Continuous in Vivo Monitoring of Amino Acid Neurotransmitters by Microdialysis Sampling with Online Derivatization and Capillary Electrophoresis Separation

Zhou, Simon Yuji.; Zuo, Hong Xiang; Stobaugh, John F.; Lunte, Craig E.; Lunte, Susan M.

doi:10.1021/ac00099a017

Cited by 197 publications

(140 citation statements)

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“…It is necessary to have at least 16-18 h of incubation to obtain stable and well detected FITC derivatives [29,30]. CE-LIFD analysis of FITC derivatives gives subattomolar determination of AA mixture, which represents few thousands of injected molecules [14,27,29,39,40].…”

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

“…This technical improvement of the microdialysis and CE coupling requires very short derivatization periods. Therefore, it is critical to use derivatizing agents such as OPA [52][53][54] or NAD [39,55,56] to produce the fluorescent derivatives in reaction times as short as a minute. This it is not possible using FITC.…”

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

“…This it is not possible using FITC. The on-line derivatization can also be performed in a conscious animal [39].…”

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

“…They can be injected directly into the analytical system, making the deproteination procedures unnecessary. To speed up the analysis and prevent losses of sample, the derivatization can be done coupling microdialysis on-line [39,52,53,55,56].…”

Section: Ce-lifd Coupled To Microdialysis Studies Neuroactive Substamentioning

confidence: 99%

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Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

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Section: Detection Techniques Coupled To Cementioning

confidence: 99%

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

“…This it is not possible using FITC. The on-line derivatization can also be performed in a conscious animal [39].…”

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

Section: Ce-lifd Coupled To Microdialysis Studies Neuroactive Substamentioning

confidence: 99%

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Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

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“…5,6 A number of methods have already been proposed for measuring L-glutamate, including chromatographic technique 7 and capillary electrophoresis. 8,9 However, these methods require time for derivatization and column separation.…”

mentioning

confidence: 99%

Microfabricated On-Line Sensor for Continuous Monitoring of l-Glutamate

Niwa

Horiuchi

Kurita³

et al. 1998

ANAL. SCI.

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Various methods have been developed for measuring the in vivo and in vitro concentrations of L-glutamate, since glutamate plays an important role in synaptic efficacy, including the long-term potentiation (LTP) 1,2 and long-term depression (LTD) 3,4 of excitatory neurotransmission. It is also reported to be a factor in several diseases including ischemia and nerve cell death.5,6 A number of methods have already been proposed for measuring L-glutamate, including chromatographic technique 7 and capillary electrophoresis. 8,9 However, these methods require time for derivatization and column separation.Recently, many groups have been focusing on the development of amperometric enzyme sensors using glutamate dehydrogenase and glutamate oxidase. The latter enzyme has more often been used to fabricate glutamate sensors because a glutamate dehydrogenase sensor requires the addition of NAD + . 10-12 Therefore, glutamate oxidase has been more often used to fabricate electrochemical sensors for detecting the neurotransmitter, L-glutamate. [13][14][15][16][17][18][19][20][21][22][23][24] Carbon fiber or metal wire microelectrodes modified with glutamate oxidase have been employed for measuring L-glutamate in the brain and in cultured rat brain tissue. [13][14][15][16] It has been reported that L-glutamate increases as the result of KCl and electrical stimulations. The advantages of the enzyme-modifed electrodes are their fast response and their ability to measure a small localized area.By contrast, on-line L-glutamate sensors have been developed. These consist of a syringe pump, a microdialysis (MD) sampling probe, an enzymatic reactor and an electrode in a flow cell. [17][18][19][20][21][22] On-line sensors are particularly useful for measuring in vivo L-glutamate, since they are very stable. This is because the flow measurement is very reproducibile and the MD membrane eliminates high molecular weight interferents such as proteins. The reported detection limits of online L-glutamate sensors are from 10 nM to 0.5 µM, which are lower than those of microelectrode-based sensors. However, the on-line sensor response is slower than that of the microelectrode sensors and it has a large sampling probe (usually 1 -3 mm long and a few hundred µm in diameter). There have been a few reports on the use of on-line sensors for measuring cultured nerve cells. However, their slow response and large sampling probe size limits their spatial and temporal resolutions in spite of their high sensitivity and long term stability. 21,22 We reported an on-line L-glutamate sensor for measuring dissociated nerve cell cultures. 23 The sensor consists of a glass capillary for sampling combined with an electrochemical thin layer flow cell. Since the sampling probe is much smaller than the MD probe, we can achieve better spatial resolution. We observed the transient release of L-glutamate by stimulating the inhibitory neurotransmitter, γ-aminobutyric acid (GABA), using this sensor. 24 However, the relatively large volume of the sensor means that there ...

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Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography

Wang

Zou

Zhang

1998

Biomed. Chromatogr.

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Continuous in Vivo Monitoring of Amino Acid Neurotransmitters by Microdialysis Sampling with Online Derivatization and Capillary Electrophoresis Separation

Cited by 197 publications

References 0 publications

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Microfabricated On-Line Sensor for Continuous Monitoring of l-Glutamate

Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography

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