batch from E. coli using glucose, lactose and whey as carbon sources

Carranza-Saavedra

Henao

Montoya

2021

Biotechnology Reports

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Highlights First test in which whey and E. coli are used to obtain L-valine. Kinetic properties and kinetic models of L-valine production were studied. Highest L-valine production was obtained when whey was used as substrate. Highest specific growth rate was achieved when using deproteinized whey.

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Continuous production of d-lactic acid from cellobiose in cell recycle fermentation using β-glucosidase-displaying Escherichia coli

Cited by 25 publications

References 37 publications

Enhancing the efficiency of L-tyrosine by repeated batch fermentation

Enhancing the efficiency of L-tyrosine by repeated batch fermentation

Lactic acid production – producing microorganisms and substrates sources-state of art

Kinetic analysis and modeling of L-valine production in fermentation batch from E. coli using glucose, lactose and whey as carbon sources

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