Continuous Measurement of Porcine Renal Cortex Microcirculation with Enhanced Thermal Diffusion Technology

Kraus, T.; Klar, E.; Osswald, Brigitte; Fernandes, L.; Mehrabi, Arianeb; Gebhard, Martha Maria; Herfarth, Ch.

doi:10.1006/jsre.1996.0159

Cited by 21 publications

(16 citation statements)

References 3 publications

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“…Previous methods, however, have been inefficient to measure local tissue perfusion (10,11). In this study, we used TDM to measure LMP distal to the LAD, which validated many other organs (11)(12)(13)(14).…”

Section: Discussionmentioning

confidence: 87%

Nicardipine Augments Local Myocardial Perfusion after Coronary Artery Reperfusion in Dogs

Jang

Kim

2003

J Korean Med Sci

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INTRODUCTIONThe measurement of perfusion is very important to understand the physiology at the tissue level. And local myocardial perfusion (LMP) can identify hypoperfused myocardial tissue more precisely. In particular, during ischemia the myocardial layers of the left ventricle can be ischemic, while the epicardial blood flow is preserved. Many drugs have been used to improve the myocardial function in patients with coronary disease, and they may affect the LMP by various mechanisms (1-5). Nicardipine, a dihydropyridine calcium antagonist, is one of the most commonly used drugs to control the high blood pressure after coronary artery bypass graft. Nicardipine has relatively selective coronary vascular action (6). However, there has been no study of identifying the nicaridipine effects in myocardium by using LMP after the coronary reperfusion.Therefore, this study was undertaken to evaluate the nicadipine's effect in myocardial layers after the coronary reperfusion. The LMP and left anterior descending coronary blood flow (BF-LAD) before and after left anterior descending coronary artery (LAD) occlusion were evaluated according to the nicardipine administration. MATERIALS AND METHODSThis investigations were performed under a protocol approved by the Institutional Animal Investigation Committee, Keimyung University, Daegu, Korea. A total of 14 mongrel dogs, weighing 20-25 kg, were entered into the study. We divided the dogs into two groups, control group (n=7) and nicardipine group (n=7). The anesthesia was induced by 20 mg/kg thiopental sodium and 0.2 mg/kg vecuronium bromide followed by tracheal intubation after 2 min. Mechanical ventilation was done with 50% nitrous oxide in oxygen and 1-1.5 vol% isoflurane with a tidal volume of 15 mL/kg with a respiratory rate of 12/min.A left thoracotomy was performed on the 5th or 6th intercostal space and LAD was exposed for occlusion and release. A thermal diffusion microprobe (TDM) was implanted in the myocardium perfused by the exposed LAD. To prevent the TDM displacement during cardiac contraction, the TDM was sutured in place. The TDM was then connected to QFlow J Korean Med Sci 2003; 18: 23-6 ISSN 1011-8934 Copyright � The Korean Academy of Medical Sciences 23 Nicardipine Augments Local Myocardial Perfusion after Coronary Artery Reperfusion in DogsNicardipine is a potent coronary and systemic vasodilator without depression of ventricular function. We investigated the changes in local myocardial perfusion (LMP) according to the nicardipine administration after coronary reperfusion in a beating canine model. A Doppler probe was placed around the left anterior descending coronary artery (LAD) and thermal diffusion microprobe was implanted in the myocardium perfused by the exposed LAD. To define the nicardipine effects, we compared the two groups (control group, n=7 vs nicardipine group, n=7). In nicardipine group, 5 g/kg/min nicardipine was infused continuously. After the release of the LAD occlusion, LAD blood flow were increased compared to the baseline of both...

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Section: Discussionmentioning

confidence: 87%

Nicardipine Augments Local Myocardial Perfusion after Coronary Artery Reperfusion in Dogs

Jang

Kim

2003

J Korean Med Sci

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“…With time, the LDF signal increased in a stepwise fashion once manipulation of the renal vessels had ceased. In this context LDF seems to be similar to other methods such as thermal diffusion technology [4].…”

Section: Discussionmentioning

confidence: 98%

“…Renal blood flow (RBF) in the immediate period following transplantation is an important indicator of viability: RBF has been shown to correlate well with the duration of cold ischaemia and the delayed onset of graft function including the need for post-transplant dialysis [2]. Since the inner medullary blood flow is low, representing only a few percent of the total RBF [3], it is not surprising to find that the renal cortical perfusion (RCP) correlates well with total RBF even in conditions of reduced total RBF [4].…”

