Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays

Hung, Paul J.; Lee, Philip J.; Sabounchi, Poorya; Lin, Robert; Lee, Luke P.

doi:10.1002/bit.20289

Cited by 474 publications

(341 citation statements)

References 22 publications

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“…[17][18][19] We developed here a perfusion system for the cultivation of free floating cells by incorporating pneumatic microvalves. Two pieces of the patterned PDMS for fluidic channels/chambers and pneumatic valves were overlayered with precise alignment to prepare a PDMS perfusion chip with a 2 Â 4 array of chambers and mounted on a glass slide coated with PDMS adhesive layer.…”

Section: A Chip Design and Operationmentioning

confidence: 99%

A microfluidic perfusion platform for cultivation and screening study of motile microalgal cells

Park

Kim

et al. 2014

Biomicrofluidics

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Systematic screening of algal cells is getting huge interest due to their capability of producing lipid-based biodiesel. Here, we introduce a new microfluidic platform composed of an array of perfusion chambers designed for long-term cultivation and preliminary screening of motile microalgal cells through loading and releasing of cells to and from the chambers. The chemical environment in each perfusion chamber was independently controlled for 5 days. The effect of nitrogen-depletion on the lipid production, phototaxis behavior in the absence of Ca 2þ , and cytotoxic effect of herbicide on microalgal cells was successfully monitored and compared with simultaneous control experiments on the platform. The present methodology could be extended to effective screening of algal cells and various cell lines for the production of biodiesel and other useful chemicals. V C 2014 AIP Publishing LLC.

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Section: A Chip Design and Operationmentioning

confidence: 99%

A microfluidic perfusion platform for cultivation and screening study of motile microalgal cells

Park

Kim

et al. 2014

Biomicrofluidics

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“…[1][2][3][4] For example, in islet transplantation for the treatment of type 1 diabetes, a properly engineered coating layer may protect transplanted cells against host-immune system rejection, while avoiding the chronic use of immunosuppression regimens. 5 This biocompatible coating layer may protect against the immune response by blocking immune cells and antibodies, while the semi-permeable nature of the coating membrane allows small molecules like oxygen and insulin to diffuse in and out.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic conformal coating of non-spherical magnetic particles

et al. 2014

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We present the conformal coating of non-spherical magnetic particles in a colaminar flow microfluidic system. Whereas in the previous reports spherical particles had been coated with thin films that formed spheres around the particles; in this article, we show the coating of non-spherical particles with coating layers that are approximately uniform in thickness. The novelty of our work is that while liquid-liquid interfacial tension tends to minimize the surface area of interfacesfor example, to form spherical droplets that encapsulate spherical particles-in our experiments, the thin film that coats non-spherical particles has a non-minimal interfacial area. We first make bullet-shaped magnetic microparticles using a stopflow lithography method that was previously demonstrated. We then suspend the bullet-shaped microparticles in an aqueous solution and flow the particle suspension with a co-flow of a non-aqueous mixture. A magnetic field gradient from a permanent magnet pulls the microparticles in the transverse direction to the fluid flow, until the particles reach the interface between the immiscible fluids. We observe that upon crossing the oil-water interface, the microparticles become coated by a thin film of the aqueous fluid. When we increase the two-fluid interfacial tension by reducing surfactant concentration, we observe that the particles become trapped at the interface, and we use this observation to extract an approximate magnetic susceptibility of the manufactured non-spherical microparticles. Finally, using fluorescence imaging, we confirm the uniformity of the thin film coating along the entire curved surface of the bullet-shaped particles. To the best of our knowledge, this is the first demonstration of conformal coating of non-spherical particles using microfluidics. V C 2014 AIP Publishing LLC. [http://dx

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“…Simplifying their fabrication permitted exploiting the many fascinating properties present in microfluidic systems, such as the ones shown in Figure 1, which include: 1) their capability to manipulate fluids at microscales; 2) the effective mass and heat transfer that develops due to their high surface to volume ratios; and 3) the dominance of viscous forces within these systems, which facilitate the creation of laminar flows (Gravesen et al, 1993) and allow the generation of diffusive chemical gradients when two substances are transported in parallel within the same channel. (Takayama et al, 2001;Hung et al, 2004;Wu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Environmentally responsive polymeric "intelligent" materials: the ideal components of non-mechanical valves that control flow in microfluidic systems

Morones‐Ramírez¹

2010

Braz. J. Chem. Eng.

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-Miniaturization and commercialization of integrated microfluidic systems has had great success with the development of a wide variety of techniques in microfabrication, since they allowed their construction at a low cost and by following simple step-series procedures. However, one of the major challenges in the design of microfluidic systems is to achieve control of flow and delivery of different chemical reagents. This feature is especially important when using microfluidic systems in the development of cell culture systems, the construction of labs on a chip and the fabrication and design of chemical microreactors. Spatiotemporal control of the microenvironment in microfluidic devices has been only partially achieved by incorporating actuator parts (mechanical and non-mechanical) within these devices; nevertheless, recently there has been enormous progress due to advances in the materials sciences, and the development of novel polymeric "intelligent" materials. These materials have proved to be excellent candidates in the construction of non-mechanical actuators in the form of environmentally responsive valves. These valves can more efficiently control flows because these "intelligent" materials are capable of undergoing conformational changes and phase transitions in response to different local or external environmental stimuli; allowing them to turn the valves from "on" to "off". In addition, these valves have very simple designs, and are easy and cheap to incorporate into microfluidic systems. Therefore, although there are many reviews that focus on the development and design of non-mechanical actuators, the following review proceeds to describe the exciting characteristics, potential uses and synthesis methods of the building blocks of the most recent and innovative non-mechanical valves, environmentally responsive polymeric "intelligent" materials. In addition, the last section of this review will focus on the synthesis of composite materials that are capable of responding to more than one type of stimulus, since these materials are believed to be the future components that will boost the development of microfluidic systems with spatiotemporal controlled microenvironments.

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Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays

Abstract: We present for the first time a microfluidic cell culture array for long-term cellular monitoring. The 10 Â

Cited by 474 publications

References 22 publications

A microfluidic perfusion platform for cultivation and screening study of motile microalgal cells

A microfluidic perfusion platform for cultivation and screening study of motile microalgal cells

Microfluidic conformal coating of non-spherical magnetic particles

Environmentally responsive polymeric "intelligent" materials: the ideal components of non-mechanical valves that control flow in microfluidic systems

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