Continuous measurement of tissue blood flow by laser-Doppler spectroscopy

Stern, Michael D.; Lappe, D. L.; Bowen, Peter; Chimosky, J. E.; Holloway, Greg; Keiser, H. R.; Bowman, Robert L.

doi:10.1152/ajpheart.1977.232.4.h441

Cited by 242 publications

(175 citation statements)

References 11 publications

(1 reference statement)

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“…In the experiments reported, muscle perfusion was measured with a probe sensitive to movement of red blood cells to a depth of Ϸ1 mm in the regional microcirculation. 31 Unexpectedly, the application of US improved tissue perfusion, and this resulted in reversal of acidosis. Although the laser-Doppler measurement is limited to 1 mm in depth, the prolonged duration of the US effect accompanied by normalization of tissue pH and muscle color suggests that it was more general.…”

Section: Suchkova Et Al Ultrasound Increases Thrombolysis and Perfusionmentioning

confidence: 99%

Effect of 40-kHz Ultrasound on Acute Thrombotic Ischemia in a Rabbit Femoral Artery Thrombosis Model

2000

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Background —We have shown previously that 40-kHz ultrasound (US) at low intensity accelerates fibrinolysis in vitro with little heating and good tissue penetration. These studies have now been extended to examine the effects of 40-kHz US on thrombolysis and tissue perfusion in a rabbit model. Methods and Results —Treatment was administered with either US alone at 0.75 W/cm 2 , streptokinase alone, or the combination of US and streptokinase. US or streptokinase resulted in minimal thrombolysis, but reperfusion was nearly complete with the combination after 120 minutes. US also reversed the ischemia in nonperfused muscle in the absence of arterial flow. Tissue perfusion decreased after thrombosis from 13.7±0.2 to 6.6±0.8 U and then declined further to 4.5±0.4 U after 240 minutes. US improved perfusion to 10.6±0.5 and 12.1±0.5 U after 30 and 60 minutes, respectively. This effect was reversible and declined to pretreatment values after US was discontinued. Similarly, tissue pH declined from normal to 7.05±0.02 after thrombosis, but US improved pH to 7.34±0.03 after 60 minutes. US-induced improvement in tissue perfusion and pH also occurred after femoral artery ligation, indicating that thrombolysis did not cause these effects. Conclusions —40-kHz US at low intensity markedly accelerates fibrinolysis and also improves tissue perfusion and reverses acidosis, effects that would be beneficial in treatment of acute thrombosis.

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Section: Suchkova Et Al Ultrasound Increases Thrombolysis and Perfusionmentioning

confidence: 99%

Effect of 40-kHz Ultrasound on Acute Thrombotic Ischemia in a Rabbit Femoral Artery Thrombosis Model

2000

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“…give absolute values, but shows relative changes in blood flow (for technical details and evaluation of the LDF method see Stern et al 1977). Blood flow was recorded from the common carotid artery (CCA) by means of an electromagnetic blood flow transducer fitted to an electromagnetic blood flowmeter (EMF; model AT-610G, Nihon Koden) as described before (Izumi et al 1997).…”

Section: Animalsmentioning

confidence: 99%

Sympathetic attenuation of parasympathetic vasodilatation in oro‐facial areas in the cat

Izumi

Ito

1998

The Journal of Physiology

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The present study was designed to examine the interaction between sympathetic and parasympathetic influences on blood flow in oro‐facial areas such as lower lip, palate and submandibular gland (SMG) and in the common carotid artery (CCA) in anaesthetized cats. Section of the ipsilateral superior cervical sympathetic trunk (CST) increased the basal CCA blood flow significantly. The control level with the nerve intact was comparable with that seen at 0.5‐1 Hz CST stimulation, suggesting a spontaneous discharge of around 0.5‐1 Hz in the CST fibres innervating the beds supplied by the CCA. The basal blood flow at all sites examined was reduced by CST stimulation in a frequency‐dependent manner. Electrical stimulation of the central end of the lingual nerve (LN) evoked blood flow increases in the lower lip and palate. These blood flow increases were markedly reduced by concurrent CST stimulation in a manner that was frequency dependent, but not simply related to the vasoconstrictor effect of CST stimulation. This effect of CST stimulation was not observed in tongue or SMG, even though CST stimulation evoked vasoconstriction in these tissues. A significant reduction in the level of CCA blood flow attained during LN stimulation was observed on repetitive CST stimulation only at 10 Hz, indicating that this response behaved in a fashion different from that seen in the lower lip, palate, tongue and SMG. The present study suggests that concurrent repetitive CST stimulation reduces parasympathetically mediated blood flow increases in certain oro‐facial areas (such as the lower lip and palate), but not in the tongue and SMG. This inhibitory action was not a simple additive effect (between vasoconstriction and vasodilatation) and it disappeared rapidly after the cessation of CST stimulation.

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“…In guinea pigs, Perlman et al (1959) made microscopic observation of blood flow through individual vessels of stria vascularis and described a decrease in CBF following vascular compression. Development of LD technique and its successful use in other tissues enabled researchers to measure CBF with accuracy and minimal invasiveness (Stern et al, 1977). LD rapidly became popular for measuring CBF and other physiological parameters in animal models such as guinea pig, rat, gerbil, and rabbit (Miller et al, 1983;Scheibe et al, 1990;Asami et al, 1995;Ren et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous measurement of electrocochleography and cochlear blood flow during cochlear hypoxia in rabbits

Yavuz¹,

Morawski²,

Telischi³

et al. 2005

Journal of Neuroscience Methods

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In this study, a new monitoring system is developed to measure cochlear blood flow (CBF) and electrocochleography (ECochG) during transient ischemic episodes of the cochlea. A newly designed otic probe was used for the simultaneous recordings of laser-Doppler CBF and ECochG directly from the round window (RW). The probe enabled the recording of high amplitude compound action potentials (CAP) and cochlear microphonics (CM) with few averages. Experiments were conducted on rabbits to generate episodes of cochlear ischemia by using timed compressions of the internal auditory artery (IAA). The computer monitoring system extracted and measured CAP and CM components from ECochG in real-time. Results indicate that CM and CAP generally followed CBF during compressions and releases of IAA. Both CBF values and CAP amplitudes showed an overshoot following the reperfusion. CAP amplitude measures were found to be very sensitive to ischemia showing very rapid amplitude, latency and morphological changes. CM amplitude decreased more slowly than the CAP and CBF. Simultaneous recordings of CBF and ECochG using the otic probe provide a valuable neuromonitoring tool to investigate the dynamic behavior of the cochlea during ischemia.

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Continuous measurement of tissue blood flow by laser-Doppler spectroscopy

Cited by 242 publications

References 11 publications

Effect of 40-kHz Ultrasound on Acute Thrombotic Ischemia in a Rabbit Femoral Artery Thrombosis Model

Effect of 40-kHz Ultrasound on Acute Thrombotic Ischemia in a Rabbit Femoral Artery Thrombosis Model

Sympathetic attenuation of parasympathetic vasodilatation in oro‐facial areas in the cat

Simultaneous measurement of electrocochleography and cochlear blood flow during cochlear hypoxia in rabbits

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