Continuous Lactic Acid Production from Raw Starch in a Fermentation System Using a Reversibly Soluble-autoprecipitating Amylase and Immobilized Cells of<i>Lactobacillus casei</i>

Hoshino, Kazuhiro; Taniguchi, Masayuki; Marumoto, Hideji; Shimizu, Kazuyuki; Fujii, Michihiro

doi:10.1080/00021369.1991.10870615

Cited by 6 publications

(2 citation statements)

References 2 publications

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“…This initial thermal treatment generates high energy consumption, largely increasing the overall process cost and market price of the final product [6][7][8]. It has been estimated that energy demand of the pre-cooking step is equivalent to 30-40% of total process costs [9]. Hence, reducing the energy consumption is needed to make the starch-based processes more economically feasible.…”

Section: Introductionmentioning

confidence: 99%

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

et al. 2020

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Sustainable economy drives increasing demand for raw biomass-decomposing enzymes. Microbial expression platforms exploited as cellular factories of such biocatalysts meet requirements of large-volume production. Previously, we developed Yarrowia lipolytica recombinant strains able to grow on raw starch of different plant origin. In the present study, we used the most efficient amylolytic strain as a microbial cell factory of raw-starch-digesting (RSD) amylolytic preparation composed of two enzymes. The RSD-preparation was produced in fed-batch bioreactor cultures. Concentrated and partly purified preparation was then tested in simultaneous saccharification and fermentation (SSF) processes with thermotolerant Kluyveromyces marxianus for ethanol production and Lactobacillus plantarum for production of lactic acid. These processes were conducted as a proof-of-concept that application of the novel RSD-preparation supports sufficient starch hydrolysis enabling microbial growth and production of targeted molecules, as the selected strains were confirmed to lack amylolytic activity. Doses of the preparation and thermal conditions were individually adjusted for the two processes. Additionally, ethanol production was tested under different aeration strategies; and lactic acid production process was tested in thermally pre-treated substrate, as well. Conducted studies demonstrated that the novel RSD-preparation provides satisfactory starch hydrolyzing activity for ethanol and lactic acid production from starch by non-amylolytic microorganisms.

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Section: Introductionmentioning

confidence: 99%

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

et al. 2020

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“…[8][9][10][11] Recently, Hoshino and his coworkers reported a paper on continuous lactic acid production from raw starch, a reversibly soluble and autoprecipitating amylase and immobilized cells of Lactobacillus casei. 12) In the present paper, the fermentative pro duction of calcium lactate from white rice bran, a kind of raw rice starch, by using Streptococcus …”

mentioning

confidence: 99%

A Novel Method of Fermentative Production of Calcium Lactate Directly from Noncooked White Rice Bran.

Шамцян

Ōkubo

Murai

et al. 1993

Journal of the Japanese Society of Starch Science

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Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application

Radosavljević

Pejin

Pribić

et al. 2019

Appl Microbiol Biotechnol

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Continuous Lactic Acid Production from Raw Starch in a Fermentation System Using a Reversibly Soluble-autoprecipitating Amylase and Immobilized Cells ofLactobacillus casei

Cited by 6 publications

References 2 publications

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

A Novel Method of Fermentative Production of Calcium Lactate Directly from Noncooked White Rice Bran.

Utilization of brewing and malting by-products as carrier and raw materials in l-(+)-lactic acid production and feed application

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