Continuous, high-throughput production of artemisinin-loaded supramolecular cochleates using simple off-the-shelf flow focusing device

Shuddhodana,; Wong, Pooi Wen Kathy; Judeh, Zaher M. A.

doi:10.1016/j.msec.2019.110410

Cited by 10 publications

(5 citation statements)

References 34 publications

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“…Using Image J software, the average widths and lengths of these cylindrical structures were estimated to be 42.7±5.16 nm and 420.36±28.91 nm, respectively. Accordingly, SEM images confirm the external distinguishable shape of nanocochleates in which the length to width aspect ratio was markedly changed from spherical liposomes as previously reported [46,61].…”

Section: Morphological Examinationsupporting

confidence: 87%

“…The entrapment efficiency (EE%), drug loading (DL) and overall product yield (%) were calculated as follows [46]: The morphology of the optimized R-Lipo and R-CO formulations was investigated by scanning electron microscope (SEM, JSM-IT200; JEOL, Tokyo, Japan). A droplet of the sample suspension in de-ionized water was mounted on an aluminum stub, excess liquid was removed.…”

Section: Entrapment Efficiency Drug Loading and Product Yieldmentioning

confidence: 99%

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Enhanced oral permeability of Trans-Resveratrol using nanocochleates for boosting anticancer efficacy; in-vitro and ex-vivo appraisal

El-Melegy

Eltaher

Gaballah

et al. 2021

European Journal of Pharmaceutics and Biopharmaceutics

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Section: Morphological Examinationsupporting

confidence: 87%

Section: Entrapment Efficiency Drug Loading and Product Yieldmentioning

confidence: 99%

Enhanced oral permeability of Trans-Resveratrol using nanocochleates for boosting anticancer efficacy; in-vitro and ex-vivo appraisal

El-Melegy

Eltaher

Gaballah

et al. 2021

European Journal of Pharmaceutics and Biopharmaceutics

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“…The general method to prepare cochleates [ 44 ] is to first obtain uniform liposomes and then expose these spherical vesicles to divalent cations to form cylindrical cochleates. Lipids, dioleoylphosphatidylserine, and dimyristoylphosphatidylserine in 9:1 molar ratio were solubilized in chloroform and methanol (5:1 v/v).…”

Section: Methodsmentioning

confidence: 99%

Cochleate‐Doped Liquid Crystal as Switchable Metamaterial Window Mediated by Molecular Orientation Modified Aggregation

Madhuri

Shuddhodana

Judeh

et al. 2020

Part & Part Syst Charact

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A diversity of fascinating hollow and porous micro and nanoparticles are being developed for drug delivery purposes; however, their applications in other systems are barely touched. When filled by an active material, they form a tunable photonic metamaterial. Here, a small concentration of organic hollow cylinders called cochleates embedded into liquid crystals (LCs) is demonstrated to form a new switchable composite to control light scattering with voltage and temperature. The biocompatible cochleates, which are hollow, tube‐like, and hydrophilic in nature, can trap the liquid crystal molecules thus altering the system behavior. A switching device as thin as 6 µm with 12 wt% cochleates concentration is shown to be adequate to reveal a switchable privacy window. Possible molecular arrangement of the liquid crystal within the cochleate particles is suggested, and unexpected increase of the scattering with temperature depending on the applied voltage is observed as a result of change in the cochleate aggregation depending on the LC molecule orientation.

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“…In the first studies on cochleate formation, isolated phosphatidylserine from bovine brain with high purity has been used ( 1 , 2 ), but synthetic phospholipids, especially DOPS, have then mostly been applied to prepare cochleates ( 6 – 8 , 12 ) and especially for structural analysis ( 3 , 13 , 14 ). From an industrial perspective of drug development, costs and availability of the excipients are highly relevant and cheaper alternatives such as naturally derived phosphatidylserines are of interest especially for oral drug delivery ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

“…Different methods for the preparation of cochleate suspensions have been described in the literature ( 3 , 12 , 13 , 25 ) where the simple addition of calcium chloride solution to the PS-vesicle suspension (“trapping method”) was selected as a starting point in this study. However, as standardization of manual addition of the calcium chloride solution is difficult to achieve, preparation of cochleate suspensions by using a computer-controlled mixing device and by dialysis was evaluated ( Scheme 2 ).…”

Section: Introductionmentioning

confidence: 99%

Feasibility of the preparation of cochleate suspensions from naturally derived phosphatidylserines

Kristensen,

Hassan,

Andersen

et al. 2023

Front. Med. Technol.

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IntroductionCochleates are cylindrical particles composed of dehydrated phospholipid bilayers. They are typically prepared by addition of calcium ions to vesicles composed of negatively charged phospholipids such as phosphatidylserines (PS). Due to their high physical and chemical stability, they provide an interesting alternative over other lipid-based drug formulations for example to improve oral bioavailability or to obtain a parenteral sustained-release formulation.MethodsIn the present study, the feasibility to prepare cochleate suspensions from soy lecithin-derived phosphatidylserines (SPS) was investigated and compared to the “gold standard” dioleoyl-phosphatidylserine (DOPS) cochleates. The SPS lipids covered a large range of purities between 53 and >96% and computer-controlled mixing was evaluated for the preparation of the cochleate suspensions. Electron microscopic investigations were combined with small-angle x-ray diffraction (SAXD) and Laurdan generalized polarization (GP) analysis to characterize particle structure and lipid organization.ResultsDespite some differences in particle morphology, cochleate suspensions with similar internal lipid structure as DOPS cochleates could be prepared from SPS with high headgroup purity (≥96%). Suspensions prepared from SPS with lower purity still revealed a remarkably high degree of lipid dehydration and well-organized lamellar structure. However, the particle shape was less defined, and the typical cochleate cylinders could only be detected in suspensions prepared with higher amount of calcium ions. Finally, the study proves the feasibility to prepare suspensions of cochleates or cochleate-like particles directly from a calcium salt of soy-PS by dialysis.

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Continuous, high-throughput production of artemisinin-loaded supramolecular cochleates using simple off-the-shelf flow focusing device

Cited by 10 publications

References 34 publications

Enhanced oral permeability of Trans-Resveratrol using nanocochleates for boosting anticancer efficacy; in-vitro and ex-vivo appraisal

Enhanced oral permeability of Trans-Resveratrol using nanocochleates for boosting anticancer efficacy; in-vitro and ex-vivo appraisal

Cochleate‐Doped Liquid Crystal as Switchable Metamaterial Window Mediated by Molecular Orientation Modified Aggregation

Feasibility of the preparation of cochleate suspensions from naturally derived phosphatidylserines

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