Continuous high-frequency stimulation in freely moving rats: Development of an implantable microstimulation system

Harnack, Daniel; Meissner, Wassilios G.; Paulat, Raik; Hilgenfeld, Hannes; Müller, Wolf‐Dieter; Winter, Christine; Morgenstern, Rudolf; Kupsch, Andreas

doi:10.1016/j.jneumeth.2007.08.019

Cited by 47 publications

(41 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have found a reversal of limb-use asymmetry and an improvement in treadmill locomotion in 6-OHDA lesioned rats during STN-DBS [136, 137]. The development of instrumentation for freelymoving animals, such as an implantable microstimulation system [64] or a carry-on stimulator (described herein, see Figure 7), promises many more data on functional improvements. Using an implantable microstimulation system, Harnack et al [138] demonstrated a preservation of dopaminergic nigral neurons in a 6-OHDA rat model with progressive Parkinsonism using chronic STN-DBS.…”

Section: Biochemical and Functional Dbs Effectsmentioning

confidence: 93%

“…This impedance changes shortly after electrode implantation and over time. An electrically insulating glial sheath forms around the stimulation electrodes in patients [52, 63] and in laboratory animals [64]. This sheath is presumably responsible for the increase of electrode impedance after DBS surgery [65, 66].…”

Section: Electrochemical Considerations In the Context Of Dbsmentioning

confidence: 99%

“…This requires a small apparatus with low weight at the expense of a shorter battery life. Stimulation parameters can be adjusted from outside of the animal [64]. As a third option, the animal permanently carries the whole instrumentation in a backpack (Figure 7).…”

Section: Optimization Strategies For Experimental Dbsmentioning

confidence: 99%

See 2 more Smart Citations

Optimizing a Rodent Model of Parkinson's Disease for Exploring the Effects and Mechanisms of Deep Brain Stimulation

Nowak

Mix

Gimsa

et al. 2011

Parkinson's Disease

View full text Add to dashboard Cite

Deep brain stimulation (DBS) has become a treatment for a growing number of neurological and psychiatric disorders, especially for therapy-refractory Parkinson's disease (PD). However, not all of the symptoms of PD are sufficiently improved in all patients, and side effects may occur. Further progress depends on a deeper insight into the mechanisms of action of DBS in the context of disturbed brain circuits. For this, optimized animal models have to be developed. We review not only charge transfer mechanisms at the electrode/tissue interface and strategies to increase the stimulation's energy-efficiency but also the electrochemical, electrophysiological, biochemical and functional effects of DBS. We introduce a hemi-Parkinsonian rat model for long-term experiments with chronically instrumented rats carrying a backpack stimulator and implanted platinum/iridium electrodes. This model is suitable for (1) elucidating the electrochemical processes at the electrode/tissue interface, (2) analyzing the molecular, cellular and behavioral stimulation effects, (3) testing new target regions for DBS, (4) screening for potential neuroprotective DBS effects, and (5) improving the efficacy and safety of the method. An outlook is given on further developments of experimental DBS, including the use of transgenic animals and the testing of closed-loop systems for the direct on-demand application of electric stimulation.

show abstract

Section: Biochemical and Functional Dbs Effectsmentioning

confidence: 93%

Section: Electrochemical Considerations In the Context Of Dbsmentioning

confidence: 99%

See 1 more Smart Citation

Optimizing a Rodent Model of Parkinson's Disease for Exploring the Effects and Mechanisms of Deep Brain Stimulation

Nowak

Mix

Gimsa

et al. 2011

Parkinson's Disease

View full text Add to dashboard Cite

show abstract

“…As an alternative, relatively large stimulators were implanted at the expense of an invasive surgery [21]. Here, we use chronic DBS in a rat model under spontaneous movement conditions.…”

Section: Introductionmentioning

confidence: 99%

Impedance detection of the electrical resistivity of the wound tissue around deep brain stimulation electrodes permits registration of the encapsulation process in a rat model

Badstuebner

Stubbe

Kroeger

et al. 2017

Journal of Electrical Bioimpedance

View full text Add to dashboard Cite

An animal model of deep brain stimulation (DBS) was used in in vivo studies of the encapsulation process of custom-made platinum/iridium microelectrodes in the subthalamic nucleus of hemiparkinsonian rats via electrical impedance spectroscopy. Two electrode types with 100-µm bared tips were used: i) a unipolar electrode with a 200-µm diameter and a subcutaneous gold wire counter electrode and ii) a bipolar electrode with two parallelshifted 125-µm wires. Miniaturized current-controlled pulse generators (130 Hz, 200 µA, 60 µs) enabled chronic DBS of the freely moving animals. A phenomenological electrical model enabled recalculation of the resistivity of the wound tissue around the electrodes from daily in vivo recordings of the electrode impedance over two weeks. In contrast to the commonly used 1 kHz impedance, the resistivity is independent of frequency, electrode properties, and current density. It represents the ionic DC properties of the tissue. Significant resistivity changes were detected with a characteristic decrease at approximately the 2nd day after implantation. The maximum resistivity was reached before electrical stimulation was initiated on the 8th day, which resulted in a decrease in resistivity. Compared with the unipolar electrodes, the bipolar electrodes exhibited an increased sensitivity for the tissue resistivity.

show abstract

“…Several publications have reported the development and use of rodent stimulators for use in preclinical research (de Haas et al, 2012; Ewing et al, 2013; Forni et al, 2012; Harnack et al, 2008; Millard and Shepherd, 2007; Winter et al, 1998). Unfortunately, the description of these devices rarely contains sufficient detail to enable the faithful replication of them in independent laboratories.…”

Section: Introductionmentioning

confidence: 99%

An inexpensive, charge-balanced rodent deep brain stimulation device: A step-by-step guide to its procurement and construction

Ewing

Lipski

Grace

et al. 2013

Journal of Neuroscience Methods

Self Cite

View full text Add to dashboard Cite

Background Despite there being a relatively large number of methods papers which detail specifically the development of stimulation devices, only a small number of reports involve the application of these devices in freely moving animals. To date multiple preclinical neural stimulators have been designed and described but have failed to make an impact on the methods employed by the majority of laboratories studying DBS. Thus, the overwhelming majority of DBS studies are still performed by tethering the subject to an external stimulator. We believe that the low adoption rate of previously described methods is a result of the complexity of replicating and implementing these methods. New Method Here were describe both the design and procurement of a simple and inexpensive stimulator designed to be compatible with commonly used, commercially available electrodes (Plastics 1). Results This system is initially programmable in frequency, pulsewidth and current amplitude, and delivers biphasic, charge-balanced output to two independent electrodes. Comparison with Existing Method(s) It is easy to implement requiring neither subcutaneous implantation or custom-made electrodes and has been optimized for either direct mounting to the head or for use with rodent jackets. Conclusions This device is inexpensive and universally accessible, facilitating high throughput, low cost, long-term rodent deep brain stimulation experiments.

show abstract

Continuous high-frequency stimulation in freely moving rats: Development of an implantable microstimulation system

Cited by 47 publications

References 44 publications

Optimizing a Rodent Model of Parkinson's Disease for Exploring the Effects and Mechanisms of Deep Brain Stimulation

Optimizing a Rodent Model of Parkinson's Disease for Exploring the Effects and Mechanisms of Deep Brain Stimulation

Impedance detection of the electrical resistivity of the wound tissue around deep brain stimulation electrodes permits registration of the encapsulation process in a rat model

An inexpensive, charge-balanced rodent deep brain stimulation device: A step-by-step guide to its procurement and construction

Contact Info

Product

Resources

About