Continuous flow production of cationic liposomes at high lipid concentration in microfluidic devices for gene delivery applications

Balbino, Tiago Albertini; Aoki, Nayla T.; Malfatti-Gasperini, Antonio A.; Oliveira, Cristiano L. P.; Azzoni, Adriano Rodrigues; Cavalcanti, Leide P.; Torre, Lucimara Gaziola de la

doi:10.1016/j.cej.2013.04.053

Cited by 93 publications

(73 citation statements)

References 36 publications

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“…Em trabalhos anteriores de nosso grupo de pesquisa, lipossomas catiônicos foram produzidos com diâmetros hidrodinâmicos médios, polidispersidade e potencial zeta adequados para a aplicação em terapia gênica (Balbino et al, 2013). Em vista disso, neste trabalho, realizou-se um estudo exploratório para produção de lipossomas catiônicos, buscando-se em seguida estender o método utilizado para produzir lipossomas funcionalizados com PEG.…”

Section: Resultsunclassified

Rotas Microfluídicas Para a Produção De Lipossomas Do Tipo Stealth

Perli¹,

Pessoa²,

Torre³

2017

Blucher Chemical Engineering Proceedings

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RESUMO -A microfluídica é uma ciência e tecnologia que tem se apresentado como uma ferramenta importante na produção de micro e nanoestruturas, a partir do escoamento de pequenas quantidades de fluídos em dispositivos com dimensões bastante reduzidas. Dentre as nanoestruturas que podem ser produzidas, reporta-se na literatura a formação de lipossomas catiônicos através de plataformas de focalização hidrodinâmica, nas quais, a componente lipídica (em etanol) é injetada em um canal central sofrendo focalização lateral pela fase aquosa. O principal objetivo deste trabalho foi produzir lipossomas catiônicos contendo polietilenoglicol (PEG) em dispositivo microfluídico. Para isso, foram investigadas duas estratégias: (1) promovendo a formação de lipossomas com PEG em apenas uma etapa e (2) a partir, da inserção posterior de lipídeo ligado ao PEG em lipossomas pré-formados. Quando produzidos através da primeira estratégia as nanoestruturas apresentaram 150,09 ± 22,14 nm de diâmetro hidrodinâmico médio e +63,00 ± 7,03 mV de potencial zeta. Como modelo padrão, realizou-se um estudo comparativo com métodos convencionais de produção (bulk), envolvendo a técnica de ultrassonicação. Nesse contexto, este trabalho visou contribuir com o desenvolvimento de processos microfluídicos de produção de lipossomas para aplicação em sistemas de liberação controlada de genes e fármacos. INTRODUÇÃOA terapia gênica é uma estratégia terapêutica que atua a partir da introdução de material genético às células-alvo, visando correção ou inativação dos genes responsáveis por uma patologia. Entretanto, devido ao tamanho, caráter aniônico e instabilidade química do material genético frente aos fluídos celulares, sua interiorização no núcleo celular (transfecção) é dificultada (Corsi et al., 2003).A fim de aumentar a taxa de transfecção in vitro e in vivo, diversos sistemas de entrega controlada de ácidos nucleicos tem sido estudados. Dos quais, destacam-se os lipossomas catiônicos, cujas estruturas são aproximadamente esféricas e formadas a partir da autoagregação de lipídeos anfifílicos em lamelas. Devido ao seu caráter catiônico, esses lipossomas podem interagir eletrostaticamente com material genético (carregado negativamente), formando lipoplexos que apresentam aptidão para adentrar as células, em consequência da interação eletrostática dos lipoplexos com a membrana celular aniônica (Azzazy, Mansour e Kazmierczak, 2006). Além do caráter catiônico, os lipossomas em questão apresentam boa compatibilidade e facilidade de modificação química de sua superfície, o que permite a produção de lipossomas

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Section: Resultsunclassified

Rotas Microfluídicas Para a Produção De Lipossomas Do Tipo Stealth

Perli¹,

Pessoa²,

Torre³

2017

Blucher Chemical Engineering Proceedings

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“…Research in the ethanol injection method was continued by different groups demonstrating the feasibility in increasing the lipid concentration in the final liposome (Trevisan et al 2011) or adapting this technique to microfluidics (Balbino et al 2013;Jahn et al 2004Jahn et al , 2007Jahn et al , 2008Jahn et al , 2010. In this case, the organic phase (soluble in water) containing the lipids is pumped into the microchannel and it is hydrodynamic compressed by two aqueous streams.…”

