Continuous phenol biodegradation in a simple packed bed bioreactor of calcium alginate-immobilized Candida tropicalis (NCIM 3556)

Varma, Rita; Gaikwad, Bhaskar G.

doi:10.1007/s11274-009-0236-7

Cited by 12 publications

(4 citation statements)

References 17 publications

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“…Washed cells harvested as specified above, were immobilized by entrapment in a sodium alginate matrix (Varma and Gaikwad 2010 ; Givry et al 2008 ). For the entrapment, 200 mg (dry weight basis) of cells was resuspended in 10 mL of Tris–HCl buffer (100 mM, pH 7).…”

Section: Methodsmentioning

confidence: 99%

Stereo-selective conversion of mandelonitrile to (R)-(−)-mandelic acid using immobilized cells of recombinant Escherichia coli

et al. 2012

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Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2-hydroxy-2-phenylacetonitrile) to ( R )-(−)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hydrolysis reaction were optimized in shake flasks and in a packed bed reactor. In shake flasks the best conditions for the reaction were a temperature of 40 °C, pH 8, biocatalyst bead diameter of 4.3 mm, sodium alginate concentration in the gel matrix of 2 % (w/v, g/100 mL), a cell dry mass concentration in the bead matrix of 20 mg/mL, an initial substrate concentration of 50 mM and a reaction time of 60 min. Under these conditions, the conversion of mandelonitrile was nearly 95 %. In the packed bed reactor, a feed flow rate of 20 mL/h at a substrate concentration of 200 mM proved to be the best at 40 °C, pH 8, using 4.3 mm beads (2 % w/v sodium alginate in the gel matrix, 20 mg dry cell concentration per mL of gel matrix). This feed flow rate corresponded to a residence time of 0.975 h in the packed bed.

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Section: Methodsmentioning

confidence: 99%

Stereo-selective conversion of mandelonitrile to (R)-(−)-mandelic acid using immobilized cells of recombinant Escherichia coli

et al. 2012

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“…Phenolic compounds and their derivatives are considered as serious pollutants because even at low concentrations they are toxic, carcinogenic, mutagenic and teratogenic. If released into the environment, they may accumulate in ground water, surface water, or soil, thus representing an issue of great environmental concern (Coniglio et al 2008;Gianfreda et al 2003;Tang et al 2008;Varma and Gaikwad 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of dirhamnolipid on the removal of phenol catalyzed by laccase in aqueous solution

Liu

Zeng

Zhong

et al. 2011

World J Microbiol Biotechnol

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In this study, the effects of three surfactants, i.e. the anionic biosurfactant dirhamnolipid (diRL), the cationic surfactant hexadecyltrimethyl ammonium bromide (CTAB), and the anionic surfactant sodium dodecyl sulfate (SDS), on the removal of phenol catalyzed by laccase were studied first. CTAB and SDS were detrimental, while diRL improved phenol removal and was selected for detailed research. The biosurfactant increased the activity of laccase and the removal of phenol with the increase of diRL concentrations from 10.6 to 318 μM. DiRL at 318 μM improved the removal when the initial concentrations of phenol were from 50 to 400 mg/l. In particular, the removal of phenol with 318 μM diRL was 4.3-6.4 folds that of the controls within 24 h when the initial concentration of phenol was 400 mg/l. The presence of diRL at 318 μM also caused the complete removal (above 98%) of phenol at concentrations from 50 to 400 mg/l after 24 h. The enhancement of phenol removal was over a wide range of pH and temperatures, and the highest removal efficiency was obtained at pH 6.0 and 50°C. The results suggest that diRL had potential application in the enhancement of phenols removal catalyzed by laccase in water treatment or remediation.

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“…Thus, immobilization of microbial cells has many advantages such as the reuse of biomass and phenol is confirmed as a cheap method for oxidizing the effluents [14]. Unique characteristics of Polyurethane deserve attractiveness as Polyurethane offers rubber elasticity, and resistance to fats, oil and solvents.…”

Section: Introductionmentioning

confidence: 99%

Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization

Al-Defiery,

Reddy

2024

J.port.sci.res.

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One of the primary concerns of the environment is the increment of the xenobiotics levels, which are released in the natural ecosystem. Phenol has been documented as a pollutant because it has a significant role in water contamination; this will, therefore have an impact on the health of humans. Phenol degradation studies were carried out using a mineral salts medium containing various percentages (v/v) of Ca-alginate beads, polyurethane foam, agar-agar and agarose in batches of culture for 1.5 g/L phenol degradation by immobilized cells of Rhodococcus pyridinivorans GM3 during 24 hours of incubation at 32ºC, 200 rpm and pH 8.5. The results showed that a typical concentration of 3% (w/v) of the sodium alginate to form synthetic Ca-alginate beads was supporting phenol degradation which also emphasizes the structural stability of Ca-alginate beads. The concentration of 1.5 g/L phenol was completely degraded observed within 24 hours at 8% of the Ca-alginate beads immobilized cell and 10% of size cubes 0.125 cm3 of the polyurethane foam immobilized cell. Whilst, the degradation of 1.5 g/L of phenol concentration within 24 hours on both agar and agarose was 16% and 24% at cubes of size 0.125 cm3 and 1.0 cm3 respectively. However, the study of immobilization showed that Ca-alginate immobilized R. pyridinivorans GM3 was more efficient than polyurethane foam, agar and agarose.

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Continuous phenol biodegradation in a simple packed bed bioreactor of calcium alginate-immobilized Candida tropicalis (NCIM 3556)

Cited by 12 publications

References 17 publications

Stereo-selective conversion of mandelonitrile to (R)-(−)-mandelic acid using immobilized cells of recombinant Escherichia coli

Stereo-selective conversion of mandelonitrile to (R)-(−)-mandelic acid using immobilized cells of recombinant Escherichia coli

Effect of dirhamnolipid on the removal of phenol catalyzed by laccase in aqueous solution

Degradation of phenol by Rhodococcus pyridinivorans GM3 immobilization

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