Section: Introductionmentioning

confidence: 99%

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Intra- and Post-Operative Assessment of Renal Cortical Perfusion by Laser Doppler Flowmetry in Renal Transplantation in the Rat

Hammad¹,

Davis²,

Zhang³

et al. 2000

Eur Surg Res

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Renal blood flow (RBF) in the period immediately following transplantation has an important prognostic value. Here we report for the first time on the use of laser Doppler flowmetry (LDF) for the measurement of renal cortical perfusion (RCP) during all the important steps in renal transplantation. Left orthotopic kidney transplantation was performed in Lewis rats (n = 14) after 2 h of cold ischaemia and preservation in EuroCollins solution. Under baseline conditions, RCP in the donor and recipient kidneys were similar with a coefficient of variability of 11 and 12%, respectively. There was a progressive increase in RCP during the first 60 min after transplantation with a return to normal values 2 weeks later. In conclusion, LDF provides a rapid and continuous measure of RCP without interference to the operative site and may prove a useful tool for the measurement of RBF during kidney transplantation.

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“…Measurement of the local tissue temperature conductivity ascertained adequate positioning of TD probes in the renal cortex. Details of TD technology, validation and principles of probe placement have been already described [8].…”

Section: Experimental Designmentioning

confidence: 99%

Different impact of normo- and hypotensive brain death on renal macro- and microperfusion--an experimental evaluation in a porcine model

Mehrabi¹,

Golling²,

Körting³

et al. 2004

Nephrology Dialysis Transplantation

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Background. Despite the growing use of kidneys from living donors, organs harvested from brain dead donors are the dominant graft types used in renal transplantation. It is accepted that brain death (BD) has a damaging effect on the renal allograft, with a lower graft survival. Amongst various causes, changes in renal microperfusion could be responsible. Renocortical microperfusion was assessed during BD using thermal diffusion in a porcine model. Methods. Two types of BD were induced in two groups of pigs [hypotension (Hypo-BD): n ¼ 11; normotension (Normo-BD): n ¼ 10] and compared to controls (n ¼ 5) over a period of 210 min. We analysed systemic parameters [heart rate (HR), mean arterial blood pressure (MAP)], aortic blood flow (ABF) and renal perfusion [renal artery blood flow (RABF) and renocortical blood flow (RCBF)]. Results. Following the two distinct forms of BD induction, a stable normo-or hypotension was observed. Haemodynamic parameters were only slightly changed (control group: MAP, 62±2 mmHg; HR, 95±3/min; Normo-BD: MAP, 56±4 mmHg; HR, 104±8/min; Hypo-BD: MAP, 43±3 mmHg; HR, 112±7/min). Solely dependent on systemic haemodynamics, RABF and RCBF decreased in the Hypo-BD (RABF: 142±19 to 94±9 ml/100 g/min; RCBF: 80±4 to 52±2 ml/ 100 g/min), while in Normo-BD group RABF mildly changed (158±13 ml/100 g/min) and RCBF decreased slightly from 76±3 to 70±6 ml/100 g/min. As opposed to the Normo-BD group, animals with Hypo-BD showed a significant decrease in RABF (reduction of 34%) and RCBF (reduction of 35%) with a sharp drop of MAP (reduction of 25%), however ABF remained relatively constant.Conclusions. In this model, a reduction of renocortical microperfusion in brain dead pigs was only found during haemodynamic instability (hypotension) and could not be attributed to BD as such. Our findings would support intensive cardiocirculatory stabilization for potential BD donors in order to minimize kidney preservation damage.

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Continuous Measurement of Porcine Renal Cortex Microcirculation with Enhanced Thermal Diffusion Technology

Cited by 21 publications

References 3 publications

Nicardipine Augments Local Myocardial Perfusion after Coronary Artery Reperfusion in Dogs

Nicardipine Augments Local Myocardial Perfusion after Coronary Artery Reperfusion in Dogs

Intra- and Post-Operative Assessment of Renal Cortical Perfusion by Laser Doppler Flowmetry in Renal Transplantation in the Rat

Different impact of normo- and hypotensive brain death on renal macro- and microperfusion--an experimental evaluation in a porcine model

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