Continuous-flow zone electrophoresis: experimental demonstration and discussion of potential clinical and research applications.

Abstract

We report continuous-flow sample application and continuous electrophoretic separation on a moving cellulose acetate tape. This very simple automated electrophoretic system requires minimum electronic control circuitry. Samples and calibrators are pumped by a peristaltic pump from sample containers onto a continuously moving cellulose acetate tape. Applied samples are interspaced with rinse solution (buffer) and small air segments for sample integrity. Electrophoretic separation, staining, and destaining are all continuous functions performed on the continuously moving tape. The system appears suitable for separation of proteins. Continuous-flow application could be combined with discrete sample application onto the same tape for continuous counter-immunoelectrophoresis; moreover, counter-electrophoresis of two continuously applied proteins, with one applied in a gradient, could potentially produce a system for automated gradient counter-immunoelectrophoresis. It should also be possible to design a simple system for automated immunofixation electrophoresis.