Section: Methods For Liposome Productionmentioning

confidence: 99%

“…CO 2 is the most common solvent used in this technique (conditions to critical (Balbino et al 2013) (b) for liposome production point are 31.1 °C and 73.8 bar) and the solvent removal is facilitated by changing the vessel pressure. Dense gases present solvent properties similar to liquids and mass transport properties similar to gases.…”

Section: Methods For Liposome Productionmentioning

confidence: 99%

Food Nanoscience and Nanotechnology

Hernández‐Sánchez¹,

Fidel²,

Gutiérrez-Ĺopez³

2015

Food Engineering Series

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Springer's Food Engineering Series is essential to the Food Engineering profession, providing exceptional texts in areas that are necessary for the understanding and development of this constantly evolving discipline. The titles are primarily reference-oriented, targeted to a wide audience including food, mechanical, chemical, and electrical engineers, as well as food scientists and technologists working in the food industry, academia, regulatory industry, or in the design of food manufacturing plants or specialized equipment. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper PrefaceFood nanoscience and nanotechnology are emergent disciplines that have grown enormously in the last years due to the attractive properties that a number of foodrelated materials have at the nanoscale. Understanding basic principles of such properties constitutes the basis for building a robust knowledge base for developing products with improved and novel characteristics.Understanding fundamentals of food nanotechnology represents a huge challenge for universities, industries and the public sector. The complex mechanisms involved in the research, development, production and legislation of food nanoproducts are studied under multi-and inter-disciplinary scopes. Bearing this in mind, this book was conceived.This book aims to contribute to basic and applied knowledge on these fields by presenting recent advances in selected topics of food nanoscience and nanotechnology, and is divided into a total of 17 chapters. The first chapter presents an overview to the field; the following chapter discusses general techniques for studying foodrelated nanomaterials, followed by six chapters on specific techniques for preparing food nanomaterials while the next contribution on dispersed systems illustrates this important and vast field. The book continues with three chapters on food nanocomposites, including those for packing purposes, and two chapters on advanced aspects of food nan...

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“…Among these, micro-hydrodynamic focusing was used to fabricate monodisperse liposomes with highly controllable size and encapsulation efficiency through buffer-organic phase flow rate ratios [134]. Another recent advancement in microfluidic-based synthesis is the NanoAssemblr platform and the NanoAssemblr scale up platform, which can produce liposomes with high precision and reproducibility both at laboratory and clinical scales [135]. …”

Section: Lipid-based Nanomaterialsmentioning

confidence: 99%

Evolution and clinical translation of drug delivery nanomaterials

et al. 2017

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With the advent of technology, the role of nanomaterials in medicine has grown exponentially in the last few decades. The main advantage of such materials has been exploited in drug delivery applications, due to their effective targeting that in turn reduces systemic toxicity compared to the conventional routes of drug administration. Even though these materials offer broad flexibility based on targeting tissue, disease, and drug payload, the demand for more effective yet highly biocompatible nanomaterial-based drugs is increasing. While therapeutically improved and safe materials have been introduced in nanomedicine platforms, issues related to their degradation rates and bio-distribution still exist, thus making their successful translation for human use very challenging. Researchers are constantly improving upon novel nanomaterials that are safer and more effective not only as therapeutic agents but as diagnostic tools as well, making the research in the field of nanomedicine ever more fascinating. In this review stress has been made on the evolution of nanomaterials that have been approved for clinical applications by the United States Food and Drug Administration Agency (FDA).

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Continuous flow production of cationic liposomes at high lipid concentration in microfluidic devices for gene delivery applications

Cited by 93 publications

References 36 publications

Rotas Microfluídicas Para a Produção De Lipossomas Do Tipo Stealth

Rotas Microfluídicas Para a Produção De Lipossomas Do Tipo Stealth

Food Nanoscience and Nanotechnology

Evolution and clinical translation of drug delivery nanomaterials

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